Bcl-9 Antibody - BSA Free

Western Blot: Bcl-9 Antibody [NBP3-05612] - Western blot analysis of extracts of various cell lines, using Bcl-9 antibody (NBP3-05612) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP3-05612] - Immunohistochemistry of paraffin-embedded Mouse testis using Bcl-9 antibody (NBP3-05612) at dilution of 1:100 (20x lens).

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP3-05612] - Immunohistochemistry of paraffin-embedded Rat brain using Bcl-9 antibody (NBP3-05612) at dilution of 1:100 (20x lens).

Immunohistochemistry-Paraffin: Bcl-9 Antibody [NBP3-05612] - Immunohistochemistry of paraffin-embedded Human colon carcinoma using Bcl-9 antibody (NBP3-05612) at dilution of 1:100 (20x lens).

Western Blot: Bcl-9 Antibody - BSA Free [Bcl-9] - Western blot analysis of lysates from wild type (WT) and Bcl-9 knockdown (KD) 293T cells using [KD Validated] Bcl-9 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.

Western Blot: Bcl-9 Antibody - BSA Free [Bcl-9] - Western blot analysis of various lysates using [KD Validated] Bcl-9 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Bcl-9 Antibody - BSA Free Summary

• Immunogen: Recombinant protein of mouse Bcl-9
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCL9
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 148 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Bcl-9 Antibody - BSA Free

• B-cell CLL/lymphoma 9 protein
• B-cell CLL/lymphoma 9
• B-cell lymphoma 9 protein
• Bcl9
• Bcl-9
• BCL9L
• LGS
• MGC131591
• Protein legless homolog

Background

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL9