CCL4/MIP-1 beta Antibody [Unconjugated]

Images

 
CCL4/MIP‑1 beta was detected in perfusion fixed frozen sections of mouse small intestine using Goat Anti-Mouse CCL4/MIP‑1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-451-NA) at 1.7 µg/mL ...read more
CCL4/MIP‑1 beta was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line untreated (right panel) and treated with LPS (left panel) using Goat Anti-Mouse CCL4/MIP‑1 beta Antigen ...read more
Recombinant Mouse CCL4/MIP‑1 beta (Catalog # 451‑MB) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 μg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL4/MIP‑1 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

CCL4/MIP-1 beta Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL4/MIP-1 beta
Ala24-Asn92
Accession # Q5QNV9
Specificity
Detects CCL4/MIP-1 beta in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) MIP‑1 beta  and recombinant mouse (rm) MIP-1 alpha is observed. This antibody will not neutralize the biological activity of rmMIP-1 alpha. In the chemotaxis assay this antibody will partially neutralize rhMIP-1 alpha and rhMIP-1 beta at a 10‑30 fold higher IgG concentration.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL4
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Immunocytochemistry 1-15 ug/mL
  • Neutralization 0.2-1.0 ug/mL
Publications
Read Publications using
AF-451-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL4/MIP-1 beta Antibody [Unconjugated]

  • ACT2
  • ACT-2
  • AT744.1
  • C-C motif chemokine 4
  • CCL4
  • chemokine (C-C motif) ligand 4
  • Exodus-3
  • G-26 T-lymphocyte-secreted protein
  • G-26
  • HC21
  • LAG1
  • LAG-1
  • Lymphocyte activation gene 1 protein
  • Macrophage inflammatory protein 1-beta
  • MIP1 beta
  • MIP-1 beta
  • MIP1B
  • MIP1B1
  • MIP-1-beta
  • MIP-1-beta(1-69)
  • PAT 744
  • Protein H400
  • SCYA2
  • SCYA4
  • secreted protein G-26
  • SIS-gamma
  • small inducible cytokine A4 (homologous to mouse Mip-1b)
  • Small-inducible cytokine A4
  • T-cell activation protein 2

Background

CCL4, also known as macrophage inflammatory protein 1 beta (MIP-1 beta ), is a 12 kDa beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells (1, 2). CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium (3-8). A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment (6). CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha (9). The first two N-terminal amino acids can be cleaved from human CCL4 by CD26/DPPIV (10, 11). Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2 (10). In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor (2). Both forms of CCL4 block HIV-1 infection of T cells by inducing the downregulation of CCR5 (10). Mature mouse CCL4 shares 77% and 86% aa sequence identity with human and rat CCL4, respectively.
  1. Rot, A. and U.H. von Andrian (2004) Annu. Rev. Immunol. 22:891.
  2. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
  3. Sun, X. et al. (2006) Infec. Immun. 74:5943.
  4. Bisset, L.R. and Schmid-Grendelmeier, P. (2005) Curr. Opin. Pulm. Med. 11:35.
  5. Frangogiannis, N.G. (2004) Inflamm. Res. 53:585.
  6. Bystry, R.S. et al. (2001) Nat. Immunol. 2:1126.
  7. Oliveira, S.H.P. et al. (2002) J. Leukoc. Biol. 71:1019.
  8. Schall, T.J. et al. (1993) J. Exp. Med. 177:1821.
  9. Guan, E. et al. (2001) J. Biol. Chem. 276:12404.
  10. Guan, E. et al. (2002) J. Biol. Chem. 277:32348.
  11. Guan, E. et al. (2004) J. Cell. Biochem. 92:53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL4/MIP-1 beta Antibody (AF-451-NA)(8)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 6 applications: ELISA Development, Flow Cytometry, ICC, IHC, IHC-P, Neutralization.


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ELISA Development
(1)
Flow Cytometry
(1)
ICC
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IHC
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Mouse
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Showing Publications 1 - 8 of 8.
Publications using AF-451-NA Applications Species
JM Williford, J Ishihara, A Ishihara, A Mansurov, P Hosseinchi, TM Marchell, L Potin, MA Swartz, JA Hubbell Recruitment of CD103+ dendritic cells via tumor-targeted chemokine delivery enhances efficacy of checkpoint inhibitor immunotherapy Sci Adv, 2019;5(12):eaay1357. 2019 [PMID: 31844672] (IHC, Mouse) IHC Mouse
VE Thaney, AM O'Neill, MM Hoefer, R Maung, AB Sanchez, M Kaul IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury Sci Rep, 2017;7(0):46514. 2017 [PMID: 28425451] (Neutralization, Mouse) Neutralization Mouse
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010;120(3):907-23. 2010 [PMID: 20197626] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Wu Y, Li YY, Matsushima K, Baba T, Mukaida N CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process. J. Immunol., 2008;181(9):6384-93. 2008 [PMID: 18941229] (IHC-P, Mouse) IHC-P Mouse
Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection. J. Exp. Med., 2008;205(9):2075-84. 2008 [PMID: 18695004] (Neutralization, Mouse) Neutralization Mouse
Meagher C, Arreaza G, Peters A, Strathdee CA, Gilbert PA, Mi QS, Santamaria P, Dekaban GA, Delovitch TL CCL4 protects from type 1 diabetes by altering islet beta-cell-targeted inflammatory responses. Diabetes, 2007;56(3):809-17. 2007 [PMID: 17327452] (Neutralization, Mouse) Neutralization Mouse
Cardona AE, Gonzalez PA, Teale JM CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response. Infect. Immun., 2003;71(5):2634-42. 2003 [PMID: 12704138] (ICC, Mouse) ICC Mouse
Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats. J. Immunol., 2000;164(5):2650-9. 2000 [PMID: 10679105] (ELISA Development, Rat) ELISA Development Rat

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Biotin BAF451

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Bioinformatics

Gene Symbol CCL4
Uniprot