BRIP1/FANCJ Antibody (652747)

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human BRIP1/FANCJ Monoclonal Antibody (Catalog # MAB6496) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BRIP1/FANCJ at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

BRIP1/FANCJ was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human BRIP1/FANCJ Monoclonal Antibody (Catalog # MAB6496) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hemotoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 652747
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

BRIP1/FANCJ Antibody (652747) Summary

• Immunogen: E. coli-derived recombinant human BRIP1/FANCJ
  Ser2-Arg160
  Accession # Q9BX63
• Specificity: Detects human BRIP1/FANCJ in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) FANCA, D2, E, F, G, I, L, M, or recombinant mouse BRIP1/FANCJ is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BRIP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BRIP1/FANCJ Antibody (652747)

• ATP-dependent RNA helicase BRIP1
• BACH1
• BACH1BRCA1/BRCA2-associated helicase 1
• BRCA1 interacting protein C-terminal helicase 1
• BRCA1-associated C-terminal helicase 1
• BRCA1-binding helicase-like protein BACH1
• BRCA1-interacting protein 1
• BRCA1-interacting protein C-terminal helicase 1
• BRIP1
• EC 3.6.1
• EC 3.6.4.13
• FACJ
• FANCJ
• FANCJFanconi anemia group J protein
• FLJ90232
• MGC126521
• MGC126523
• OF
• Protein FACJ

Background

BRCA1-interacting protein 1 (BRIP1), also known as Fanconi anemia complementation group J protein (FANCJ) and BACH1, is a 130 kDa ubiquitously expressed DNA helicase. BRIP1 binds to the BRCT domain of BRCA1 and regulates BRCA1 dependent DNA repair and cell cycle checkpoint control. It is mutated in patients with Fanconi anemia, a disorder characterized by chromosomal instability and increased risk of developing cancer. Within amino acids 1-160, human BRIP1 shares 78% and 74% aa sequence identity with mouse and rat BRIP1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BRIP1
• Uniprot:
  • Q9BX63()