Bcl-2 Antibody (E17)

Images

 
Western Blot: Bcl-2 Antibody (E17) [NB110-55551] - Jurkat cell lysate.
Immunohistochemistry: Bcl-2 Antibody (E17) [NB110-55551] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-BCL-2 Rab Mab
Flow Cytometry: Bcl-2 Antibody (E17) [NB110-55551] - Analysis of permeabilized Jurkat cells.

Product Details

Summary
Product Discontinued
View other related Bcl-2 Primary Antibodies

Order Details


    • Catalog Number
      NB110-55551
    • Availability
      Product Discontinued

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Bcl-2 Antibody (E17) Summary

Immunogen
A synthetic peptide corresponding to residues between BH3 and BH4 of human Bcl-2 was used as immunogen.
Localization
Endoplasmic reticulum, outer mitochondrial membrane and intracellular membrane of the nuclear envelope.
Specificity
The antibody does not cross-react with other Bcl-2 family members
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
BCL2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Flow Cytometry 1:200
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Immunoprecipitation 1:50
Application Notes
This is a high affinity, high titer antibody. Optimal dilutions should be determined by the end user. (Provided dilutions serve only as a suggestive starting point.)
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Jurkat Lysate (NB800-PC2)
MCF-7 Lysate (NB820-59465)
Publications
Read Publications using NB110-55551.

Reactivity Notes

Species cross reactivity tested by Western Blot only.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Culture supernatant in buffered aqueous solution
Preservative
No Preservative
Concentration
0.01 mg/ml
Purity
Protein A or G purified

Alternate Names for Bcl-2 Antibody (E17)

  • apoptosis regulator Bcl-2
  • B-cell CLL/lymphoma 2
  • Bcl2
  • Bcl-2

Background

The Bcl-2 family of proteins regulate apoptosis by controlling mitochondrial permeability and release of cytochrome c. Bcl-2 is an anti-apoptotic protein that resides in the outer mitochondrial wall and inhibits release of cytochrome c (1). Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies (2). It has also been shown that post-translational modification of Bcl-2, phosphorylation at serine 70, may be required to render its full and/or enhanced anti-apoptotic effect (3, 4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Bcl-2 Antibody (NB110-55551)(14)

We have publications tested in 4 applications: ICC/IF, IHC, IP, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IP
(3)
WB
(8)
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB110-55551 Applications Species
May KL, Paton JC, Paton AW et al. Escherichia coli subtilase cytotoxin induces apoptosis regulated by host Bcl-2 family proteins Bax/Bak. Infect Immun 2010 Nov [PMID: 20713620] (WB) WB
Yecies D, Carlson NE, Deng J et al. Acquired resistance to ABT-737 in lymphoma cells that up-regulate MCL-1 and BFL-1. Blood 2010 Apr [PMID: 20197552] (WB) WB
Idikio HA. Immunohistochemistry in diagnostic surgical pathology: contributions of protein life-cycle, use of evidence-based methods and data normalization on interpretation of immunohistochemical stains. Int J Clin Exp Pathol. 2009 Nov. [PMID: 20126585]
Priault M, Hue E, Marhuenda F et al. Differential dependence on Beclin 1 for the regulation of pro-survival autophagy by Bcl-2 and Bcl-xL in HCT116 colorectal cancer cells. PLoS One 2010 Jan [PMID: 20090905] (IP) IP
Steinacker P, Hawlik A, Lehnert S et al. Neuroprotective function of cellular prion protein in a mouse model of amyotrophic lateral sclerosis. Am J Pathol 2010 Mar [PMID: 20075202] (WB) WB
Winkler J, Martin-Killias P, Pluckthun A et al. EpCAM-targeted delivery of nanocomplexed siRNA to tumor cells with designed ankyrin repeat proteins. Mol Cancer Ther 2009 Sep [PMID: 19723880] (WB) WB
Savage KJ, Johnson NA, Ben-Neriah S et al. MYC gene rearrangements are associated with a poor prognosis in diffuse large B-cell lymphoma patients treated with R-CHOP chemotherapy. Blood 2009 Oct [PMID: 19704118] (WB) WB
Johnson NA, Savage KJ, Ludkovski O et al. Lymphomas with concurrent BCL2 and MYC translocations: the critical factors associated with survival. Blood 2009 Sep [PMID: 19597184] (IHC) IHC
Bai Y, Meng Z, Cui M et al. An Ang1-Tie2-PI3K axis in neural progenitor cells initiates survival responses against oxygen and glucose deprivation. Neuroscience 2009 May [PMID: 19409199] (WB) WB
Eliseev RA, Malecki J, Lester T et al. Cyclophilin D interacts with Bcl2 and exerts an anti-apoptotic effect. J Biol Chem 2009 Apr [PMID: 19228691] (IP) IP
Show All 14 Publications.

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Product General Protocols

View specific protocols for Bcl-2 Antibody (NB110-55551): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Bcl-2 Antibody (NB110-55551) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Bcl-2 Antibody (NB110-55551)

Discover related pathways, diseases and genes to Bcl-2 Antibody (NB110-55551). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Bcl-2 Antibody (NB110-55551)

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Blogs on Bcl-2.

Key Targets in Apoptosis, Necroptosis, and Autophagy
Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and...  Read full blog post.

Altered expression of BCL2 in cancer
Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease.  For example, too little cell death can promote cell overgrowth a...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.

Beclin 1 - a key regulator of autophagosome formation
The Beclin 1 protein is a central regulator of autophagy in mammalian cells. Autophagy is an essential process used to maintain cellular homeostasis by degrading and recycling cellular components such as damaged or worn out organelles and macromole...  Read full blog post.

BNIP3 - a regulator of mitochondrial autophagy and cell death
Bcl-2 nineteen-kilodalton interacting protein 3 (BNIP3) is a pro-apoptotic BH3-only protein. BNIP3 localizes to the mitochondrial membrane where it plays a key role in mitochondrial autophagy and cell death pathways. Similar to other Bcl-2 family m...  Read full blog post.

BAG3 - Hsp70 is my friend!
The BAG proteins are a large family of chaperone regulators governing a wide range of cell processes such as proliferation, survival, stress response, tumorigenesis, neuronal differentiation, growth arrest and apoptosis as reviewed in Takayama et al1....  Read full blog post.

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Bioinformatics

Gene Symbol BCL2
Entrez
Uniprot