CCR10/GPR2 Antibody (248918) [Allophycocyanin]

B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse/Rat CCR10 APC-conjugated Monoclonal Antibody (Catalog # FAB2815A, filled histogram) or isotype control antibody (IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Rat splenocytes were stained with Rat Anti-Mouse/Rat CCR10 APC-conjugated Monoclonal Antibody (Catalog # FAB2815A) and Anti-Rat CD3 PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (IC006A). View our protocol for Staining Membrane-associated Proteins.

Raw264.7 cells were stained with Rat Anti-Mouse/Rat CCR10 APC‑conjugated Monoclonal Antibody (Catalog # FAB2815A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

HEK293 Human Cell Line transfected with Mouse CCR10 and eGFP (A) vs irrelevant GPCR (B) were stained with Rat Anti-Mouse/Rat CCR10 APC‑conjugated Monoclonal Antibody (Catalog # fab2815a) and eGFP isotype control antibody (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu, Rt
• Applications: Flow
• Clone: 248918
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

CCR10/GPR2 Antibody (248918) [Allophycocyanin] Summary

• Immunogen: C6 rat glioma cell line transfected with mouse CCR10
  Met1-Asn362
  Accession # NP_031747
• Specificity: Detects mouse and rat CCR10. Stains mouse CCR10-transfected cells but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CCR10
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR10/GPR2 Antibody (248918) [Allophycocyanin]

• CC chemokine receptor 10
• C-C chemokine receptor type 10
• C-C CKR-10
• CC-CKR-10
• CCR10
• CCR-10
• chemokine (C-C motif) receptor 10
• G protein-coupled receptor 2
• GPR2
• GPR2G-protein coupled receptor 2

Background

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that functions as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR10/GPR2 Antibody (FAB2815A)(8)

Publications using FAB2815A

• Ying Fu, Zhiming Wang, Baichao Yu, Yuli Lin, Enyu Huang, Ronghua Liu et al. Intestinal CD11b+ B Cells Ameliorate Colitis by Secreting Immunoglobulin A Frontiers in Immunology 2021-11-03 [PMID: 34804004] (Flow Cytometry, Mouse, Transgenic Mouse)
• K Usami, K Niimi, A Matsuo, Y Suyama, Y Sakai, S Sato, K Fujihashi, H Kiyono, S Uchino, M Furukawa, J Islam, K Ito, T Moriya, Y Kusumoto, M Tomura, RC Hovey, J Sugawara, H Yoneyama, H Kitazawa, K Watanabe, H Aso, T Nochi The gut microbiota induces Peyer's-patch-dependent secretion of maternal IgA into milk Cell Reports, 2021-09-07;36(10):109655. 2021-09-07 [PMID: 34496253] (Flow Cytometry, Transgenic Mouse)
• P Wang, X Qi, G Xu, J Liu, J Guo, X Li, X Ma, H Sun CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells Aging (Albany NY), 2019-09-26;11(18):7402-7415. 2019-09-26 [PMID: 31557129] (Flow Cytometry, Mouse)
• Takahiro Nagatake, So-ichiro Hirata, Tomoaki Koga, Etsushi Kuroda, Shingo Kobari, Hidehiko Suzuki et al. BLT1 mediates commensal bacteria-dependent innate immune signals to enhance antigen-specific intestinal IgA responses Mucosal Immunology 9/1/2019 [PMID: 31142830]
• Mats Bemark, Helena Hazanov, Anneli Strömberg, Rathan Komban, Joel Holmqvist, Sofia Köster et al. Limited clonal relatedness between gut IgA plasma cells and memory B cells after oral immunization Nature Communications 9/6/2016 [PMID: 27596266]
• Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential PLoS ONE, 2016-04-27;11(4):e0154253. 2016-04-27 [PMID: 27119555] (Flow Cytometry, Mouse)
• Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Nature, 2011-07-13;475(7355):226-30. 2011-07-13 [PMID: 21753853] (Flow Cytometry, Mouse)
• Uematsu S, Fujimoto K, Jang MH, Yang BG, Jung YJ, Nishiyama M, Sato S, Tsujimura T, Yamamoto M, Yokota Y, Kiyono H, Miyasaka M, Ishii KJ, Akira S Regulation of humoral and cellular gut immunity by lamina propria dendritic cells expressing Toll-like receptor 5. Nat. Immunol., 2008-05-30;9(7):769-76. 2008-05-30 [PMID: 18516037] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CCR10
• Uniprot:
  • NP_031747()