Cathepsin D Antibody - BSA Free

Western Blot: Cathepsin D Antibody [NBP1-85562] - Analysis in human cell line SK-BR-3.

Immunohistochemistry-Paraffin: Cathepsin D Antibody [NBP1-85562] - Staining of human small intestine shows moderate to strong granular cytoplasmic positivity in glandular and lymphoid cells.

Immunohistochemistry-Paraffin: Cathepsin D Antibody [NBP1-85562] - Staining of human skeletal muscle shows occasional granular cytoplasmic positivity in a subset of myocytes.

Immunohistochemistry-Paraffin: Cathepsin D Antibody [NBP1-85562] - Staining of human fallopian tube shows moderate granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Cathepsin D Antibody [NBP1-85562] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cathepsin D Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCQSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVDQNIFSFYLSRDPDAQPGGELMLGGT
• Marker: Lysosomes Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CTSD
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Cathepsin D Protein (NBP1-85562PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Cathepsin D Antibody - BSA Free

• cathepsin D (lysosomal aspartyl protease)
• Cathepsin D
• CPSD
• CTSD
• EC 3.4.23
• EC 3.4.23.5
• lysosomal aspartyl peptidase
• lysosomal aspartyl protease
• MGC2311
• neuronal 10

Background

Cathepsin D has been reported to be involved in malignant tumour progression and with prognosis of various human cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin D Antibody (NBP1-85562)(2)

Publications using NBP1-85562

• Mastroiacovo F, Biagioni F, Lenzi P et al. Within the Ischemic Penumbra, Sub-Cellular Compartmentalization of Heat Shock Protein 70 Overlaps with Autophagy Proteins and Fails to Merge with Lysosomes Molecules (Basel, Switzerland) 2022-05-13 [PMID: 35630599]
• Ahmad Y, Boisvert FM, Lundberg E et al. Systematic analysis of protein pools, isoforms, and modifications affecting turnover and subcellular localization. Mol Cell Proteomics 2012-03-01 [PMID: 22002106]

Bioinformatics

• Gene Symbol: CTSD