CCR5 Antibody

Images

 
Western Blot: CCR5 Antibody [NB100-56337] - analysis of 10 ug of total cell lysate from MCF-7 cells with at 2 ug/ml dilution
Simple Western: CCR5 Antibody [NB100-56337] - Simple Western lane view shows a specific band for CCR5 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Concentration
0.5 mg/ml

Order Details

CCR5 Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids within 300 and 350 of human CCR5 was used as immunogen.
Specificity
In MCF-7, a 42 kDa band should be observed.
Clonality
Polyclonal
Host
Rabbit
Gene
CCR5
Purity
Protein G purified
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Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Simple Western 1:10
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Reactivity Notes

Cross reacts with Human.

Alternate Names for CCR5 Antibody

  • C-C CKR-5
  • C-C motif chemokine receptor 5 A159A
  • CCCKR5
  • CC-CKR-5FLJ78003
  • CCR5
  • CCR-5
  • CD195 antigen
  • CD195
  • chemokine (C-C motif) receptor 5
  • chemokine receptor CCR5
  • chemr13
  • CKR5
  • CKR-5
  • HIV-1 fusion coreceptor
  • IDDM22CMKBR5C-C chemokine receptor type 5

Background

Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Chemokines activate leukocytes by binding to G protein coupled receptors (1). The ever-growing chemokine receptor subtypes can be divided into 2 major groups, CXCR and CCR, based on the 2 major classes of chemokines. One of the CCR receptors, CCR1, is expressed on neutrophils, monocytes, lymphocytes, and eosinophils and binds the leukocyte chemoattractant and hemopoiesis regulator macrophage-inflammatory protein (MIP-1 ), eotaxin, as well as several other related chemokines (2-4). Mice lacking the chemokine receptor CCR1 have defects in neutrophil trafficking and proliferation (5,6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on CCR5

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol CCR5
Entrez
Uniprot

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