| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-189SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6496-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14361-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26474-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22189-50ul | |||
![Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/BRIP1-FANCJ-Antibody-Western-Blot-NBP1-31883-img0017.jpg "Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunocytochemistry/Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - 293T cells were fixed in 2% paraformaldehyde/culture medium at 37 degrees C for 30 min. Green: BRIP1 protein stained by FANCJ antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/BRIP1-FANCJ-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31883-img0013.jpg)
![Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP1-31883] - SAS xenograft. FANCJ antibody [N1N2], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/BRIP1-FANCJ-Antibody-Immunohistochemistry-Paraffin-NBP1-31883-img0014.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - Expression of BRIP1/FANCJ at the mRNA (graphs) and protein (photos) levels in iPSCs. Gene expression was assessed with RNA-seq in FACS-sorted bone marrow HSCs or with real-time RT-PCR in cultured iPSCs. The box-and-whiskers graphs show the median, 25th and 75th percentile (box) minimum, and maximum values (whiskers) of the FPKM (fragments per kilobase million) or relative expression in comparison to house-keeping genes (geometric mean for HPRT, B2M, and beta-actin), n = 4, * p < 0.05, ** p < 0.01, Mann–Whitney test (HSCs) or Wilcoxon test (iPSCs). Nuclei are labeled with DAPI. Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33445471/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-31883_rabbit-polyclonal-brip1-fancj-antibody-2552023153951.jpg)
![Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - In situ proximity ligation assay (PLA) in HEK293T cells. (A) G4-BRIP1 & (B) G4-HMOX1 interactions (red dots) were visualized in the control group & after DNase or RNase digestion. Most of the G4 interactions were localized in the nucleus (arrows), but some were also present in the cytoplasm. Cells that were fixed in methanol & mouse 1H6 antibodies were used. Nuclei were counterstained with DAPI (blue), where the transmission bright-field images (BF) show the cell morphologies. Scale bar: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33445471), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31883_rabbit-polyclonal-brip1-fancj-antibody-310202415304253.jpg)
![Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - Detection of the PLA signal in sorted hematopoietic stem cells. (A) G4–Brip1 & (B) G4–Hmox1 interactions (green dots) were visualized in HSCs & GMP & MPP cells. G4 interactions were visible in the nucleus (arrows) & in the cytoplasm. Nuclei were counterstained with DAPI (blue), where the transmission bright-field images (BF) show the cell morphologies. Cells were fixed in PFA & goat 1H6 antibodies were used. Scale bars: 5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33445471), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31883_rabbit-polyclonal-brip1-fancj-antibody-310202415175248.jpg)
![Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRIP1/FANCJ antibody [N1N2], N-term (NBP1-31883) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31883_rabbit-polyclonal-brip1-fancj-antibody-91020242014665.jpg)
![Western Blot: BRIP1/FANCJ Antibody (pp15-IB4) [NB100-189] - Used to detect BRIP1/FANCJ in 293 cell lysate.](http://images.novusbio.com/fullsize/BRIP1-FANCJ-Antibody-pp15-IB4-Western-Blot-NB100-189-img0004.jpg "Western Blot: BRIP1/FANCJ Antibody (pp15-IB4) [NB100-189] - Used to detect BRIP1/FANCJ in 293 cell lysate.")
![Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody (pp15-IB4) [NB100-189] - Analysis of a FFPE tissue section of the human breast cancer using 1:200 dilution of BRIP1/FANCJ antibody (NB100-189). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated a nuclear stain in ductal cells with some cytoplasmic localization.](http://images.novusbio.com/fullsize/BRIP1-FANCJ-Antibody-pp15-IB4-Immunohistochemistry-Paraffin-NB100-189-img0005.jpg)
![Western Blot: BRIP1/FANCJ Antibody [NBP2-14361] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-BRIP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/BRIP1-FANCJ-Antibody-Western-Blot-NBP2-14361-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: BRIP1/FANCJ Antibody [NBP2-14361] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-BRIP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BRIP1/FANCJ Antibody [NBP2-14361] - Staining of human cell line A-431 shows positivity in nucleus and nuclear membrane.](http://images.novusbio.com/fullsize/BRIP1-FANCJ-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14361-img0003.jpg)
![Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP2-14361] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/BRIP1-FANCJ-Antibody-Immunohistochemistry-Paraffin-NBP2-14361-img0001.jpg)
![Western Blot: BRIP1/FANCJ Overexpression Lysate [NBP2-06081] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FANCJ.](http://images.novusbio.com/fullsize/FANCJ-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06081-img0001.jpg "Western Blot: BRIP1/FANCJ Overexpression Lysate [NBP2-06081] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FANCJ.")
![Western Blot: BRIP1/FANCJ Antibody (27F4) [NBP3-26474] - Positive Western Blot detected in: MCF7 whole cell lysate, HEPG2 whole cell lysate. All lanes: BRIP1/FANCJ Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 105 kDa Observed band size: 105 kDa](http://images.novusbio.com/fullsize/nbp3-26474_rabbit-brip1-fancj-mab-27f4-2822024114096.jpg "Western Blot: BRIP1/FANCJ Antibody (27F4) [NBP3-26474] - Positive Western Blot detected in: MCF7 whole cell lysate, HEPG2 whole cell lysate. All lanes: BRIP1/FANCJ Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 105 kDa Observed band size: 105 kDa")
![Western Blot: BRIP1/FANCJ Antibody (SR1035) [NBP3-22189] - Western blot analysis using NBP3-22189 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22189_rabbit-brip1-fancj-mab-sr1035-57202316494261.jpg "Western Blot: BRIP1/FANCJ Antibody (SR1035) [NBP3-22189] - Western blot analysis using NBP3-22189 in HeLa cell lysate.")