Caspase-3 Antibody (31A1067) - (Pro and Active), (Azide Free)

Images

 
Western Blot: Caspase-3 (Pro and Active) Antibody (31A1067) [Azide Free] [NBP2-33244] - Lanes 1, 2 and 3 demonstrate the species cross-reactivity of the antibody in human, mouse and rat heart lysate, respectively.
Immunohistochemistry-Paraffin: Caspase-3 (Pro and Active) Antibody (31A1067) [Azide Free] [NBP2-33244] - Analysis in the human thymus after heat-induced antigen retrieval at 5 ug/ml.
Western Blot: Caspase-3 (Pro and Active) Antibody (31A1067) [Azide Free] [NBP2-33244] - Analysis of Caspase-3 in A) RAW and B) NIH 3T3 using 32 ug/ml.
Western Blot: Caspase-3 (Pro and Active) Antibody (31A1067) [Azide Free] [NBP2-33244] - Analysis for detection of Caspase-3 activation in HeLa cells. Cells were treated with 2mM staurosporine for different time periods. ...read more
Western Blot: Caspase-3 (Pro and Active) Antibody (31A1067) [Azide Free] [NBP2-33244] - Western blot of Caspase-3 in multiple human tissues. The tissues shown are A) brain, B) heart, C) intestine, D) kidney, E) liver, ...read more
Immunohistochemistry-Paraffin: Caspase-3 (Pro and Active) Antibody (31A1067) [Azide Free] [NBP2-33244] - Analysis in human breast cancer stained at 4ug/ml. Localization can be cytoplasmic and nuclear. Staining in the ...read more
Immunohistochemistry-Paraffin: Caspase-3 (Pro and Active) Antibody (31A1067) [Azide Free] [NBP2-33244] - Analysis in normal colon stained 4ug/ml. Localization can be cytoplasmic and nuclear. Staining in the nucleus is ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, ChHa, MaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
31A1067
Clonality
Monoclonal
Host
Mouse

Caspase-3 Antibody (31A1067) - (Pro and Active), (Azide Free) Summary

Immunogen
Full-length recombinant human caspase-3 protein was used as immunogen. The antibody recognizes an epitope in the large domain subunit of Caspase-3. As such it will recognize pro Caspase-3 and the large subunit cleavage fragment.
Specificity
The antibody detects both pro Caspase-3 (~32 kDa) and the large subunit of the active/cleaved form (~14-21 kDa) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. It is highly recommended that a maximum sensitivity ECL substrate (Femto sensitive) be used for efficient detection of this antibody in Western blot applications.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CASP3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS - BSA free
Preservative
No Preservative
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 1-5 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Useful in Immunohistochemistry-Frozen See Zhang et al., and Immunohistochemistry-Paraffin See Lee et al. Use in Immunocytochemistry/Immunofluorescence was reported in the scientific literature (PMID: 23840553).

Alternate Names for Caspase-3 Antibody (31A1067) - (Pro and Active), (Azide Free)

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC 3.4.22.56
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Caspase-3- A marker of programmed cell death
Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environmental cues and cellul...  Read full blog post.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
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Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

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LC3B - a novel marker for autophagosome
Autophagy, also known as macroautophagy, supplies alternative fuel for cells that are under environmental stress conditions (including starvation, growth factor deprivation, and hypoxia). This highly regulated and catabolic cell process recycles a...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol CASP3
Entrez
Uniprot

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