Cathepsin H Antibody [Unconjugated]

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Cathepsin H was detected in perfusion fixed frozen sections of mouse ovary using 1.7 µg/mL Goat Anti-Mouse Cathepsin H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1013) overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Cathepsin H Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin H
Glu22-Val333
Accession # Q3UCD6
Specificity
Detects mouse Cathepsin H in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant mouse (rm) Cathepsin L, and rmCathepsin J is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTSH
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
Publications
Read Publications using
AF1013 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin H Antibody [Unconjugated]

  • ACC-4
  • ACC-5
  • aleurain
  • cathepsin B3
  • cathepsin BA
  • Cathepsin H
  • CPSB
  • CTSH
  • DKFZp686B24257
  • EC 3.4.22
  • EC 3.4.22.16
  • MGC1519
  • minichain
  • N-benzoylarginine-beta-naphthylamide hydrolase

Background

Cathepsin H is a lysosomal cysteine protease of the papain family (1). It is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑20), a propeptide (residues 21‑95), a mini chain (residues 96‑103), a heavy chain (residues 114‑290) and a light chain (residues 291‑333) (2, 3). A truncated form with a 12 amino acid deletion in the signal peptide region is secreted (4). Cathepsin H is the only known mono-aminopeptidase in the papain family (5). Cathepsin H expression is significantly increased in disease states such as in prostate tumors, sera of asthmatic patients, and mucosa of colorectal cancer patients (4, 6, 7).

  1. Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1089, Academic Press, San Diego. 
  2. Lafuse, W.P. et al. (1995) J. Leukoc. Biol. 57:663.
  3. Soderstrom, M. et al. (1999) Biochim. Biophys. Acta 1446:35.
  4. Waghray, A. et al. (2002) J. Biol. Chem. 277:11533.
  5. Guncar, G. et al. (1998) Structure 6:51.
  6. Cimerman, N. et al. (2001) Clin. Chim. Acta 310:113.
  7. del Re, E.C. et al. (2000) Br. J. Cancer. 82:1317.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin H Antibody (AF1013)(4)

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Bioinformatics

Gene Symbol CTSH
Entrez
Uniprot