BNIP3L Antibody (S01-0F5)

Western Blot: BNIP3L Antibody (S01-0F5) [NBP3-19585] - Western blot detection of BNIP3L in Jurkat cell lysates using NBP3-19585 (1:500 diluted). Predicted band size: 24kDa. Observed band size: 38kDa.

Immunohistochemistry-Paraffin: BNIP3L Antibody (S01-0F5) [NBP3-19585] - Immunohistochemistry of BNIP3L in paraffin-embedded Human lung cancer tissue using NBP3-19585 at dilution 1/50

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: S01-0F5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

BNIP3L Antibody (S01-0F5) Summary

• Description: Novus Biologicals Rabbit BNIP3L Antibody (S01-0F5) (NBP3-19585) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human BNIP3L (Uniprot # O60238)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BNIP3L
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/20-1/100
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000-1/5000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for BNIP3L Antibody (S01-0F5)

• Adenovirus E1B19K-binding protein B5
• BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A
• BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
• BCL2/adenovirus E1B 19-kd protein-interacting protein 3a
• BCL2/adenovirus E1B 19kDa interacting protein 3-like
• BNIP3a
• BNIP3H
• BNIP3L
• Nip3L
• NIP3-like protein X
• Nix
• NIXBCL2/adenovirus E1B 19kD-interacting protein 3-like

Background

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growingsubclass of the Bcl-2 family. A novel BH3 domaincontaining protein was recently identified and designated Bnip3L, Bnip3a, and Nix (for Nip3-like protein X) (Matsushima, M. et al.; Yasuda, M. et al.; Chen, G, et al.).Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Over expression of Bnip3L induces apoptosis (Yasuda, M. et al.; Chen, G, et al.). Bnip3L interacts withand overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3Lis localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (Matsushima, M. et al.; Yasuda, M. et al.). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BNIP3L