Carbonic Anhydrase XII/CA12 Antibody (CL0280) - BSA Free

Western Blot: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902] - Lane 1: Marker [kDa], Lane 2: Human RT-4 cell line

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902] - Staining of human pancreas shows strong membranous and moderate cytopalsmic positivity in the exocrine cells.

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902] - Staining of human colon shows strong cytoplasmic and membrane positivity in glandular cells.

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902] - Staining of human kidney (renal cancer) shows moderate immunoreactivity in cancer cells.

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902] - Staining of human kidney shows strong membranous and/or moderate cytoplasmic immunoreactivity in subsets of renal tubules, while glomeruli are negative.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL0280
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Carbonic Anhydrase XII/CA12 Antibody (CL0280) - BSA Free Summary

• Description: Novus Biologicals Mouse Carbonic Anhydrase XII/CA12 Antibody (CL0280) - BSA Free (NBP2-52902) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from O43570, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CA12
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Carbonic Anhydrase XII/CA12 Recombinant Protein Antigen (NBP2-52902PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Carbonic Anhydrase XII/CA12 Antibody (CL0280) - BSA Free

• CA12
• Carbonate dehydratase XII
• carbonic anhydrase 12
• Carbonic Anhydrase XII
• carbonic anhydrase XIICAXII
• carbonic dehydratase
• CA-XII
• EC 4.2.1.1
• FLJ20151
• HsT18816
• Tumor antigen HOM-RCC-3.1.3

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CA12