CCR4 Antibody

Immunohistochemistry-Paraffin: CCR4 Antibody [NB100-717] - Section of human spleen.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

CCR4 Antibody Summary

• Immunogen: A synthetic peptide [SNYYLYESIPKPCTKEGIKAFGE] corresponding to amino acids 17-39 of the extracellular domain of human CCR4.
• Localization: Cell membrane
• Specificity: Peptide sequence is < 50 % identical to other human chemokine receptors in this region.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCR4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:30000
  • Flow Cytometry 1:12
  • Immunocytochemistry/ Immunofluorescence 1:400
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:500
• Application Notes: ICC/IF, IHC-F reactivity reported in (PMID: 23664188). Flow reactivity reported in (PMID: 15969628).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Monkey.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM KHPO4, 0.14M NaCl and 1.0 mg/ml BSA.
• Preservative: 0.1% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CCR4 Antibody

• C-C chemokine receptor type 4
• C-C CKR-4
• CC-CKR-4HGCN:14099
• CCR4
• CCR-4
• CD194 antigen
• CD194
• chemokine (C-C motif) receptor 4
• chemokine (C-C) receptor 4
• ChemR13
• CKR4MGC88293
• CMKBR4k5-5
• K5-5

Background

Chemokine receptors are G protein-coupled receptors (GPCRs) that play a key role in inflammation and are characterized by similar sequences with a 4 cysteine motif. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation. Receptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR4 Antibody (NB100-717)(7)

Publications using NB100-717

• Ling Z, Li W, Hu J et al. Targeting CCL2-CCR4 axis suppress cell migration of head and neck squamous cell carcinoma Cell Death & Disease 2022-02-17 [PMID: 35177591]
• Hofmann SC, Bosma A, Bruckner-Tuderman L et al. Invariant natural killer T cells are enriched at the site of cutaneous inflammation in lupus erythematosus. J Dermatol Sci 2013-04-19 [PMID: 23664188] (ICC/IF, IHC-Fr, Human)
• Yagi H, Seo N, Ohshima A, Itoh T, Itoh N, Horibe T, Yoshinari Y, Takigawa M, Hashizume H. Chemokine Receptor Expression in Cutaneous T cell and NK/T-cell Lymphomas: Immunohistochemical Staining and In Vitro Chemotactic Assay. Am J Surg Pathol;30(9):1111-9. 2006-09-01 [PMID: 16931956] (IF/IHC)
• Ritter, M et al. Elevated expression of TARC (CCL17) MDC (CCL22) in models of cigarette smoke-induced pulmonary inflammation. Biochem. Biophys. Res. Comm. 334(1): 254-262. 2005-01-01 [PMID: 15993846]
• Luttichaux, IV et al. Human adult CD34 progenitor cells functionally express the chemokine receptors CCR1, CCR4, CCR7, CXCR5, CCR10 but not CXCR4. Stem Cells Dev 14(3): 329-336. 2005-01-01 [PMID: 15969628] (FLOW)
• Shadidi, KR et al. The chemokines CCL5, CCL2, CXCL12 play significant roles in the migration of Th1 cells into rheumatiod synovial tissue. Sc. J. Immunol. 57(2): 192-198. 2003-01-01 [PMID: 12588667] (ICC/IF)
• Power, CA et al. Molecular cloning functional expression of a novel CC chemokine receptor cDNA from a human basophilic cell line. J. Biol. Chem. 270: 19495-500. 1995-01-01 [PMID: 7642634]

Bioinformatics

• Gene Symbol: CCR4
• Entrez:
  • 1233(Human)
• Uniprot:
  • P51679()