beta-Catenin Antibody

Images

 
Western Blot: beta-Catenin Antibody [NBP1-89989] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-89989] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining in human cervix, uterine and skeletal muscle tissues. Corresponding CTNNB1 RNA-seq data are presented for the same tissues.
Western Blot: beta-Catenin Antibody [NBP1-89989] - Analysis in human cell line SW480.
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human cervix, uterine shows moderate membranous positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human duodenum shows moderate membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human pancreas shows moderate membranous positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human prostate shows moderate membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-89989] - Staining of human skeletal muscle shows weak positivity in myocytes.

Product Details

Summary
Reactivity Hu, Mu, Rt, FiSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

beta-Catenin Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAH
Marker
Epithelial Cell Marker, Adherens Junction Marker
Specificity
Specificity of beta-Catenin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CTNNB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Positive Control
beta-Catenin Lysate (NBP2-64913)
Control Peptide
beta-Catenin Protein (NBP1-89989PEP)
Publications
Read Publications using NBP1-89989.

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 25502575).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for beta-Catenin Antibody

  • bCatenin
  • beta 1 (88kD)
  • beta-Catenin
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • catenin beta-1
  • CTNNB
  • CTNNB1
  • DKFZp686D02253
  • FLJ25606
  • FLJ37923

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-89989
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Applications: WB, ICC/IF, IHC, IHC-P

Publications for beta-Catenin Antibody (NBP1-89989)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-Catenin Antibody (NBP1-89989) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for beta-Catenin Antibody (NBP1-89989)

Discover related pathways, diseases and genes to beta-Catenin Antibody (NBP1-89989). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for beta-Catenin Antibody (NBP1-89989)

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Blogs on beta-Catenin.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Notch1 - A multifunctional transmembrane receptor
Notch1 is a member of the Notch family of Type 1 single-pass transmembrane proteins that share an extracellular domain of multiple epidermal growth factor-like (EGF) repeats. Notch family members play key roles in a variety of developmental process...  Read full blog post.

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Bioinformatics

Gene Symbol CTNNB1
Entrez
Uniprot