CARD8 Antibody

Images

 
Western Blot: CARD8 Antibody [NB100-56181] - Analysis of CARD8 in tumor cell lines using this antibody. Cell lysates were normalized for total protein content.
Immunohistochemistry-Paraffin: CARD8 Antibody [NB100-56181] - Formalin-fixed, paraffin-embedded tumor/normal adjacent tissue cores from a human colon tissue microarray stained for TUCAN/CARD8 expression at 1:2000 using ...read more
Immunohistochemistry-Paraffin: CARD8 Antibody [NB100-56181] - Formalin-fixed, paraffin-embedded tissue human colon microarray stained for TUCAN/CARD8 expression at 1:2000 using NB100-56181. A-A2, sucessively higher ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CARD8 Antibody Summary

Immunogen
Full-length recombinant human TUCAN (CARD8) protein was used as immunogen. The recombinant protein sequence corresponds to GenBank no. gi|14424229|sp|Q9Y2G2|CARD8_HUMAN.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CARD8
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
Application Notes
The gene encoding TUCAN produces, by alternative splicing, multiple transcripts, with introns, putatively encoding numerous different protein isoforms. Thus the molecular weight observed by SDS-PAGE can vary depending on the TUCAN form expressed.
Publications
Read Publication using
NB100-56181 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for CARD8 Antibody

  • Apoptotic protein NDPP1
  • CARD inhibitor of NF-kappaB-activating ligands
  • CARDINALFLJ18121
  • CARD-inhibitor of NF-kappa-B-activating ligand
  • caspase recruitment domain family, member 8
  • caspase recruitment domain-containing protein 8
  • DACAR
  • DAKAR
  • KIAA0955DKFZp779L0366
  • NDPP
  • NDPP1MGC57162
  • TUCANFLJ18119
  • Tumor up-regulated CARD-containing antagonist of CASP9
  • tumor up-regulated CARD-containing antagonist of caspase nine

Background

TUCAN (also known as CARD8 and CARDINAL) is a CARD domain containing protein. TUCAN stands for tumor-up-regulated CARD-containing antagonist of caspase nine. Proteins containing a CARD (caspase-associated recruitment domain) domain are key regulators of cell death, cell survival and cytokine production (reviewed in Damiano and Reed, 2004). CARD domains are found in the N-terminal pro-domains of certain caspases, a family of apoptotic and pro-inflammatory proteases, as well as in a diversity of other proteins including TUCAN. CARD domains are homotypic protein interaction motifs that enable networks of proteins to communicate via CARD-CARD interactions. TUCAN is an anti-apoptotic CARD protein that can protect tumors from cell death stimuli, and is overexpressed in certain forms of cancer. The role of TUCAN in tumor biology remains to be fully elucidated, and there appears to be be various mechanisms by TUCAN can potentially contribute to apoptosis resistance (reviewed in Checinska et al, 2006). For example, TUCAN has been shown to inhibit caspase-9 activation by binding to the CARD region of pro-caspase-9, thereby suppressing the formation of the Apaf-1-caspase-9 apoptotic complex and apoptosis. Additionally, a TUCAN isoform has been described that blocks both caspase-8 and caspase-9 mediated apoptosis. However, in some tumors, TUCAN does not appear to interact with caspase-9 and may play a role in modulating NF-kB transcription factor survivial signaling pathways. In this regard a CARD-independent interaction of TUCAN with Ikkg has been described, resulting in the inhibition of interleukin-1 and TNF-induced NF-kB activation. Human TUCAN is a 431 amino acid protein according to GenBank no. gi|14424229|sp|Q9Y2G2|CARD8_HUMAN, and migrates at ~45-49 kDa on SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CARD8 Antibody (NB100-56181)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol CARD8
Entrez