Genetic Strategies: Knockout Validated: Caspase-1 Antibody [NB100-56564] - Human epidermoid carcinoma parental cell line and Caspase-1 knockout (KO) A431 cell line. PVDF membrane was probed with 0.5 ug/ml of ...read more
Western Blot: Caspase-1 Antibody [NB100-56564] - Analysis of Caspase-1 using a polyclonal antibody. Human HeLa lysate probed with Caspase-1 antibody at 0.5 ug/ml.
Immunohistochemistry-Paraffin: Caspase-1 Antibody [NB100-56564] - Human reactive lymph node stained for Caspase-1 expression (NB100-56564) using a DAB chromogen and Hematoxylin counterstain. B is a higher magnification ...read more
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis (1). The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a tetramer to form the active enzyme. Caspase-1/ICE (IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon-gamma-inducing factor.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Caspase-1 Antibody (NB100-56564). (Showing 1 - 2 of 2 FAQ).
We are interested in your item, NB100-56564 (IMG-5025) and would like to confirm if it only recognizes subunit p10 and do not cross react to p20?
Our Caspase 1 antibody with catalogue number NB100-56564 (IMG-5025) was raised against a synthetic peptide corresponding to amino acids 371-390 of the human protein. According to the Uniprot listing these amino acids lie within the p10 subunit. The antibody recognises full-length Caspase-1, as well as cleaved Caspase-1 forms that include amino acids 31-45. Amino acids 31-45 lie within the propeptide. Unfortunately we have no testing data where we have used this antibody to probe the isolated p20 subunit. Based on the immunogen sequence it seems unlikely that the antibody would recognise p20, but I unfortunately do not have 100% certainty on this at the present time.
We are interested in your items, NB100-56565 and NB100-56564 and I would like to use them for recognizing the cleaved form of caspase 1. Would you please let me know if NB100-56565 and NB100-56564 recognize the caspase-1 p10 or p20 ? If so, could you please provide me any supporting data of them?
For NB100-56565 & NB100-56564 it will recognize the full length form and the p10 as the immunogen. A synthetic peptide corresponding to amino acids 371-390 RKVRFSFEQPDGRAQMPTTE of human caspase-1 was used as immunogen. The p10 subunit is aa 307-404. If you need an antibody to the p20 subunit:http://www.novusbio.com/Caspase-1-Antibody_NBP1-45433.html. This will bind to the the region around aa 132. The p20 subunit is aa 120-297.
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