Calreticulin Antibody

Images

 
Western Blot: Calreticulin Antibody [NB600-103] - Detection of calreticulin in human kidney lysate using NB 600-103 at 1:1,000. ECL exposure, 10 seconds.
Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody [NB600-103] - IF Confocal analysis of 3T3 cells using Calreticulin antibody (NB600-103, 1:20). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as ...read more
Immunohistochemistry-Paraffin: Calreticulin Antibody [NB600-103] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human prostate cancer using Calreticulin antibody at 1:20 on a Bond Rx autostainer (Leica ...read more
Western Blot: Calreticulin Antibody [NB600-103] - Western blot analysis of HeLa and C6 cell lysate using calreticulin antibody at 1:1000.
Simple Western: Calreticulin Antibody [NB600-103] - Simple Western lane view shows a specific band for Calreticulin in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Calreticulin Antibody Summary

Immunogen
A full-length protein to mouse Calreticulin [UniProt# P14211]
Localization
Calreticulin staining is localized to the ER in most cell types and under most conditions.
Marker
Endoplasmic Reticulum Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CALR
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Simple Western 1:500
  • Immunocytochemistry/Immunofluorescence 1:20-1:100
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:20
Application Notes
This Calreticulin antibody is useful for Western blot, where a band at approx. 55 kDa is seen. Note that Calreticulin has several reported isoforms that range from 48-62 kDa. ICC data from customer review. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 26643212).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Calreticulin Lysate (NBL1-08655)
Reviewed Applications
Read 2 Reviews rated 5
using
NB600-103 in the following applications:

Publications
Read Publications using
NB600-103 in the following applications:

Reactivity Notes

Human and Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for Calreticulin Antibody

  • Autoantigen Ro
  • CALR
  • Calregulin
  • Calreticulin
  • cC1qR
  • CRP55
  • CRT
  • CRTC
  • Endoplasmic reticulum resident protein 60
  • ERp60
  • FLJ26680
  • grp60
  • RO
  • Sicca syndrome antigen A (autoantigen Ro; calreticulin)
  • SSA
  • SSAHACBP
  • Vasostatin

Background

Calreticulin (CRT) is a ubiquitous multifunctional calcium-binding protein which attaches to the surface of cells and implicates in signal transduction events associated with innate immunity, cell adhesion, and apoptosis. Because CRT lacks a trans-membrane domain, CD91 (LDLR-related protein 1) has been proposed as a candidate anchoring receptor for this protein. CRT is mainly localized in ER lumen, cytoplasmic granules as well as in sarcoplasmic reticulum lumen as a monomer where it forms a component of an EIF2 complex (CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5) and interacts with GABARAP, NR3C1, PDIA3/ERp57, TRIM21 etc. CRT is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in ER via the calreticulin/calnexin cycle. It interacts with DNA-binding domain of NR3C1 for facilitating its nuclear export. CRT is involved in maternal gene expression regulation and participates in oocyte maturation via regulation of calcium homeostasis. In immune system, cell surface CRT (csCRT) serves as the signal-transducing receptor for collectin family proteins which binds to foreign organisms or apoptotic cells through their globular heads, while their collagen-like tails bind to csCRT, which leads to phagocytosis. CRT located in the cytoplasm, at the cell membrane and extracellular matrix functions in many physiological processes, such as cell adhesion, phagocytosis, and tumor recognition.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-103
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Calreticulin Antibody (NB600-103)(4)

Reviews for Calreticulin Antibody (NB600-103) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 2 application: ICC, WB.

Reviews using NB600-103:
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ICC
(1)
WB
(1)
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Human
(1)
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Calreticulin NB600-103
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reviewed by:
Anony- mous
ICC Mouse 06/11/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesMouse
Special ApplicationsIF Confocal analysis of 3T3 cells using Calreticulin antibody (NB600-103, 1:20). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF
Western Blot Calreticulin NB600-103
Enlarge
reviewed by:
Anony- mous
WB Human 06/11/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsWestern blot analysis of extracts from HeLa and C6 cells using Calreticulin antibody (NB600-103, 1:1000).
FileView PDF

Product General Protocols

View specific protocols for Calreticulin Antibody (NB600-103): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calreticulin Antibody (NB600-103) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Calreticulin Antibody (NB600-103)

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Research Areas for Calreticulin Antibody (NB600-103)

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Blogs on Calreticulin.

Calreticulin - ER chaperone involved in calcium homeostasis and protein quality control
Calreticulin is a calcium-dependent ER chaperone, involved in protein folding, maturation, and cellular localization. Calreticulin is a highly conserved 48 kDa protein encoded by the CALR gene. Calreticulin and its homolog calnexin regulate the fo...  Read full blog post.

Calnexin - an ER chaperone that folds the cell's glycoproteins
Calnexin is an abundant 90kDa chaperone protein that resides in the membrane of the endoplasmic reticulum. Calnexin and the related calreticulin protein function together to ensure the proper folding of glycoproteins. By binding to partially folded...  Read full blog post.

Calreticulin: a Multiprocess Calcium Buffering Chaperone
Calreticulin is a Calcium binding chaperone that has multiple functions both inside and outside the endoplasmic reticulum. Calreticulin is involved in the quality control of newly synthesized proteins and glycoproteins, interacting with various other...  Read full blog post.

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Recent Reviews

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5
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2
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1
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Anony- mous
06/11/2014
Application: ICC
Species: Mouse

Anony- mous
06/11/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol CALR
Entrez
Uniprot