BRIP1/FANCJ Antibody (652747)

Images

 
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human ...read more
BRIP1/FANCJ was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human BRIP1/FANCJ Monoclonal Antibody (Catalog # MAB6496) at 15 µg/mL overnight at 4 °C. Before incubation ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
652747
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

BRIP1/FANCJ Antibody (652747) Summary

Immunogen
E. coli-derived recombinant human BRIP1/FANCJ
Ser2-Arg160
Accession # Q9BX63
Specificity
Detects human BRIP1/FANCJ in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) FANCA, D2, E, F, G, I, L, M, or recombinant mouse BRIP1/FANCJ is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BRIP1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BRIP1/FANCJ Antibody (652747)

  • ATP-dependent RNA helicase BRIP1
  • BACH1
  • BACH1BRCA1/BRCA2-associated helicase 1
  • BRCA1 interacting protein C-terminal helicase 1
  • BRCA1-associated C-terminal helicase 1
  • BRCA1-binding helicase-like protein BACH1
  • BRCA1-interacting protein 1
  • BRCA1-interacting protein C-terminal helicase 1
  • BRIP1
  • EC 3.6.1
  • EC 3.6.4.13
  • FACJ
  • FANCJ
  • FANCJFanconi anemia group J protein
  • FLJ90232
  • MGC126521
  • MGC126523
  • OF
  • Protein FACJ

Background

BRCA1-interacting protein 1 (BRIP1), also known as Fanconi anemia complementation group J protein (FANCJ) and BACH1, is a 130 kDa ubiquitously expressed DNA helicase. BRIP1 binds to the BRCT domain of BRCA1 and regulates BRCA1 dependent DNA repair and cell cycle checkpoint control. It is mutated in patients with Fanconi anemia, a disorder characterized by chromosomal instability and increased risk of developing cancer. Within amino acids 1-160, human BRIP1 shares 78% and 74% aa sequence identity with mouse and rat BRIP1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BRIP1
Uniprot