MafB Antibody (OTI1E9)

Western Blot: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAFB.

Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human lung tissue.

Flow Cytometry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by MAFB antibody.

Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human breast tissue.

Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human pancreas tissue.

Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human ovary tissue.

Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human Ovary tissue.

Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human endometrium tissue.

Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human pancreas tissue.

Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human bladder tissue.

Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human lymphoma tissue.

Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human liver tissue.

Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human breast tissue.

Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human tonsil tissue.

Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human Kidney tissue.

Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human thyroid tissue.

Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human colon tissue.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: OTI1E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

MafB Antibody (OTI1E9) Summary

• Description: Novus Biologicals Mouse MafB Antibody (OTI1E9) (NBP2-45718) is a monoclonal antibody validated for use in IHC, WB and Flow. Anti-MafB Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 32-320 of human MAFB (NP_005452) produced in E.coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAFB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Application Notes: Heat-induced epitope retrieval by 10mM citric buffer, pH 6.0, 100C for 10 min is recommended
• Theoretical MW: 35.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MafB Antibody (OTI1E9)

• Kreisler (mouse) maf-related leucine zipper homolog
• Kreisler maf-related leucine zipper homolog
• KRML
• KRMLMAFB/Kreisler basic region/leucine zipper transcription factor
• MafB
• Maf-B
• MGC43127
• transcription factor MafB
• v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian)
• V-maf musculoaponeurotic fibrosarcoma oncogene homolog B

Background

MAFB is encoded by this gene is a basic leucine zipper (bZIP) transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The encoded nuclear protein represses ETS1-mediated transcription of erythroid-specific genes in myeloid cells. This gene contains no introns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MafB Antibody (NBP2-45718)(1)

Publication using NBP2-45718

• Hoenow S, Yan K, Noll J Et al. The Properties of Proinflammatory Ly6C(hi) Monocytes Are Differentially Shaped by Parasitic and Bacterial Liver Infections Cells 2022-08-26 [PMID: 36010615] (FLOW, Mouse)
  
  Details:
  Citation using the PerCP version of this antibody.

Bioinformatics

• Gene Symbol: MAFB