CASZ1 Antibody - BSA Free

Western Blot: MAGP-2/MFAP5 Antibody [NBP1-77942] - Shows detection of a band ~125 kDa in size corresponding to hCas5 (arrowhead). Lane 1 contains a lysate from AS cells transfected with empty vector. Lane 2 contains lysate from AS cells transfected with hCas5 (predicted MW 125 kDa). Lane 3 contains lysate from BE2 cells that have been shown to contain a high level of hCas mRNA (as tested by Northern blot). Lane 4 contains lysate from human muscle tissue that also shows high levels of hCas mRNA. Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown). Personal Communication. Carol Thiele, NCI, Bethesda, MD.

Western blot using affinity purified anti-hCas5 antibody. Lane 1: AS cell lysate transfected with empty vector. Lane 2: AS cell lysate transfected with hCas5 (predicted MW 125kDa). Lane 3: BE2 cell lysate that have been shown to contain a high level of hCas mRNA (as tested by Northern blot). Lane 4: human muscle tissue lysate that shows high levels of hCas mRNA. Shows detection of a band ~125kDa in size corresponding to hCas5 in lane 2 (arrowhead). Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown). Personal Communication. Carol Thiele, NCI, Bethesda, MD.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CASZ1 Antibody - BSA Free Summary

• Description: This affinity-purified antibody is directed against human CASZ1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified CASZ1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to region near the amino terminal end of human CASZ1 protein. (Uniprot: B3KRV8)
• Specificity: Expect cross reactivity with multiple isoforms of this protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASZ1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:50000
  • Western Blot 1:500-1:2000
• Application Notes: This affinity-purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot, a band approximately 125 kDa in size corresponding to hCas5 protein is expected in the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross reactivity with CASZ1 from human, mouse, chimpanzee, bovine, and rat based on 100% homology with the immunizing sequence. Expect cross reactivity with multiple isoforms of this protein. Cross reactivity with CASZ1 from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CASZ1 Antibody - BSA Free

• castor homolog 1, zinc finger (Drosophila)
• castor homolog 1, zinc finger
• castor zinc finger 1
• castor
• Castor-related protein
• CST
• dJ734G22.1
• FLJ20321
• Putative survival-related protein
• SRG
• SRGcst
• survival related
• survival-related
• Zinc finger protein 693
• zinc finger protein castor homolog 1
• ZNF693
• ZNF693FLJ12223

Background

hCas5 (Castor) is the human homolog of a Drosophila gene that regulates neurogenesis. In human neuro-blastoma tumors where a loss of heterozygosity of CASZ1 is noted, hCas functions to suppress the tumorigenicity of cells. IhCas5 is a
developmentally regulated transcription factor found in neural and muscle cells. hCas5 is a Zn finger transcription factor that is predominantly found in the nucleus. This antibody is designed to facilitate the functional study of this protein and its role in developmental regulation and expression in human tumor tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CASZ1
• Uniprot:
  • B3KRV8(Human)