Carbonic Anhydrase IV/CA4 Antibody [Unconjugated]

Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Carbonic Anhydrase IV/CA4 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysates of mouse lung tissue and mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 51-52 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Mu
• Applications: WB, Simple Western, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Carbonic Anhydrase IV/CA4 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IV/CA4
  Glu18-Ser277
  Accession # Q64444
• Specificity: Detects mouse Carbonic Anhydrase IV/CA4 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human CA4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Car4

Applications/Dilutions

• Dilutions:
  • Western Blot 0.25 ug/mL
  • Simple Western 12.5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase IV/CA4 Antibody [Unconjugated]

• CA4
• CAIV
• CA-IV
• Car4
• Carbonate dehydratase IV
• carbonic anhydrase 4
• Carbonic Anhydrase IV
• carbonic anhydrase IVRP17
• carbonic dehydratase IV
• EC 4.2.1.1
• retinitis pigmentosa 17 (autosomal dominant)
• RP17

Background

Carbonic anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. The deduced amino acid sequence of mouse CA4 consists of a signal peptide (residues 1‑17), an ectodomain (residues 18‑277) and a pro peptide (residues 278‑305), which is removed in the mature form (2). The native enzyme is attached to the membrane by a GPI‑anchor. Recombinant mouse CA4 corresponds to the ectodomain and exhibits the activity as described in Activity Assay Protocol.

1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
2. Tamai, S. et al. (1996) Biochem. Genet. 34:31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Carbonic Anhydrase IV/CA4 Antibody (AF2414)(12)

Publications using AF2414

• A Ohishi, K Nishida, K Miyamoto, M Imai, R Nakanishi, K Kobayashi, A Hayashi, K Nagasawa Bortezomib alters sour taste sensitivity in mice Toxicol Rep, 2017;4(0):172-180. 2017 [PMID: 28959638] (IHC-Fr, Mouse)
• H Lee, LJ Macpherson, CA Parada, CS Zuker, NJP Ryba Rewiring the taste system Nature, 2017;548(7667):330-333. 2017 [PMID: 28792937] (IHC, Mouse)
• W Ren, E Aihara, W Lei, N Gheewala, H Uchiyama, RF Margolskee, K Iwatsuki, P Jiang Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation Sci Rep, 2017;7(1):4004. 2017 [PMID: 28638111] (ICC, Mouse)
• D Zocchi, G Wennemuth, Y Oka The cellular mechanism for water detection in the mammalian taste system Nat. Neurosci., 2017;0(0):. 2017 [PMID: 28553944] (IHC, Mouse)
• Yugo Nishiguchi Bcl11b/Ctip2 is required for development of lingual papillae in mice Dev Biol, 2016;0(0):. 2016 [PMID: 27287879] (IHC, Mouse)
• Wandernoth P, Mannowetz N, Szczyrba J, Grannemann L, Wolf A, Becker H, Sly W, Wennemuth G Normal Fertility Requires the Expression of Carbonic Anhydrases II and IV in Sperm. J Biol Chem, 2015;290(49):29202-16. 2015 [PMID: 26487715] (IHC-P, Mouse)
• Castillo D, Seidel K, Salcedo E, Ahn C, de Sauvage F, Klein O, Barlow L Induction of ectopic taste buds by SHH reveals the competency and plasticity of adult lingual epithelium. Development, 2014;141(15):2993-3002. 2014 [PMID: 24993944] (IHC-Fr, Mouse)
• Wen T, Mingler M, Wahl B, Khorki M, Pabst O, Zimmermann N, Rothenberg M Carbonic anhydrase IV is expressed on IL-5-activated murine eosinophils. J Immunol, 2014;192(12):5481-9. 2014 [PMID: 24808371] (Flow Cytometry, Mouse)
• Reibring C, El Shahawy M, Hallberg K, Kannius-Janson M, Nilsson J, Parkkila S, Sly W, Waheed A, Linde A, Gritli-Linde A Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation. PLoS ONE, 2014;9(5):e96007. 2014 [PMID: 24789143]
• Kim A, Feng P, Ohkuri T, Sauers D, Cohn ZJ, Chai J, Nelson T, Bachmanov AA, Huang L, Wang H Defects in the peripheral taste structure and function in the MRL/lpr mouse model of autoimmune disease. PLoS ONE, 2012;7(4):e35588. 2012 [PMID: 22536412] (IHC-Fr, Mouse)
• Akiba Y, Mizumori M, Kuo M, Ham M, Guth PH, Engel E, Kaunitz JD CO2 chemosensing in rat oesophagus. Gut, 2008;57(12):1654-64. 2008 [PMID: 18682519] (ICC, Rat)
• Akiba Y, Ghayouri S, Takeuchi T, Mizumori M, Guth PH, Engel E, Swenson ER, Kaunitz JD Carbonic anhydrases and mucosal vanilloid receptors help mediate the hyperemic response to luminal CO2 in rat duodenum. Gastroenterology, 2006;131(1):142-52. 2006 [PMID: 16831598] (IHC, Rat)

Bioinformatics

• Gene Symbol: Car4
• Uniprot:
  • Q64444()