MRGX2 Antibody

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Product Details

Summary
Reactivity Hu, Mu, Ha(-)Species Glossary
Applications WB, ICC/IF, IHC, KD, Single-Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

MRGX2 Antibody Summary

Immunogen
A synthetic peptide from the 4th cytoplasmic loop of human Mas-related G-protein coupled receptor member X2 (MRGPRX2) conjugated to an immunogenic carrier protein was used as the antigen.
Specificity
MRGPRX2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MRGPRX2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 : 200 - 1 : 2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1 : 200 - 1 : 2000
  • Knockdown Validated
  • Single Cell Western
Application Notes
Use in ICC/IF reported in secitific publication PMID: 32391014
Control
MRGX2 Overexpression Lysate
Publications
Read Publications using
NB110-75035 in the following applications:

  • 1 publication
  • 2 publications
  • 1 publication
  • KD
    1 publication
  • WB
    4 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (Alkanfari IS et al).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.

Alternate Names for MRGX2 Antibody

  • MAS-related GPR, member X2
  • mas-related G-protein coupled receptor member X2
  • MRGPRX2
  • MRGX2
  • MRGX2G protein-coupled receptor MRGX2

Background

FUNCTION: Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14 seems to be a high potency ligand at this receptor. Cortistatin has several biological functions including roles in sleep regulation locomotor activity, and cortical function. In receptor-expressing cells, cortistatin-stimulated increases in intracellular Ca(2+) but had no effect on basal or forskolin-stimulated cAMP levels, suggesting that this receptor is G(q)-coupled. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Has a limited expression profile, both peripheral and within the central nervous system, with highest levels in dorsal root ganglion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MRGX2 Antibody (NB110-75035)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow, ICC/IF, IHC-P, KD, WB.


Filter By Application
Flow
(1)
ICC/IF
(2)
IHC-P
(1)
KD
(1)
WB
(4)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MRGX2 Antibody (NB110-75035) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional MRGX2 Products

Array NB110-75035

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Diseases for MRGX2 Antibody (NB110-75035)

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Bioinformatics

Gene Symbol MRGPRX2
Entrez
Uniprot