BCKDHA Antibody - BSA Free

Western Blot: BCKDHA Antibody [NBP2-97503] - Anti-BCKDHA rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: HeLa Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa

Immunocytochemistry/Immunofluorescence: BCKDHA Antibody [NBP2-97503] - Immunofluorescence staining of BCKDHA in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human BCKDHA polyclonal antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Immunohistochemistry-Paraffin: BCKDHA Antibody [NBP2-97503] - Immunochemical staining of human BCKDHA in human colon carcinoma with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: BCKDHA Antibody [NBP2-97503] - Immunochemical staining of human BCKDHA in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BCKDHA Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human BCKDHA fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCKDHA
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for BCKDHA Antibody - BSA Free

• 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial
• BCKDE1A
• BCKDH E1-alpha
• branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urinedisease)
• branched chain keto acid dehydrogenase E1, alpha polypeptide
• Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain
• EC 1.2.4.4,2-oxoisovalerate dehydrogenase (lipoamide)
• FLJ45695
• MSU
• MSUD1
• OVD1A

Background

BCKDHA - branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urine disease)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCKDHA