CCR4 Antibody [Unconjugated]

Western Blot shows lysates of EL‑4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/ml of Sheep Anti-Mouse CCR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5207) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR4 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 8.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CCR4 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CCR4
  Met1-Glu39, Ala99-Lys111, Ser176-Ile205, Val268-Tyr284
  Accession # P51680
• Specificity: Detects mouse CCR4 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CCR4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR4 Antibody [Unconjugated]

• C-C chemokine receptor type 4
• C-C CKR-4
• CC-CKR-4HGCN:14099
• CCR4
• CCR-4
• CD194 antigen
• CD194
• chemokine (C-C motif) receptor 4
• chemokine (C-C) receptor 4
• ChemR13
• CKR4MGC88293
• CMKBR4k5-5
• K5-5

Background

CCR4 (C-C chemokine receptor 4) is a 42 kDa member of the GPCR #1 family of transmembrane proteins. In rodent, functional CCR4 is expressed on fully differentiated CD4⁺ Th2 cells, invariant CD4⁺ NKT cells, and neurons. It is the receptor for CCL17/TARC, CCL22/MDC and CKLF1, and responds to these ligands by initiating chemotaxis. Mouse CCR4 is a 7-transmembrane protein that is 360 amino acids (aa) in length. It contains a 39 aa N-terminal extracellular domain, and a 52 aa C-terminal cyoplasmic tail that shows a poly-Ser motif between aa 343-346. Over aa sequences 1-39, 99-111, 176-205 and 268-284 collectively, mouse CCR4 shares 85% and 93% aa identity with human and rat CCR4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR4 Antibody (AF5207)(2)

Publications using AF5207

• Jagadeesh Janjanam, Giri Kumar Chandaka, Sivareddy Kotla et al. PLC3 mediates cortactin interaction with WAVE2 in MCP1-induced actin polymerization and cell migration. The American Society of Cell Biology 10/13/2015 [PMID: 26490115]
• Fang JD, Chou HC, Tung HH Endogenous expression of matriptase in neural progenitor cells promotes cell migration and neuron differentiation. J. Biol. Chem., 2010-12-13;286(7):5667-79. 2010-12-13 [PMID: 21149451] (ICC, Mouse)

Bioinformatics

• Gene Symbol: CCR4
• Uniprot:
  • P51680()