Caspase-3 Antibody

Images

 
Immunohistochemistry-Paraffin: Caspase-3 Antibody [NB600-1235] - Human tonsil.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Mk, Rb, ShSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Concentration
1.0 mg/ml

Order Details

Caspase-3 Antibody Summary

Immunogen
Recombinant full length protein (Human).
Localization
Cytoplasmic and some nuclear.
Specificity
This reacts with the active form of Caspase 3 (17 kDa protein).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP3
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Conjugate
Unconjugated
Buffer
PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgG purified

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
IHC (formalin/paraffin): staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 (30 min at RT). Use in ICC/IF was reported in the scientific literature (PMID: 23813946).
Publications
Read Publications using
NB600-1235 in the following applications:

Alternate Names for Caspase-3 Antibody

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC 3.4.22.56
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA

Background

Caspase 3 is a member of the interleukin-1 BETA-converting enzyme family. Caspase 3 is thought to be associated with induction of apoptosis.Caspase 3 is ubiquitously expressed and like other caspases is synthesized as an inactive (32kDa) proenzyme. Upon activation, Caspase 3 is cleaved at Asp28-Ser29 and Asp175-Ser176 thereby generating two subunits of 17kDa and 12kDa, respectively. Recent studies have implicated that Caspase 3 is associated with the induction of apoptosis. Activation of Caspase 3 occurs in response to variety of apoptotic inducers including Fas mediated apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56098
Species: Hu, Mu, Ca
Applications: WB, IHC, IHC-P, IP
NB100-56118
Species: Hu, Mu, Rt, Ca, Ge
Applications: IHC, IHC-Fr, IHC-P, IP, Flow-IC
NB100-56096
Species: Hu, Mu, Rt, Ge
Applications: WB, IHC, IHC-Fr, IHC-P, IP
NB100-56599
Species: Hu
Applications: WB, Flow
NB100-56116
Species: Hu, Mu, Rt, Ge
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-56503
Species: Hu, Mu, Rt, Po, Ca, Dr, Eq, Ma, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-56104
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-Fr, IHC-P, IP
NBP2-43728
Species: Hu
Applications: WB, ICC/IF
MAB1489
Species: Hu
Applications: WB
MAB2055
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC, ICFlow
NBP1-47842
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-1925
Species: Ce, Hu(-), Ma(-), Mu(-), Rt(-)
Applications: WB, ELISA, IHC, IHC-P
NB100-56183
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
MAB18251
Species: Hu, Mu
Applications: WB, Simple Western
NB600-1235
Species: Hu, Mu, Rt, Po, Bv, Ca, Mk, Rb, Sh
Applications: ICC/IF, IHC, IHC-P

Publications for Caspase-3 Antibody (NB600-1235)(11)

We have publications tested in 4 confirmed species: Human, Rat, Bovine, Rabbit.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-P.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-P
(6)
All Applications
Filter By Species
Human
(1)
Rat
(6)
Bovine
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB600-1235 Applications Species
Aksu EH, Kandemir FM, Altun S et al. Ameliorative Effect of Carvacrol on Cisplatin-Induced Reproductive Damage in Male Rats. J. Biochem. Mol. Toxicol. 2016 Apr 25 [PMID:27111473] (IHC-P, Rat) IHC-P Rat
Aksu EH, Ozkaraca M, Kandemir FM et al. Mitigation of paracetamol-induced reproductive damage by chrysin in male rats via reducing oxidative stress Andrologia Feb 24 2016 12:00AM [PMID:26914515] (IHC-P, Rat) IHC-P Rat
Hunziker EB, Lippuner K, Keel MJ et al. A novel organ-slice culturing system to simulate meniscal repair: Proof of concept using a synovium-based pool of meniscoprogenitor cells. J. Orthop. Res. 2016 Jan 21 [PMID:26790377] (IHC-P, Human) IHC-P Human
de Souza PC, Balasubramanian K, Njoku C et al. OKN-007 decreases tumor necrosis and tumor cell proliferation and increases apoptosis in a preclinical F98 rat glioma model J Magn Reson Imaging 2015 Apr 29 [PMID:25920494] (IHC, Rat) IHC Rat
Kandemir FM, Ozkaraca M, Yildirim BA et al. Rutin attenuates gentamicin-induced renal damage by reducing oxidative stress, inflammation, apoptosis, and autophagy in rats. Ren Fail 2015 Apr [PMID:25613739] (Rat) Rat
Buyuklu M, Kandemir F, Ozkaraca M et al. Benefical effects of lycopene against contrast medium-induced oxidative stress, inflammation, autophagy, and apoptosis in rat kidney. Hum Exp Toxicol. 2014 Sep 08 [PMID:25205736]
Buyuklu M, Mehmet Kandemir F, Ozkaraca M et al. Protective effect of curcumin against contrast induced nephropathy in rat kidney: what is happening to oxidative stress, inflammation, autophagy and apoptosis? Eur Rev Med Pharmacol Sci 3/10/2014 [PMID:24610611] (IHC-P, Rat) IHC-P Rat
Topcu I, Vatansever S, Bayram E et al. The effects of lornoxicam on neuroprotection following diffuse traumatic brain injury in rats. Turk Neurosurg 2013 [PMID:24310460] (IHC-P, Rat) IHC-P Rat
Wang L, Wu Y, Tan Y et al. Cytotoxic effects of the quinolone levofloxacin on rabbit meniscus cells. J Appl Toxicol 2013 Jul 1 [PMID:23813946] (ICC/IF, Rabbit) ICC/IF Rabbit
Jee D, Lee WK. Inhibitory Effect of intravitreal Injection of Bevacizumab on Nerve Growth Factor. Current Eye Research. 2011 Oct 31. [PMID:22040304]
Show All 11 Publications.

Reviews for Caspase-3 Antibody (NB600-1235) (0)

There are no reviews for Caspase-3 Antibody (NB600-1235). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Caspase-3 Antibody (NB600-1235). (Showing 1 - 4 of 4 FAQs).

  1. Any reason for cleaved caspase 3 to be seen as purely nuclear staining or is this just an artifact?
    • Caspase 3 can translocate from the cytoplasm to the nucleus, usually during apoptosis. We do offer a 100% guarantee that this antibody will be useful as stated on the datasheet also.
  2. The datasheet says that it reacts with the active form of Caspase 3 (17 kDa protein), but every time when I do the WB, I get two bands between 15kDa and 20kDa. The different company's cleaved caspase 3 antibody reacts with 17 and 19 kDa protein, so I wonder which band is specific to yours.
    • Regarding your question, NB600-1235 has been exclusively validated for IHC, not western blot and it is supposed to detect the active form of caspase3. The inactive Caspase 3 is a 31 kD protein, a heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12). When it is Cleaved by granzyme B, it generates the two active subunits. As a result the two bands you are observing on the gel should be correct. However, we haven't tested this antibody for western blot.
  3. I'd like to get the information about the active/cleaved Caspase 3 Antibody (NB600-1235). The datasheet says that it reacts with the active form of Caspase 3 (17 kDa protein), but every time when I do the WB, I get two bands between 15kDa and 20kDa. The different company's cleaved caspase 3 antibody reacts with 17 and 19 kDa protein, so I wonder which band is specific to yours?
    • According to the data I could find for the active/cleaved caspase 3, the active form should appear at approximately 17kDa and also has a smaller subunit seen at approximately 12kDa. The inactive form is approximately 32kDa. These are the theoretical weights and can vary based on type of gel and samples they are run on. In most cases, it looks like most antibodies detect the active forms at 19 and 17kDa respectively. With this particular product, NB600-1235, we have not yet validated it for use in western blot, so we unfortunately do not have any information for the location of bands.
  4. This is a Rabbit antibody right so Do you think I can use it on rabbit tissue?
    • This antibody does react to rabbit but if you are using it with an anti-rabbit secondary, you will almost certainly see some background staining. I know that mouse-on-mouse blocking systems exist, but I am not sure if there is a rabbit equivalent at this time. The best way to use NB600-1235 on rabbit tissues would be to have it directly conjugated to biotin, HRP, or whatever detection method you are planning to use. This will eliminate the need for an anti-rabbit secondary and should give you little to no background. We do offer a conjugation service, so if you would like to have this antibody conjugated, please let me know what you would like to have this antibody conjugated to and I can get you a quote, email bulk@novusbio.com.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Caspase-3 Antibody Products

Related Products by Gene

Bioinformatics Tool for Caspase-3 Antibody (NB600-1235)

Discover related pathways, diseases and genes to Caspase-3 Antibody (NB600-1235). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Caspase-3 Antibody (NB600-1235)

Discover more about diseases related to Caspase-3 Antibody (NB600-1235).
 

Pathways for Caspase-3 Antibody (NB600-1235)

View related products by pathway.

PTMs for Caspase-3 Antibody (NB600-1235)

Learn more about PTMs related to Caspase-3 Antibody (NB600-1235).

Blogs on Caspase-3.

Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

D4-GDI (GDP dissociation inhibitor, RhoGD12)
The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M...  Read full blog post.

LC3B - a novel marker for autophagosome
Autophagy, also known as macroautophagy, supplies alternative fuel for cells that are under environmental stress conditions (including starvation, growth factor deprivation, and hypoxia). This highly regulated and catabolic cell process recycles a...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol CASP3
Entrez
Uniprot

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB600-1235 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought