c-Maf Antibody

Images

 
Western Blot: c-Maf Antibody [NBP2-24551] - Total protein from Human Pancreas and Human Small Intestine was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human liver cancer using c-MAF antibody at 2ug/ml concentration (1:500 dilution). ...read more
Western Blot: c-Maf Antibody [NBP2-24551] - Analysis of Lysate from human brain in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain probed at 3 ug/ml.
Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - Staining of Human kidney probed with c-maf antibody at 10 ug/ml.
Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - Staining of Human placenta probed with c-maf antibody at 10 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, MkSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

c-Maf Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 75-110 of human c-maf was used as the immunogen, GenBank no. NP_001026974.1|.
Specificity
100% homologous in human isoforms CRA_a, CRA_b, and CRA_c).
Predicted Species
Monkey (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Reviewed Applications
Read 2 Reviews rated 3
using
NBP2-24551 in the following applications:

Publications
Read Publications using
NBP2-24551 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Chimpanzee (87%), Bovine, Equine (81%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1 ml PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for c-Maf Antibody

  • Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene
  • CCA4
  • c-maf proto-oncogene
  • cMaf
  • c-Maf
  • MAF2
  • MGC71685
  • Proto-oncogene c-Maf
  • T lymphocyte c-maf long form
  • transcription factor Maf
  • v-maf musculoaponeurotic fibrosarcoma (avian) oncogene homolog
  • v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian)
  • V-maf musculoaponeurotic fibrosarcoma oncogene homolog

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

6507-IL/CF
Species: Hu
NBP1-32822
Species: Hu, Mu, Rt, Al, Av, Ce, Pl
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, ChIP
NBP2-67471
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB110-75039
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-82580
Species: Hu
Applications: WB, Simple Western, IHC, IHC-P
NBP2-45718
Species: Hu
Applications: WB, Flow, IHC
NBP1-43299
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
H00002551-M03
Species: Hu
Applications: WB, ELISA, ICC/IF, IP
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
AF2605
Species: Hu
Applications: WB, Simple Western, IHC, ICC
H00008131-M02
Species: Hu, Rt
Applications: WB, ELISA
AF2605
Species: Hu
Applications: WB, Simple Western, IHC, ICC
NBP2-32840
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC-P, IP
MAB3809
Species: Hu, Mu
Applications: WB
NB300-750
Species: Hu, Mu, Rt, Fi, Mk, Pm, Sh
Applications: WB, ICC/IF, IP, ICC
Supplier Logo

Publications for c-Maf Antibody (NBP2-24551)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, WB.


Filter By Application
IHC
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species

Reviews for c-Maf Antibody (NBP2-24551) (2) 32

Average Rating: 3
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP2-24551:
Filter by Applications
IHC-P
(1)
ICC
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Human 11/11/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedM1/M2 cell lines
SpeciesHuman
Lot11082976A
Immunocytochemistry c-Maf NBP2-24551
Enlarge
reviewed by:
Natalie Erdmann
ICC Human 07/28/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedMultiple myeloma cells lines MY5 & RPMI-8226 and T cell leukemia cell line Jurkat cytospins
SpeciesHuman
Lot11082976A

Blocking

Blocking Details10% donkey serum in PBS 1 hour at room temperature

Primary Anitbody

Dilution Ratio1/50 or 1/500 1ary antibody diluted in 10% donkey serum/PBS incubated O/N at 4oC.

Secondary Antibody

Secondary DescriptionAlexa Fluor 488 F(ab)2 donkey anti-rabbit IgG (H+L)
Secondary Manufacturer Cat#Jackson 711-546-152
Secondary Concentration1/1000

Details

Detection NotesZeiss AxioImager, FITC filter cube for 400 ms exposure
Fixation DetailsImmersion-fixed in 4% paraformaldehyde 10 minutes, 0.5% Triton X-100 5 minutes permeabilization at room temperature
Wash DescriptionPBS 3X quick washes after each step. PBS 3X 10 minutes before mounting in DAPI/Vectashield.

Comments

CommentsMY5 & RPMI-8226 should have nuclear staining (see Novus #MAB8226) and Jurkat should be negative.Novus notes: This customer tested the product in an untested application that was not listed as a guaranteed application on the datasheet. For this they earn a voucher for 100% of the purchase price as part of our Innovator's Reward Program.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for c-Maf Antibody (NBP2-24551) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for c-Maf Antibody (NBP2-24551)

Discover related pathways, diseases and genes to c-Maf Antibody (NBP2-24551). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for c-Maf Antibody (NBP2-24551)

Discover more about diseases related to c-Maf Antibody (NBP2-24551).
 

Pathways for c-Maf Antibody (NBP2-24551)

View related products by pathway.

PTMs for c-Maf Antibody (NBP2-24551)

Learn more about PTMs related to c-Maf Antibody (NBP2-24551).

Blogs on c-Maf

There are no specific blogs for c-Maf, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

3
5
1
4
0
3
0
2
0
1
1

 
Anonymous
11/11/2015
Application: IHC-P
Species: Human

Natalie Erdmann
07/28/2015
Application: ICC
Species: Human

Bioinformatics

Gene Symbol MAF
Uniprot