Bcl-xL Antibody (2052A) [Alexa Fluor® 532]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 2052A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 532

Bcl-xL Antibody (2052A) [Alexa Fluor® 532] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant human Bcl‑x_L
  Met1-Arg212
  Accession # Q07817
• Specificity: Detects human Bcl‑x_L in direct ELISAs and detects human, mouse, and rat Bcl‑x_L in Western blots.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bcl-xL Antibody (2052A) [Alexa Fluor® 532]

• Apoptosis regulator Bcl-X
• bcl2-L-1
• BCL2-like 1
• bcl-2-like protein 1
• BCLXBCL2LBcl-X
• BclxL
• Bcl-xL
• BCL-XL/S
• BCLXS
• bcl-xS
• DKFZp781P2092

Background

Bcl-x_L is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-x_L is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-x_L is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-x_L contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-x_L missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-x_L appears to be mediated through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.