BMP-2/BMP-4 Antibody (100230) [Unconjugated]

Recombinant Human BMP‑2 (Catalog # 355-BM) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Human BMP‑2 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP-2/BMP-4 Monoclonal Antibody (Catalog # MAB3552). The ND50 is typically 5-15 µg/mL in the presence of heparin (2 µg/mL).

• Reactivity: Hu
• Applications: WB, B/N
• Clone: 100230
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

BMP-2/BMP-4 Antibody (100230) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human BMP-2
  Gln283-Arg396
  Accession # P12643
• Specificity: Detects human BMP-2 and human BMP-4 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant zebra fish (rzf) BMP-2 and rhBMP-4, 20%-40% cross-reactivity with rhBMP-3, -3b, -6, and no cross-reactivity with rhBMP-5, -7, -10, or -15 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BMP2
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Neutralization 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMP-2/BMP-4 Antibody (100230) [Unconjugated]

• BDA2
• BMP-2
• BMP-2/BMP-4
• BMP2A
• BMP-2A
• BMP2ABone morphogenetic protein 2A
• bone morphogenetic protein 2

Background

Bone Morphogenetic Protein 2 (BMP-2) and BMP-4 are two of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor  beta (TGF-beta ) superfamily, BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BMP-2/BMP-4 Antibody (MAB3552)(6)

Publications using MAB3552

• Martinez V, Sacedon R, Hidalgo L, Valencia J, Fernandez-Sevilla L, Hernandez-Lopez C, Vicente A, Varas A The BMP Pathway Participates in Human Naive CD4+ T Cell Activation and Homeostasis. PLoS ONE, 2015;10(6):e0131453. 2015 [PMID: 26110906] (Bioassay, Human)
• Boswell BA, Overbeek PA, Musil LS Essential role of BMPs in FGF-induced secondary lens fiber differentiation. Dev. Biol., 2008;324(2):202-12. 2008 [PMID: 18848538] (Neutralization, Chicken)
• Southwood M, Jeffery TK, Yang X, Upton PD, Hall SM, Atkinson C, Haworth SG, Stewart S, Reynolds PN, Long L, Trembath RC, Morrell NW Regulation of bone morphogenetic protein signalling in human pulmonary vascular development. J. Pathol., 2008;214(1):85-95. 2008 [PMID: 17992660] (IHC, Human)
• Lin L, Valore EV, Nemeth E, Goodnough JB, Gabayan V, Ganz T Iron transferrin regulates hepcidin synthesis in primary hepatocyte culture through hemojuvelin and BMP2/4. Blood, 2007;110(6):2182-9. 2007 [PMID: 17540841] (Neutralization, Mouse)
• Rydziel S, Stadmeyer L, Zanotti S, Durant D, Smerdel-Ramoya A, Canalis E Nephroblastoma overexpressed (Nov) inhibits osteoblastogenesis and causes osteopenia. J. Biol. Chem., 2007;282(27):19762-72. 2007 [PMID: 17500060] (Western Blot, Mouse)
• Zhao M, Zhao Z, Koh JT, Jin T, Franceschi RT Combinatorial gene therapy for bone regeneration: cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7. J. Cell. Biochem., 2005;95(1):1-16. 2005 [PMID: 15759283] (Immunoprecipitation, Mouse)

Bioinformatics

• Gene Symbol: BMP2
• Uniprot:
  • P12643()