BNIP3L Antibody (HL1203) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein at mitochondria by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:500. Blue: Fluoroshield with DAPI .

Western Blot: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/Immunofluorescence: BNIP3L Antibody (HL1203) - Azide and BSA Free [NBP3-25384] - BNIP3L antibody [HL1203] detects BNIP3L protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BNIP3L stained by BNIP3L antibody [HL1203] (NBP3-25384) diluted at 1:500. Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu, Rt, Bv, Ch, RM
• Applications: WB, ICC/IF, IHC
• Clone: HL1203
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

BNIP3L Antibody (HL1203) - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human BNIP3L. The exact sequence is proprietary.
• Predicted Species: Bovine (100%), Rhesus Macaque (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BNIP3L
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (80%), Xenopus (86%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for BNIP3L Antibody (HL1203) - Azide and BSA Free

• Adenovirus E1B19K-binding protein B5
• BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A
• BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
• BCL2/adenovirus E1B 19-kd protein-interacting protein 3a
• BCL2/adenovirus E1B 19kDa interacting protein 3-like
• BNIP3a
• BNIP3H
• BNIP3L
• Nip3L
• NIP3-like protein X
• Nix
• NIXBCL2/adenovirus E1B 19kD-interacting protein 3-like

Background

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growingsubclass of the Bcl-2 family. A novel BH3 domaincontaining protein was recently identified and designated Bnip3L, Bnip3a, and Nix (for Nip3-like protein X) (Matsushima, M. et al.; Yasuda, M. et al.; Chen, G, et al.).Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Over expression of Bnip3L induces apoptosis (Yasuda, M. et al.; Chen, G, et al.). Bnip3L interacts withand overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3Lis localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (Matsushima, M. et al.; Yasuda, M. et al.). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BNIP3L