BRIP1/FANCJ Antibody (pp15-IB4) - BSA Free

Western Blot: BRIP1/FANCJ Antibody (pp15-IB4) [NB100-189] - Used to detect BRIP1/FANCJ in 293 cell lysate.

Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody (pp15-IB4) [NB100-189] - Analysis of a FFPE tissue section of the human breast cancer using 1:200 dilution of BRIP1/FANCJ antibody (NB100-189). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated a nuclear stain in ductal cells with some cytoplasmic localization.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clone: pp15-IB4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

BRIP1/FANCJ Antibody (pp15-IB4) - BSA Free Summary

• Immunogen: A peptide corresponding to the C-terminus of human FANCJ (within amino acids 1200-1249). [SwissProt# Q9BX63]
• Localization: Nuclear
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BRIP1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Nuclear Cell Lysate
  HeLa Whole Cell Lysate
  Raji Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for BRIP1/FANCJ Antibody (pp15-IB4) - BSA Free

• ATP-dependent RNA helicase BRIP1
• BACH1
• BACH1BRCA1/BRCA2-associated helicase 1
• BRCA1 interacting protein C-terminal helicase 1
• BRCA1-associated C-terminal helicase 1
• BRCA1-binding helicase-like protein BACH1
• BRCA1-interacting protein 1
• BRCA1-interacting protein C-terminal helicase 1
• BRIP1
• EC 3.6.1
• EC 3.6.4.13
• FACJ
• FANCJ
• FANCJFanconi anemia group J protein
• FLJ90232
• MGC126521
• MGC126523
• OF
• Protein FACJ

Background

FANCJ (Fanconi anemia group J protein) was originally identified as a protein which binds BRCT domains of BRCA1 and was named BACH1 (BRCA1-Associated C-terminal Helicase1) or BRIP1 (BRCA1 Interacting Protein C-terminal Helicase1), and after its identification as a component of Fanconi anemia pathway, it was renamed as FANCJ. It is expressed ubiquitously with highest levels in testes and sub-cellularly, it localizes to the nucleus. FANCJ is a DNA-dependent ATPase with 5' to 3' DNA helicase activity which is essential for genetic and structural integrity of genome. FANCJ acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination, and is implicated in repairing DNA double-strand breaks through homologous recombination in FANCJ-BRCA1 interactions dependent manner. Cell-cycle regulated phosphorylation of FANCJ is essential for BRCA1 interaction and 4Fe-4S iron-sulfur-binding is required for its helicase activity. The interaction of FANCJ with proteins including MutLalpha complex and BRCA1, suggests its importance in other pathways of DNA repair and in diseases such as colon and breast cancer. Defective FANCJ causes chromosome instability disorder Fanconi anemia complementation group J.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BRIP1/FANCJ Antibody (NB100-189)(4)

Publications using NB100-189

• Xi H, Gao YH, Han DY et al. Hypoxia inducible factor-1a suppresses Peroxiredoxin 3 expression to promote proliferation of CCRCC cells. FEBS Lett. 2014-08-02 [PMID: 25093297]
• Kim JM, Kee Y, Gurtan A et al. Cell cycle-dependent chromatin loading of the Fanconi anemia core complex by FANCM/FAAP24. Blood 2008-05-01 [PMID: 18174376]
• Cannavo, E et al. Characterization of the interactome of the human MutL homologues MLH1, PMS1, and PMS2. J Biol Chem;282(5):2976-86. 2007-02-02 [PMID: 17148452] (WB, Human)
• Cantor, S et al. BACH1, a novel helicase-like protein, interacts directly with BRCA1 and contributes to its DNA repair function. Cell;105(1):149-60. 2001-04-06 [PMID: 11301010] (IP, Human)

Bioinformatics

• Gene Symbol: BRIP1
• Entrez:
  • 83990(Human)
• Uniprot:
  • Q9BX63()