CCR9 Antibody (112509) [Unconjugated]

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CCR9 was detected in immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB179) at 25 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready, ICC/IF
Clone
112509
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

CCR9 Antibody (112509) [Unconjugated] Summary

Additional Information
Clone 112509 was used by HLDA to establish CD designation
Immunogen
BaF3 mouse pro-B cell line transfected with human CCR9
Met1-Leu369
Accession # P51686
Specificity
Specifically stains human CCR9 transfectants and MOLT-4 cells. It does not react with the parental BaF3 cells or human CCR7-transfected BaF3 cells.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CCR9
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 15-25 ug/mL
Publications
Read Publications using
MAB179 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR9 Antibody (112509) [Unconjugated]

  • C-C chemokine receptor type 9
  • C-C CKR-9
  • CC-CKR-9
  • CCR9
  • CCR-9
  • CD199
  • CDw199 antigen
  • CDw199
  • chemokine (C-C motif) receptor 9
  • G protein-coupled receptor 28
  • GPR28
  • GPR-9-6G-protein coupled receptor 28

Background

CCR9 is a G protein-linked seven transmembrane domain cytokine receptor. CCR9 serves as a receptor for CCL25/TECK. It is expressed on mature and immature thymocytes and some peripheral T and B cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCR9 Antibody (MAB179)(8)

We have publications tested in 2 confirmed species: Human, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 3 applications: Flow Cytometry, ICC, Neutralization.


Filter By Application
Flow Cytometry
(6)
ICC
(1)
Neutralization
(1)
All Applications
Filter By Species
Human
(6)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using MAB179 Applications Species
Y Hu, A Ma, S Lin, Y Yang, G Hong Novel peptide screened from a phage display library antagonizes the activity of CC chemokine receptor 9 Oncol Lett, 2017;14(6):6471-6476. 2017 [PMID: 29163684] (ICC, Human) ICC Human
Intracellular allosteric antagonism of the CCR9 receptor Nature, 2016;540(7633):462-465. 2016 [PMID: 27926729] (Flow Cytometry, Human) Flow Cytometry Human
Johnson-Holiday C, Singh R, Johnson E CCL25 mediates migration, invasion and matrix metalloproteinase expression by breast cancer cells in a CCR9-dependent fashion. Int. J. Oncol., 2011;38(5):1279-85. 2011 [PMID: 21344163] (Neutralization, Human) Neutralization Human
Minang JT, Trivett MT, Bolton DL, Trubey CM, Estes JD, Li Y, Smedley J, Pung R, Rosati M, Jalah R, Pavlakis GN, Felber BK, Piatak M, Roederer M, Lifson JD, Ott DE, Ohlen C Distribution, persistence, and efficacy of adoptively transferred central and effector memory-derived autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones in rhesus macaques during acute infection. J. Immunol., 2010;184(1):315-26. 2010 [PMID: 19949091] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque)) Flow Cytometry Primate - Macaca mulatta (Rhesus Macaque)
Bolton DL, Minang JT, Trivett MT, Song K, Tuscher JJ, Li Y, Piatak M, O'Connor D, Lifson JD, Roederer M, Ohlen C Trafficking, persistence, and activation state of adoptively transferred allogeneic and autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones during acute and chronic infection of rhesus macaques. J. Immunol., 2010;184(1):303-14. 2010 [PMID: 19949089] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque)) Flow Cytometry Primate - Macaca mulatta (Rhesus Macaque)
Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients. J. Immunol., 2007;179(6):4272-82. 2007 [PMID: 17785868] (Flow Cytometry, Human) Flow Cytometry Human
Ni Z, Campbell JJ, Niehans G, Walcheck B The monoclonal antibody CHO-131 identifies a subset of cutaneous lymphocyte-associated antigen T cells enriched in P-selectin-binding cells. J. Immunol., 2006;177(7):4742-8. 2006 [PMID: 16982914] (Flow Cytometry, Human) Flow Cytometry Human
Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL. Blood, 2003;103(7):2806-8. 2003 [PMID: 14656893] (Flow Cytometry, Human) Flow Cytometry Human

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Bioinformatics

Gene Symbol CCR9
Uniprot