Recombinant Human BMP-5 (CHO-expressed) Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human BMP-5 (CHO-expressed) Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.2‑1.2 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human BMP-5 protein
Ala317-His454
Accession #
N-terminal Sequence
Ala317
Protein/Peptide Type
Recombinant Proteins
Gene
BMP5
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
18-23 kDa, reducing conditions
Publications
Read Publications using
615-BMC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-5 (CHO-expressed) Protein

  • BMP5
  • BMP-5
  • bone morphogenetic protein 5
  • MGC34244

Background

Bone Morphogenetic Protein-5 (BMP-5) is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor  beta (TGF-beta ) superfamily (1). BMP-5 is synthesized as a 454 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 20 kDa C-terminal mature protein (2). Mature BMP-5 contains seven conserved cysteine residues involved in formation of the cysteine knot and the single interchain disulfide bond. Biologically active BMP-5 is a disulfide-linked homodimer of the C-terminal mature protein. Mature human BMP-5 shares 96% aa sequence identity with mouse and rat BMP-5. Cellular responses to BMP-5 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors (1). BMP-5 is expressed by chondrocytes in proliferating and hypertrophic zones of bone growth plates (3). It contributes to limb development by promoting proliferation and differentiation of chondrocytes as well as apoptosis of undifferentiated mesoderm (3, 4). Genetic defects in BMP-5 which cause C-terminal truncation or loss of the proteolytic cleavage site result in multiple skeletal abnormalities, including the short ear phenotype in mice (5, 6). BMP-5 is also expressed by ovarian granulosa cells where it functions as an autocrine factor to promote GC proliferation and inhibit their production of progesterone (7). In the nervous system, BMP-5 promotes dendrite outgrowth and dopaminergic neuronal differentiation (8, 9). It is up-regulated in oral squamous carcinoma cells and induces the apoptosis of some myeloma cell lines (10, 11).
  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843.
  3. Mailhot, G. et al. (2008) J. Cell. Physiol. 214:56.
  4. Zuzarte-Luis, V. et al. (2004) Dev. Biol. 272:39.
  5. King, J.A. et al. (1994) Dev. Biol. 166:112.
  6. Ho, A.M. et al. (2008) BMC Dev. Biol. 8:35.
  7. Pierre, A. et al. (2005) Biol. Reprod. 73:1102.
  8. Beck, H.N. et al. (2001) BMC Neurosci. 2:12.
  9. Brederlau, A. et al. (2002) Mol. Cell. Neurosci. 21:367.
  10. Jin, Y. et al. (2001) Oral Oncol. 37:225.
  11. Ro, T.B. et al. (2004) Oncogene 23:3024.

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Publications for BMP-5 (615-BMC)(6)

We have publications tested in 2 confirmed species: Human, Chicken.

We have publications tested in 3 applications: Bioassay, In Vivo, Surface Plasmon Resonance.


Filter By Application
Bioassay
(3)
In Vivo
(1)
Surface Plasmon Resonance
(2)
All Applications
Filter By Species
Human
(3)
Chicken
(3)
All Species
Showing Publications 1 - 6 of 6.
Publications using 615-BMC Applications Species
J Steinfeld, I Steinfeld, A Bausch, N Coronato, ML Hampel, H Depner, PG Layer, A Vogel-Höpk BMP-induced reprograming of the retina into RPE requires WNT signalling in the developing chick optic cup Biol Open, 2017;0(0):. 2017 [PMID: 28546339] (In Vivo, Chicken) In Vivo Chicken
Mann Z, Thiede B, Chang W, Shin J, May-Simera H, Lovett M, Corwin J, Kelley M A gradient of Bmp7 specifies the tonotopic axis in the developing inner ear. Nat Commun, 2014;5(0):3839. 2014 [PMID: 24845721] (Bioassay, Chicken) Bioassay Chicken
Lorda-Diez C, Montero J, Choe S, Garcia-Porrero J, Hurle J Ligand- and stage-dependent divergent functions of BMP signaling in the differentiation of embryonic skeletogenic progenitors in vitro. J Bone Miner Res, 2014;29(3):735-48. 2014 [PMID: 24038612] (Bioassay, Chicken) Bioassay Chicken
Wu, Qifang, Sun, Chia Chi, Lin, Herbert, Babitt, Jodie L Repulsive guidance molecule (RGM) family proteins exhibit differential binding kinetics for bone morphogenetic proteins (BMPs). PLoS ONE, 2012;7(9):e46307. 2012 [PMID: 23029472] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 2011;286(34):30034-46. 2011 [PMID: 21737454] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human

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Bioinformatics

Gene Symbol BMP5
Uniprot