Recombinant Mouse Bcl-xL Protein

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SDS-Page: Mouse Bcl-xL Protein [NBP2-35141]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Func, PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

Recombinant Mouse Bcl-xL Protein Summary

Description
A single, non-glycosylated biologically active polypeptide chain corresponding to 211 residues of BCL2L1.

Source: E. coli

Amino Acid Sequence: SQSNRELVVD FLSYKLSQKG YSWSQFSDVE ENRTEAPEET EAERETPSAI NGNPSWHLAD SPAVNGATGH SSSLDAREVI PMAAVKQALR EAGDEFELRY RRAFSDLTSQ LHITPGTAYQ SFEQVVNELF RDGVNWGRIV AFFSFGGALC VESVDKEMQV LVSRIASWMA TYLNDHLEPW IQENGGWDTF VDLYGNNAAA ESRKGQERFN R

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
Test in Process.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
BCL2L1
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
23.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4, 5 % Trehalose.
Preservative
5% Trehalose
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Mouse Bcl-xL Protein

  • Apoptosis regulator Bcl-X
  • bcl2-L-1
  • BCL2-like 1
  • bcl-2-like protein 1
  • BCLXBCL2LBcl-X
  • BclxL
  • Bcl-xL
  • BCL-XL/S
  • BCLXS
  • bcl-xS
  • DKFZp781P2092

Background

Bcl-X, also named as BCL2L1 or BCL2L, belongs to the Bcl-2 family and is encoded by the BCL2L1 gene in human. Alternative splicing of Bcl-X results in at least two isoforms, isoform Bcl-X(L) (also named as Bcl-xL) and isoform Bcl-x(S) (also named as Bcl-xS). Bcl-xL is found in tissues containing long-lived postmitotic cells, such as adult brain, while Bcl-xS is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. Bcl-X forms homodimer or heterodimer with other Bcl-2 proteins, like BAK, BAX or Bcl-2, to act as anti- or pro- apoptotic regulators. Bcl-xL appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane, and it also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. In contrast, Bcl-xS is a pro-apoptotic protein that promotes apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol BCL2L1
Uniprot