c-Maf Antibody - BSA Free

Western Blot: c-Maf Antibody [NBP2-24551] - Total protein from Human Pancreas and Human Small Intestine was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-cMaf in 1% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Western Blot: c-Maf Antibody [NBP2-24551] - SUM159 and HCC1954 whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. c-Maf Antibody (NBP2-24551) was used for primary antibody: 1:500, 4C, overnight. WB image submitted by a verified customer review.

Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human liver cancer using c-MAF antibody at 2ug/ml concentration (1:500 dilution). The primary antibody binding to c-MAF protein was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This c-MAF antibody showed a very strong nuclear and moderate to strong staining in the endothelial cells (blood vessels) and in a subset of apparently infiltrating inflammatory cells. Weak to moderate cytoplasmic and nuclear staining was observed in liver cancer cells and the cells of tumor stroma including fibroblasts.

Western Blot: c-Maf Antibody [NBP2-24551] - Analysis of Lysate from human brain in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain probed at 3 ug/ml.

Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - Staining of Human kidney probed with c-maf antibody at 10 ug/ml.

Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - Staining of Human placenta probed with c-maf antibody at 10 ug/ml.

• Reactivity: Hu, Mu, Rt, Bv, Pm
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

c-Maf Antibody - BSA Free Summary

• Immunogen: A synthetic peptide corresponding to amino acids 75-110 of human c-maf was used as the immunogen, GenBank no. NP_001026974.1|.
• Specificity: 100% homologous in human isoforms CRA_a, CRA_b, and CRA_c).
• Predicted Species: Monkey (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 2-4 ug/ml

Reactivity Notes

Chimpanzee (87%), Bovine, Equine (81%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 ml PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for c-Maf Antibody - BSA Free

• Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene
• CCA4
• c-maf proto-oncogene
• cMaf
• c-Maf
• MAF2
• MGC71685
• Proto-oncogene c-Maf
• T lymphocyte c-maf long form
• transcription factor Maf
• v-maf musculoaponeurotic fibrosarcoma (avian) oncogene homolog
• v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian)
• V-maf musculoaponeurotic fibrosarcoma oncogene homolog

Background

c-Maf is part of the Maf family of transcription factors that possess a conserved basic region and a leucine zipper domain that mediate DNA and protein-protein binding and plays an important role in tissue-specific gene regulation and cell differentiation. c-Maf is a transcriptional activator of interleukin 4 and 10 in T-cells, and is involved in lens fiber cell differentiation. c-Maf translocation has been observed in human multiple myeloma and may be oncogenic.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for c-Maf Antibody (NBP2-24551)(2)

Publications using NBP2-24551

• Aoki H, Ogino H, Tomita H et al. Disruption of Rest Leads to the Early Onset of Cataracts with the Aberrant Terminal Differentiation of Lens Fiber Cells. PLoS ONE. 2016-09-16 [PMID: 27631609] (IHC, Mouse)
• Hansen A, Henderson S, Lagos D et al. KSHV-encoded miRNAs target MAF to induce endothelial cell reprogramming. Genes Dev. 2010-01-15 [PMID: 20080955] (WB, Human)
  
  Details:
  WB (Primary human lymphatic epithelial cells): Figs 3C, 4C, 6B.

Reviews for c-Maf Antibody (NBP2-24551) (3)

Average Rating: 3.3

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Verified Customer WB Human 09/13/2021

Summary

• Application: Western Blot
• Sample Tested: Breast cancer cells
• Species: Human
• Lot: C-1

reviewed by: Sarah Franklin IHC-P Human 11/11/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: M1/M2 cell lines
• Species: Human
• Lot: 11082976A

reviewed by: Verified Customer ICC Human 07/28/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: Multiple myeloma cells lines MY5 & RPMI-8226 and T cell leukemia cell line Jurkat cytospins
• Species: Human
• Lot: 11082976A

Bioinformatics

• Gene Symbol: MAF
• Uniprot:
  • O75444()