Bcl-xL Antibody (2052A) [FITC]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 2052A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: FITC

Bcl-xL Antibody (2052A) [FITC] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant human Bcl‑x_L
  Met1-Arg212
  Accession # Q07817
• Specificity: Detects human Bcl‑x_L in direct ELISAs and detects human, mouse, and rat Bcl‑x_L in Western blots.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from cell culture supernatant

Alternate Names for Bcl-xL Antibody (2052A) [FITC]

• Apoptosis regulator Bcl-X
• bcl2-L-1
• BCL2-like 1
• bcl-2-like protein 1
• BCLXBCL2LBcl-X
• BclxL
• Bcl-xL
• BCL-XL/S
• BCLXS
• bcl-xS
• DKFZp781P2092

Background

Bcl-x, a member of the Bcl-2 protein family, inhibits cell death, or apoptosis (1). Bcl-x is expressed as two isomeric forms, Bcl-xL and Bcl-xS, and is typically present in the cytosol in association with the mitochondrial membrane. Bcl-xL forms heterodimers with various proteins, including Bax, Bak and Bcl-2 (2). It has been found that eterodimerization with Bax does not seem to be required for anti-apoptotic activity (3). Since Bcl-xL can form an ion channel in synthetic lipid membranes, there is a strong possibility that this property plays a role in heterodimerization-independent cell survival (4). The Bcl-X(S) isoform promotes apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.