CCR10/GPR2 Antibody (248918) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with (A) mouse CCR10 or (B) irrelevant transfectant and eGFP was stained with Rat Anti-Mouse CCR10 Monoclonal Antibody (Catalog # MAB2815) or Rat IgG2AIsotype Control (Catalog # MAB006, data not shown) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113). View our protocol for Staining Membrane-associated Proteins.

Raw264.7 cells were stained with Rat Anti-Mouse CCR10 Monoclonal Antibody (Catalog # MAB2815, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 248918
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CCR10/GPR2 Antibody (248918) [Unconjugated] Summary

• Immunogen: C6 rat glioma cell line transfected with mouse CCR10
  Met1-Asn362
  Accession # NP_031747
• Specificity: Detects mouse CCR10. Stains mouse CCR10-transfected cells but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCR10
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR10/GPR2 Antibody (248918) [Unconjugated]

• CC chemokine receptor 10
• C-C chemokine receptor type 10
• C-C CKR-10
• CC-CKR-10
• CCR10
• CCR-10
• chemokine (C-C motif) receptor 10
• G protein-coupled receptor 2
• GPR2
• GPR2G-protein coupled receptor 2

Background

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that functions as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR10/GPR2 Antibody (MAB2815)(7)

Publications using MAB2815

• EJ Park, PK Myint, MG Appiah, P Worawattan, J Inprasit, O Prajuabjin, ZY Soe, A Gaowa, E Kawamoto, M Shimaoka Ligand-competent fractalkine receptor is expressed on exosomes Biochemistry and Biophysics Reports, 2021-02-02;26(0):100932. 2021-02-02 [PMID: 33553692] (Flow Cytometry, Mouse)
• P Wang, X Qi, G Xu, J Liu, J Guo, X Li, X Ma, H Sun CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells Aging (Albany NY), 2019-09-26;11(18):7402-7415. 2019-09-26 [PMID: 31557129] (Neutralization, Mouse)
• NM Bath, X Ding, NA Wilson, BM Verhoven, BA Boldt, A Sukhwal, SR Reese, SE Panzer, A Djamali, RR Redfield Desensitization and treatment with APRIL/BLyS blockade in rodent kidney transplant model PLoS ONE, 2019-02-08;14(2):e0211865. 2019-02-08 [PMID: 30735519] (Flow Cytometry, Rat)
• SA Geherin, D Gómez, RA Glabman, G Ruthel, A Hamann, GF Debes IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require ?4?1 Integrin To Migrate between the Peritoneum and Inflamed Skin J. Immunol, 2016-02-05;196(6):2514-25. 2016-02-05 [PMID: 26851219] (Flow Cytometry, Mouse)
• Mitagami Y, Yasunaga J, Kinosada H, Ohshima K, Matsuoka M Interferon-gamma Promotes Inflammation and Development of T-Cell Lymphoma in HTLV-1 bZIP Factor Transgenic Mice. PLoS Pathog, 2015-08-21;11(8):e1005120. 2015-08-21 [PMID: 26296091] (Flow Cytometry, Mouse)
• Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Nature, 2011-07-13;475(7355):226-30. 2011-07-13 [PMID: 21753853] (Neutralization, Mouse)
• Marks BR, Nowyhed HN, Choi JY, Poholek AC, Odegard JM, Flavell RA, Craft J Thymic self-reactivity selects natural interleukin 17-producing T cells that can regulate peripheral inflammation. Nat. Immunol., 2009-09-06;10(10):1125-32. 2009-09-06 [PMID: 19734905] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CCR10
• Uniprot:
  • NP_031747()