CBX3 Antibody

Western Blot: CBX3 Antibody [NBP2-15736] - Wild-type (WT) and HP1 gamma knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HP1 gamma antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: CBX3 Antibody [NBP2-15736] - Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE gel, diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: CBX3 Antibody [NBP2-15736] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBX3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: CBX3 Antibody [NBP2-15736] - Mouse duodenum. HP1 gamma antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: CBX3 Antibody [NBP2-15736] - Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using antibody at 1:250 dilution.

Immunohistochemistry-Paraffin: CBX3 Antibody [NBP2-15736] - Mouse brain. HP1 gamma antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunoprecipitation: CBX3 Antibody [NBP2-15736] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extractB. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of HP1 gamma protein by 2 ug of HP1 gamma antibody 15% SDS-PAGE The immunoprecipitated HP1 gamma protein was detected by HP1 gamma antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CBX3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human CBX3. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CBX3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockout Validated
  • Western Blot 1:5000-1:20000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CBX3 Antibody

• chromobox homolog 3 (Drosophila HP1 gamma)
• chromobox homolog 3
• chromobox protein homolog 3
• Drosophila)
• HECH
• Heterochromatin protein 1 homolog gamma
• heterochromatin protein HP1 gamma
• heterochromatin-like protein 1
• HP1 gamma homolog
• HP1 gamma
• HP1-GAMMA
• Modifier 2 protein

Background

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CBX3