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CCL4/MIP-1 beta Antibody [CoraFluor™ 1]

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7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser." > CoraFluor™  1, amine reactive (Catalog:<a class=7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser." title="CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser." />
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # ...read more

Product Details

Summary
Applications WB, IHC, B/N, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
CoraFluor 1

Order Details

CCL4/MIP-1 beta Antibody [CoraFluor™ 1] Summary

Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Immunogen
E. coli-derived recombinant mouse CCL4/MIP-1 beta
Ala24-Asn92
Accession # Q5QNV9
Specificity
Detects CCL4/MIP-1 beta in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) MIP‑1 beta  and recombinant mouse (rm) MIP-1 alpha is observed. This antibody will not neutralize the biological activity of rmMIP-1 alpha . In the chemotaxis assay this antibody will partially neutralize rhMIP-1 alpha and rhMIP-1 beta at a 10‑30 fold higher IgG concentration.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL4
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry
  • Immunohistochemistry
  • Neutralization
  • Western Blot
Application Notes
Recommended applications are based on validated applications from the unconjugated base product AF-451-NA. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Antigen Affinity-purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for CCL4/MIP-1 beta Antibody [CoraFluor™ 1]

  • ACT2
  • ACT-2
  • AT744.1
  • C-C motif chemokine 4
  • CCL4
  • chemokine (C-C motif) ligand 4
  • Exodus-3
  • G-26 T-lymphocyte-secreted protein
  • G-26
  • HC21
  • LAG1
  • LAG-1
  • Lymphocyte activation gene 1 protein
  • Macrophage inflammatory protein 1-beta
  • MIP1 beta
  • MIP-1 beta
  • MIP1B
  • MIP1B1
  • MIP-1-beta
  • MIP-1-beta(1-69)
  • PAT 744
  • Protein H400
  • SCYA2
  • SCYA4
  • secreted protein G-26
  • SIS-gamma
  • small inducible cytokine A4 (homologous to mouse Mip-1b)
  • Small-inducible cytokine A4
  • T-cell activation protein 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CCL4