Western Blot: c-Maf Antibody [NBP2-24551] - Total protein from Human Pancreas and Human Small Intestine was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more
Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human liver cancer using c-MAF antibody at 2ug/ml concentration (1:500 dilution). ...read more
Western Blot: c-Maf Antibody [NBP2-24551] - Analysis of Lysate from human brain in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain probed at 3 ug/ml.
Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - Staining of Human kidney probed with c-maf antibody at 10 ug/ml.
Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - Staining of Human placenta probed with c-maf antibody at 10 ug/ml.
Zeiss AxioImager, FITC filter cube for 400 ms exposure
Immersion-fixed in 4% paraformaldehyde 10 minutes, 0.5% Triton X-100 5 minutes permeabilization at room temperature
PBS 3X quick washes after each step. PBS 3X 10 minutes before mounting in DAPI/Vectashield.
MY5 & RPMI-8226 should have nuclear staining (see Novus #MAB8226) and Jurkat should be negative.Novus notes: This customer tested the product in an untested application that was not listed as a guaranteed application on the datasheet. For this they earn a voucher for 100% of the purchase price as part of our Innovator's Reward Program.
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