Cadherin-11 Antibody (1A5) - Azide and BSA Free

Western Blot: Cadherin-11 Antibody (1A5) [H00001009-M04] - Analysis of CDH11 expression in human ovarian cancer.

Flow Cytometry: Cadherin-11 Antibody (1A5) [H00001009-M04] - Analysis of negative control PC3-MM2 cells only, mock control cells staining with 2nd antibodies and CDH11 stained PC3-MM2 cells.

Western Blot: Cadherin-11 Antibody (1A5) [H00001009-M04] - Analysis of full length CDH11 over expression in L cell using CDH11 monoclonal antibody (M03), clone 1A5, and immunoblotted with CDH11 monoclonal antibody (Zymed).

Western Blot: Cadherin-11 Antibody (1A5) [H00001009-M04] - Analysis of CDH11 expression in human kidney.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, Func, IHC, IP
• Clone: 1A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Cadherin-11 Antibody (1A5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CDH11 (P55287, 54 a.a. ~ 612 a.a) partial recombinant protein with Fc tag at C terminal. MW of the Fc tag alone is 50 KDa. 0
• Specificity: This product is specific for Human CDH11 monoclonal antibody (M04), clone 1A5 [Gene ID: 1009].
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH11
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Functional
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
• Application Notes: Functional study: Effective in blocking cadherin-11-mediated cell aggregation.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cadherin-11 Antibody (1A5) - Azide and BSA Free

• CAD11OSF-4
• cadherin 11, type 2, OB-cadherin (osteoblast)
• Cadherin11
• Cadherin-11
• CDH11
• CDHOB
• OB
• OB-Cadherin
• Osteoblast cadherin

Background

Cadherin-11 is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. Cadherin-11 is expressed in osteoblasts and the brain as well as other tissues.Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cadherin-11 Antibody (H00001009-M04)(2)

Publications using H00001009-M04

• Sfikakis PP, Christopoulos PF, Vaiopoulos AG et al. Cadherin-11 mRNA transcripts are frequently found in rheumatoid arthritis peripheral blood and correlate with established polyarthritis. Clin Immunol. 2014-08-27 [PMID: 25173800]
• Lee YC, Bilen MA, Yu G et al. Inhibition of cell adhesion by a cadherin-11 antibody thwarts bone metastasis. Mol Cancer Res 2013-08-02 [PMID: 23913163]

Bioinformatics

• Gene Symbol: CDH11
• Uniprot:
  • P55287(Human)