MRGX2 Antibody

• Reactivity: Hu, Mu, Ha(-)
• Applications: WB, ICC/IF, IHC, KD, Single-Cell Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

MRGX2 Antibody Summary

• Immunogen: A synthetic peptide from the 4th cytoplasmic loop of human Mas-related G-protein coupled receptor member X2 (MRGPRX2) conjugated to an immunogenic carrier protein was used as the antigen.
• Specificity: MRGPRX2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRGPRX2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 : 200 - 1 : 2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1 : 200 - 1 : 2000
  • Knockdown Validated
  • Single Cell Western
• Application Notes: Use in ICC/IF reported in secitific publication PMID: 32391014
• Control:

  MRGX2 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (Alkanfari IS et al).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.

Alternate Names for MRGX2 Antibody

• MAS-related GPR, member X2
• mas-related G-protein coupled receptor member X2
• MRGPRX2
• MRGX2
• MRGX2G protein-coupled receptor MRGX2

Background

FUNCTION: Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14 seems to be a high potency ligand at this receptor. Cortistatin has several biological functions including roles in sleep regulation locomotor activity, and cortical function. In receptor-expressing cells, cortistatin-stimulated increases in intracellular Ca(2+) but had no effect on basal or forskolin-stimulated cAMP levels, suggesting that this receptor is G(q)-coupled. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Has a limited expression profile, both peripheral and within the central nervous system, with highest levels in dorsal root ganglion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MRGX2 Antibody (NB110-75035)(6)

Publications using NB110-75035

• Subramanian H, Gupta K, Lee D et al. beta-Defensins Activate Human Mast Cells via Mas-Related Gene X2. J Immunol 2013 Jul 1 [PMID: 23698749] (WB, Human)
• Idahosa, CN The Mast Cell Receptor Mas-Related Gene X2 (MrgX2) in Health and Disease. Thesis. 2015 Nov 01 (IHC-P, ICC/IF, Human)
• Alkanfari IS The Role of MRGPRX2/Mrgprb2 in Regulating Mast Cell Function Thesis (Flow, WB, KD, Mouse, Human)
• Callahan BN, Kammala AK, Syed M et al. Osthole, a Natural Plant Derivative Inhibits MRGPRX2 Induced Mast Cell Responses Front Immunol Apr 24 2020 [PMID: 32391014] (ICC/IF, Human)
  
  Details:
  Confocal microscopy
• Alkanfari, I;Freeman, KB;Roy, S;Jahan, T;Scott, RW;Ali, H; Small-Molecule Host-Defense Peptide Mimetic Antibacterial and Antifungal Agents Activate Human and Mouse Mast Cells via Mas-Related GPCRs Cells Apr 3 2019 [PMID: 30987258] (WB, Human)
• Roy S, Ganguly A, Haque M, Ali H. Angiogenic Host Defense Peptide AG-30/5C and Bradykinin B2 Receptor Antagonist Icatibant Are G Protein Biased Agonists for MRGPRX2 in Mast Cells. J. Immunol. Jan 16 2019 [PMID: 30651343] (WB, Human)

Bioinformatics

• Gene Symbol: MRGPRX2
• Entrez:
  • 117194(Human)
• Uniprot:
  • Q96LB1()