C-Peptide Antibody (CII-29) - BSA Free

• Reactivity: Mu, Rt
• Applications: ELISA, ICC/IF, IP
• Clone: CII-29
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

C-Peptide Antibody (CII-29) - BSA Free Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with fragments of rat C-peptides I and II conjugated with the carrier protein.
• Specificity: Reacts with rat c-peptide I, rat c-peptide II, mouse c-peptide I, mouse c-peptide II and rat proinsulin.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: INS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunoassay
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Sandwich ELISA 1:100-1:2000
• Application Notes: Can be used for separate or consistent detection of rat C-peptide isoforms (rat C-peptide I and/or rat C-peptide II) in biological samples. The C-Peptide Antibody (CC27) NBP1-05434 can be use as the capture antibody with the use of detection antibody C-Peptide Antibody (CII-29) NBP1-05439 in Sandwich ELISA. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 28988715). Use in IP reported in secitific publication PMID: 32433667

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for C-Peptide Antibody (CII-29) - BSA Free

• C-P
• C-Peptide
• IDDM2
• ILPR
• Insulin C-Peptide
• insulin
• IRDN
• MODY10
• Proinsulin C-Peptide
• proinsulin

Background

C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for C-Peptide Antibody (NBP1-05439)(2)

Publications using NBP1-05439

• Viviano J, Brecker M, Ferrara-Cook C et al. ERp29 as a regulator of Insulin biosynthesis PLoS ONE 2020-05-20 [PMID: 32433667] (IP, Mouse)
• Amatya C, Radichev IA, Ellefson J et al. Self-Transducible Bimodal PDX1-FOXP3 Protein Lifts Insulin Secretion and Curbs Autoimmunity, Boosting Tregs in Type 1 Diabetic Mice. Mol. Ther. 2018-01-03 [PMID: 28988715] (ICC/IF, Rat)

Bioinformatics

• Gene Symbol: INS
• Entrez:
  • 16334(Mouse)
  • 24506(Rat)