BMP-3 Antibody - BSA Free

Western Blot: BMP-3 Antibody [NBP2-92799] - Analysis of extracts of various cell lines, using BMP-3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.

Immunohistochemistry-Paraffin: BMP-3 Antibody [NBP2-92799] - Paraffin-embedded rat kidney using BMP-3 .

Immunohistochemistry-Paraffin: BMP-3 Antibody [NBP2-92799] - Paraffin-embedded human stomach using BMP-3 .

Immunohistochemistry-Paraffin: BMP-3 Antibody [NBP2-92799] - Paraffin-embedded mouse heart using BMP-3 .

Immunohistochemistry-Paraffin: BMP-3 Antibody [NBP2-92799] - Paraffin-embedded rat heart using BMP-3 .

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BMP-3 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 243-472 of human BMP3 (NP_001192.2). YANDAAISEPESVVSSLQGHRNFPTGTVPKWDSHIRAALSIERRKKRSTGVLLPLQNNELPGAEYQYKKDEVWEERKPYKTLQAQAPEKSKNKKKQRKGPHRKSQTLQFDEQTLKKARRKQWIEPRNCARRYLKVDFADIGWSEWIISPKSFDAYYCSGACQFPMPKSLKPSNHATIQSIVRAVGVVPGIPEPCCVPEKMSSLSILFFDENKNVVLKVYPNMTVESCACR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BMP3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL.
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for BMP-3 Antibody - BSA Free

• BMP3
• BMP-3
• BMP3A
• BMP-3A
• bone morphogenetic protein 3 (osteogenic)
• bone morphogenetic protein 3
• Bone morphogenetic protein 3A
• bone morphogenetic protein-3
• Osteogenin

Background

Bone morphogenetic protein 3 (BMP3) is a 472 amino acid member of the bone morphogenetic protein (BMP) family. BMP3 negatively regulates bone density and is a member of the TGF beta superfamily. It is highly expressed in fracture tissue, predominantly in osteoblasts, osteoclasts and chondroblasts. It may play a role in otosclerosis, osteosarcoma, osteogenesis imperfecta, osteomalacia and periodontitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BMP3