Caspase-9 Antibody


Western Blot: Caspase-9 Antibody [NB100-56119] - Analysis of Caspase-9. Various tissue lysates were prepared from human autospsy material and normalized for total protein. Most tissues contained the ~50 kDa more
Immunohistochemistry: Caspase-9 Antibody [NB100-56119] - Analysis of AB expression in formalin-fixed, paraffin-embedded mouse brain tissue sections at 1:2000. Hematoxylin-eosin counterstain. A:Brain striatum, B: Brain more
Simple Western: Caspase-9 Antibody [NB100-56119] - Simple Western lane view shows a specific band for Caspase 9 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa more

Product Details

Reactivity Hu, Mu, Rt, Ca, GeSpecies Glossary
Applications WB, Simple Western, IHC, IHC-Fr, IHC-P, IP
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Caspase-9 Antibody Summary

Recombinant full-length human Caspase-9 protein (pro-form) was used as an immunogen (NP_001220).
This polyclonal antisera recognizes the proform of caspase-9 (~50 kDa), and the large (~35 kDa) and small (~15 kDa) subunits of active/cleaved Caspase-9. Intermediate caspase-9 cleavage forms may also be seen at ~21 kDa.
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
50 ul neat serum
0.05% Sodium Azide
This product is unpurified. The exact concentration of antibody is not quantifiable.


Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
46.3 kDa
Read Publications using
NB100-56119 in the following applications:

  • 1 publication
  • WB
    2 publications

Reactivity Notes

Cross reacts with Dog, Gerbil, Human, Mouse and Rat.

Alternate Names for Caspase-9 Antibody

  • apoptotic protease activating factor 3
  • Apoptotic protease Mch-6
  • Apoptotic protease-activating factor 3
  • Cas9
  • CASP9
  • CASP-9
  • caspase 9, apoptosis-related cysteine peptidase
  • Caspase9
  • caspase-9
  • EC
  • ICE-like apoptotic protease 6
  • MCH6apoptosis-related cysteine protease


Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Caspase-9 polyclonal antisera recognizes the proform of caspase-9 (approx. 50 kDa), and the large (approx. 35 kDa) and small (approx. 15 kDa) subunits of active/cleaved Caspase-9.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-9 Antibody (NB100-56119)(5)

Reviews for Caspase-9 Antibody (NB100-56119) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Caspase-9 Antibody (NB100-56119). (Showing 1 - 1 of 1 FAQs).

  1. Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?
    • After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

Secondary Antibodies


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Blogs on Caspase-9.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol CASP9

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