Calreticulin Antibody


Western Blot: Calreticulin Antibody [NB600-103] - Detection of calreticulin in human kidney lysate using NB 600-103 at 1:1,000. ECL exposure, 10 seconds.
Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody [NB600-103] - IF Confocal analysis of 3T3 cells using Calreticulin antibody (NB600-103, 1:20). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as more
Western Blot: Calreticulin Antibody [NB600-103] - Western blot analysis of HeLa and C6 cell lysate using calreticulin antibody at 1:1000.
Simple Western: Calreticulin Antibody [NB600-103] - Simple Western lane view shows a specific band for Calreticulin in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC-Fr
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Calreticulin Antibody Summary

A full-length protein to mouse Calreticulin [UniProt# P14211]
Calreticulin staining is localized to the ER in most cell types and under most conditions.
Endoplasmic Reticulum Marker
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.1% Sodium Azide
This product is unpurified. The exact concentration of antibody is not quantifiable.


Application Notes
This Calreticulin antibody is useful for Western blot, where a band at ~55 kDa is seen. Note that Calreticulin has several reported isoforms that range from 48-62 kDa. ICC data from customer review.In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 26643212).
Theoretical MW
55 kDa
Reviewed Applications
Read 2 Reviews rated 5
NB600-103 in the following application:

Read Publications using
NB600-103 in the following applications:

Reactivity Notes

Human and Mouse.

Alternate Names for Calreticulin Antibody

  • Autoantigen Ro
  • CALR
  • Calregulin
  • calreticulin
  • cC1qR
  • CRP55
  • CRT
  • CRTC
  • Endoplasmic reticulum resident protein 60
  • ERp60
  • FLJ26680
  • grp60
  • RO
  • Sicca syndrome antigen A (autoantigen Ro; calreticulin)
  • SSA
  • Vasostatin


Calreticulin (CRT) is a ubiquitous multifunctional calcium-binding protein which attaches to the surface of cells and implicates in signal transduction events associated with innate immunity, cell adhesion, and apoptosis. Because CRT lacks a trans-membrane domain, CD91 (LDLR-related protein 1) has been proposed as a candidate anchoring receptor for this protein. CRT is mainly localized in ER lumen, cytoplasmic granules as well as in sarcoplasmic reticulum lumen as a monomer where it forms a component of an EIF2 complex (CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5) and interacts with GABARAP, NR3C1, PDIA3/ERp57, TRIM21 etc. CRT is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in ER via the calreticulin/calnexin cycle. It interacts with DNA-binding domain of NR3C1 for facilitating its nuclear export. CRT is involved in maternal gene expression regulation and participates in oocyte maturation via regulation of calcium homeostasis. In immune system, cell surface CRT (csCRT) serves as the signal-transducing receptor for collectin family proteins which binds to foreign organisms or apoptotic cells through their globular heads, while their collagen-like tails bind to csCRT, which leads to phagocytosis. CRT located in the cytoplasm, at the cell membrane and extracellular matrix functions in many physiological processes, such as cell adhesion, phagocytosis, and tumor recognition.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calreticulin Antibody (NB600-103)(4)

Reviews for Calreticulin Antibody (NB600-103) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 2 application: ICC, WB.

Reviews using NB600-103:
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Images Ratings Applications Species Date Details
Immunocytochemistry Calreticulin NB600-103
reviewed by:
ICC Mouse 06/11/2014


Special ApplicationsIF Confocal analysis of 3T3 cells using Calreticulin antibody (NB600-103, 1:20). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
FileView PDF
Western Blot Calreticulin NB600-103
reviewed by:
WB Human 06/11/2014


ApplicationWestern Blot
Special ApplicationsWestern blot analysis of extracts from HeLa and C6 cells using Calreticulin antibody (NB600-103, 1:1000).
FileView PDF

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calreticulin Antibody (NB600-103) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on Calreticulin.

Calreticulin - ER chaperone involved in calcium homeostasis and protein quality control
Calreticulin is a calcium-dependent ER chaperone, involved in protein folding, maturation, and cellular localization. Calreticulin is a highly conserved 48 kDa protein encoded by the CALR gene. Calreticulin and its homolog calnexin regulate the fo...  Read full blog post.

Calnexin - an ER chaperone that folds the cell's glycoproteins
Calnexin is an abundant 90kDa chaperone protein that resides in the membrane of the endoplasmic reticulum. Calnexin and the related calreticulin protein function together to ensure the proper folding of glycoproteins. By binding to partially folded...  Read full blog post.

Calreticulin: a Multiprocess Calcium Buffering Chaperone
Calreticulin is a Calcium binding chaperone that has multiple functions both inside and outside the endoplasmic reticulum. Calreticulin is involved in the quality control of newly synthesized proteins and glycoproteins, interacting with various other...  Read full blog post.

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Product PDFs


Gene Symbol CALR

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