CBX3 Antibody

Images

 
Western Blot: CBX3 Antibody [NBP2-15736] - Wild-type (WT) and HP1 gamma knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HP1 gamma antibody. HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: CBX3 Antibody [NBP2-15736] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBX3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are ...read more
Immunohistochemistry-Paraffin: CBX3 Antibody [NBP2-15736] - Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using antibody at 1:250 dilution.
Western Blot: CBX3 Antibody [NBP2-15736] - Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE gel, diluted at 1:10000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
     

Genetic Strategies

   

Order Details

CBX3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HP1 gamma. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CBX3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:100
  • Immunoprecipitation 1:100-1:500
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CBX3 Antibody

  • chromobox homolog 3 (Drosophila HP1 gamma)
  • chromobox homolog 3
  • chromobox protein homolog 3
  • Drosophila)
  • HECH
  • Heterochromatin protein 1 homolog gamma
  • heterochromatin protein HP1 gamma
  • heterochromatin-like protein 1
  • HP1 gamma homolog
  • HP1 gamma
  • HP1-GAMMA
  • Modifier 2 protein

Background

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CBX3