MRGX2 Antibody

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Product Details

Summary
Reactivity Hu, Mu, Ha(-)Species Glossary
Applications WB, ICC/IF, IHC, KD, Single-Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

MRGX2 Antibody Summary

Immunogen
A synthetic peptide from the 4th cytoplasmic loop of human Mas-related G-protein coupled receptor member X2 (MRGPRX2) conjugated to an immunogenic carrier protein was used as the antigen.
Specificity
MRGPRX2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MRGPRX2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1 : 200 - 1 : 2000
  • Knockdown Validated
  • Single Cell Western
  • Western Blot 1 : 200 - 1 : 2000
Application Notes
Use in ICC/IF reported in scientfic publication (PMID: 32391014). Flow, WB, KD reactivity reported in scientific thesis.
Publications
Read Publications using
NB110-75035 in the following applications:

  • 1 publication
  • 2 publications
  • 1 publication
  • KD
    1 publication
  • WB
    6 publications

Reactivity Notes

Mouse reactivity reported in (PMID: 34008339).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.

Notes

The 0.1mg split size of this product is provided in liquid format (reconstituted in sterile water).

Alternate Names for MRGX2 Antibody

  • MAS-related GPR, member X2
  • mas-related G-protein coupled receptor member X2
  • MRGPRX2
  • MRGX2
  • MRGX2G protein-coupled receptor MRGX2

Background

FUNCTION: Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14 seems to be a high potency ligand at this receptor. Cortistatin has several biological functions including roles in sleep regulation locomotor activity, and cortical function. In receptor-expressing cells, cortistatin-stimulated increases in intracellular Ca(2+) but had no effect on basal or forskolin-stimulated cAMP levels, suggesting that this receptor is G(q)-coupled. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Has a limited expression profile, both peripheral and within the central nervous system, with highest levels in dorsal root ganglion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-75035
Species: Hu, Mu, Ha(-)
Applications: WB, ICC/IF, IHC, KD, Single-Cell Western

Publications for MRGX2 Antibody (NB110-75035)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow, ICC/IF, IHC-P, KD, WB.


Filter By Application
Flow
(1)
ICC/IF
(2)
IHC-P
(1)
KD
(1)
WB
(6)
All Applications
Filter By Species
Human
(6)
Mouse
(3)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB110-75035 Applications Species
Sun X, Lv W, Wang Y et al. Mrgprb2 gene plays a role in the anaphylactoid reactions induced by Houttuynia cordata injection Journal of ethnopharmacology Jan 29 2022 [PMID: 35104575] (WB, Mouse) WB Mouse
Widner DB, Liu C, Zhao Q et al. Activated mast cells in skeletal muscle can be a potential mediator for cancer-associated cachexia Journal of cachexia, sarcopenia and muscle May 18 2021 [PMID: 34008339] (WB, Mouse) WB Mouse
Idahosa, CN The Mast Cell Receptor Mas-Related Gene X2 (MrgX2) in Health and Disease. Thesis. 2015 Nov 01 (IHC-P, ICC/IF, Human) IHC-P, ICC/IF Human
Alkanfari, I;Freeman, KB;Roy, S;Jahan, T;Scott, RW;Ali, H; Small-Molecule Host-Defense Peptide Mimetic Antibacterial and Antifungal Agents Activate Human and Mouse Mast Cells via Mas-Related GPCRs Cells Apr 3 2019 [PMID: 30987258] (WB, Human) WB Human
Roy S, Ganguly A, Haque M, Ali H. Angiogenic Host Defense Peptide AG-30/5C and Bradykinin B2 Receptor Antagonist Icatibant Are G Protein Biased Agonists for MRGPRX2 in Mast Cells. J. Immunol. Jan 16 2019 [PMID: 30651343] (WB, Human) WB Human
Subramanian H, Gupta K, Lee D et al. beta-Defensins Activate Human Mast Cells via Mas-Related Gene X2. J Immunol 2013 Jul 1 [PMID: 23698749] (WB, Human) WB Human
Alkanfari IS The Role of MRGPRX2/Mrgprb2 in Regulating Mast Cell Function Thesis (Flow, WB, KD, Mouse, Human) Flow, WB, KD Mouse, Human
Callahan BN, Kammala AK, Syed M et al. Osthole, a Natural Plant Derivative Inhibits MRGPRX2 Induced Mast Cell Responses Front Immunol Apr 24 2020 [PMID: 32391014] (ICC/IF, Human)

Details:
Confocal microscopy
ICC/IF Human

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Additional MRGX2 Products

Array NB110-75035

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Bioinformatics

Gene Symbol MRGPRX2
Entrez
Uniprot