BCKDHA Antibodies
We offer BCKDHA Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each BCKDHA Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our BCKDHA Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the BCKDHA Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our BCKDHA polyclonal antibodies.
Alternate Names for BCKDHA Antibodies
anti-BCKDHA antibody, anti-2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial antibody, anti-BCKDE1A antibody, anti-BCKDH E1-alpha antibody, anti-branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urinedisease) antibody, anti-branched chain keto acid dehydrogenase E1, alpha polypeptide antibody, anti-Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain antibody, anti-EC 1.2.4.4,2-oxoisovalerate dehydrogenase (lipoamide) antibody, anti-FLJ45695 antibody, anti-MSU antibody, anti-MSUD1 antibody, anti-OVD1A antibody
14 Results for "bckdha" in Primary Antibodies
![Western Blot: BCKDHA Antibody [NBP1-79616] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/BCKDHA-Antibody-Western-Blot-NBP1-79616-img0002.jpg "Western Blot: BCKDHA Antibody [NBP1-79616] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.")
![Immunohistochemistry-Paraffin: BCKDHA Antibody [NBP1-79616] - Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue Observed Staining: Cytoplasmic in alveolar type I & II cells](http://images.novusbio.com/fullsize/BCKDHA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-79616-img0006.jpg)
![Western Blot: BCKDHA Antibody [NBP1-79616] - Human Adult Placenta.](http://images.novusbio.com/fullsize/BCKDHA-Antibody-Western-Blot-NBP1-79616-img0003.jpg)
![Western Blot: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] - Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2405] (NBP3-25380) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25380_rabbit-bckdha-mab-hl2405-azide-and-bsa-free-2120249383170.jpg "Western Blot: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] - Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2405] (NBP3-25380) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2405] (NBP3-25380) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25380_rabbit-bckdha-mab-hl2405-azide-and-bsa-free-2120249342737.jpg)
![Immunocytochemistry/Immunofluorescence: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] - BCKDHA antibody [HL2405] detects BCKDHA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BCKDHA stained by BCKDHA antibody [HL2405] (NBP3-25380) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25380_rabbit-bckdha-mab-hl2405-azide-and-bsa-free-2120249371542.jpg)
![Immunohistochemistry-Paraffin: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] - BCKDHA antibody [HL2405] detects BCKDHA protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. BCKDHA stained by BCKDHA antibody [HL2405] (NBP3-25380) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25380_rabbit-bckdha-mab-hl2405-azide-and-bsa-free-2120249371562.jpg)
![Western Blot: BCKDHA Antibody (HL2434) - Azide and BSA Free [NBP3-25382] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2434] (NBP3-25382) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25382_rabbit-bckdha-mab-hl2434-azide-and-bsa-free-2120249425481.jpg "Western Blot: BCKDHA Antibody (HL2434) - Azide and BSA Free [NBP3-25382] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2434] (NBP3-25382) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: BCKDHA Antibody (HL2434) - Azide and BSA Free [NBP3-25382] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2434] (NBP3-25382) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25382_rabbit-bckdha-mab-hl2434-azide-and-bsa-free-2120249393590.jpg)
![Western Blot: BCKDHA Antibody (HL2434) - Azide and BSA Free [NBP3-25382] - Whole zebrafish extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2434] (NBP3-25382) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25382_rabbit-bckdha-mab-hl2434-azide-and-bsa-free-212024940852.jpg)
![Immunohistochemistry-Paraffin: BCKDHA Antibody (HL2434) - Azide and BSA Free [NBP3-25382] - BCKDHA antibody [HL2434] detects BCKDHA protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. BCKDHA stained by BCKDHA antibody [HL2434] (NBP3-25382) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25382_rabbit-bckdha-mab-hl2434-azide-and-bsa-free-212024935552.jpg)
![Western Blot: BCKDHA Antibody [NBP2-15552] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/BCKDHA-Antibody-Western-Blot-NBP2-15552-img0001.jpg "Western Blot: BCKDHA Antibody [NBP2-15552] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: BCKDHA Antibody [NBP2-15552] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: BCKDHA protein stained by BCKDHA antibody [C1C3] diluted at 1:400. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/BCKDHA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15552-img0004.jpg)
![Immunohistochemistry-Paraffin: BCKDHA Antibody [NBP2-15552] - Human colon carcinoma, using BCKDHA antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/BCKDHA-Antibody-Immunohistochemistry-Paraffin-NBP2-15552-img0002.jpg)
![Immunoprecipitation: BCKDHA Antibody [NBP3-14681] - Whole cell lysate (1 mg per IP reaction, 20% of IP loaded) from Hep-G2 cells treated with phosphatase (+) or mock treated (-).Rabbit anti-BCKDHA antibody used for IP at 6 ug per reaction. For blotting immunoprecipitated rabbit anti-Phospho BCKDHA recombinant monoclonal antibody was used at 1:1000 (upper panel) was used at 1 ug/ml (lower panel). HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BCKDHA-Antibody-Immunoprecipitation-NBP3-14681-img0001.jpg "Immunoprecipitation: BCKDHA Antibody [NBP3-14681] - Whole cell lysate (1 mg per IP reaction, 20% of IP loaded) from Hep-G2 cells treated with phosphatase (+) or mock treated (-).Rabbit anti-BCKDHA antibody used for IP at 6 ug per reaction. For blotting immunoprecipitated rabbit anti-Phospho BCKDHA recombinant monoclonal antibody was used at 1:1000 (upper panel) was used at 1 ug/ml (lower panel). HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 30 seconds.")
![Simple Western: BCKDHA Antibody [NBP3-14681] - Detection of mouse pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody [BLR060F] (NBP3-14681) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp3-14681_rabbit-bckdha-mab-simple-western-3032023111445.jpg)
![Simple Western: BCKDHA Antibody [NBP3-14681] - Detection of human pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody [BLR060F] (NBP3-14681) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp3-14681_rabbit-bckdha-mab-simple-western-3032023111648.jpg)
![Western Blot: BCKDHA Antibody - BSA Free [NBP2-92584] - Western blot analysis of various lysates using BCKDHA Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp2-92584_rabbit-polyclonal-bckdha-antibody-42202514251836.jpg "Western Blot: BCKDHA Antibody - BSA Free [NBP2-92584] - Western blot analysis of various lysates using BCKDHA Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Western Blot: BCKDHA Antibody - BSA Free [NBP2-92584] - Western blot analysis of various lysates, using BCKDHA Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-92584_rabbit-polyclonal-bckdha-antibody-42202514264466.jpg)
![Western Blot: BCKDHA Antibody - BSA Free [NBP2-92584] - Western blot analysis of various lysates, using BCKDHA Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 150s.](http://images.novusbio.com/fullsize/nbp2-92584_rabbit-polyclonal-bckdha-antibody-4220251430498.jpg)
![Western Blot: BCKDHA Antibody [NBP2-97503] - Anti-BCKDHA rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: HeLa Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa](http://images.novusbio.com/fullsize/BCKDHA-Antibody-Western-Blot-NBP2-97503-img0005.jpg "Western Blot: BCKDHA Antibody [NBP2-97503] - Anti-BCKDHA rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: HeLa Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa")
![Immunocytochemistry/Immunofluorescence: BCKDHA Antibody [NBP2-97503] - Immunofluorescence staining of BCKDHA in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human BCKDHA polyclonal antibody (dilution ratio 1:500) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/BCKDHA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97503-img0001.jpg)
![Immunohistochemistry-Paraffin: BCKDHA Antibody [NBP2-97503] - Immunochemical staining of human BCKDHA in human colon carcinoma with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/BCKDHA-Antibody-Immunohistochemistry-Paraffin-NBP2-97503-img0004.jpg)
![Immunohistochemistry-Paraffin: BCKDHA Antibody [NBP2-97503] - Immunochemical staining of human BCKDHA in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/BCKDHA-Antibody-Immunohistochemistry-Paraffin-NBP2-97503-img0003.jpg)
![Western Blot:[NBP3-30366]-Detection of human pBCKDHA by western blot. Samples: Whole cell lysate from Hep-G2, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antibody used for WB at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30366_rabbit-bckdha-pab-231202511585516.jpg "Western Blot:[NBP3-30366]-Detection of human pBCKDHA by western blot. Samples: Whole cell lysate from Hep-G2, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antibody used for WB at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation:BCKDHA AntibodyNBP3-29784] -Detection of human BCKDHA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BCKDHA antibody NBP3-29784 (lot NBP3-29784-2) used for IP at 6 µg per reaction. BCKDHA was also immunoprecipitated by a previous lot of this antibody (lot NBP3-29784-1) and rabbit anti-BCKDHA.. For blotting immunoprecipitated BCKDHA, NBP3-29784 was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29784_rabbit-bckdha-pab-3012202416502210.jpg "Immunoprecipitation:BCKDHA AntibodyNBP3-29784] -Detection of human BCKDHA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BCKDHA antibody NBP3-29784 (lot NBP3-29784-2) used for IP at 6 µg per reaction. BCKDHA was also immunoprecipitated by a previous lot of this antibody (lot NBP3-29784-1) and rabbit anti-BCKDHA.. For blotting immunoprecipitated BCKDHA, NBP3-29784 was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: BCKDHA Antibody [NBP1-81022] - Staining of human kidney shows strong granular positivity in cells in tubules.](http://images.novusbio.com/fullsize/BCKDHA-Antibody-Immunohistochemistry-Paraffin-NBP1-81022-img0004.jpg "Immunohistochemistry-Paraffin: BCKDHA Antibody [NBP1-81022] - Staining of human kidney shows strong granular positivity in cells in tubules.")
![Western Blot: BCKDHA Antibody [H00000593-B01P] - Analysis of BCKDHA expression in transfected 293T cell line by BCKDHA polyclonal antibody. Lane 1: BCKDHA transfected lysate(48.95 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BCKDHA-Antibody-Western-Blot-H00000593-B01P-img0002.jpg "Western Blot: BCKDHA Antibody [H00000593-B01P] - Analysis of BCKDHA expression in transfected 293T cell line by BCKDHA polyclonal antibody. Lane 1: BCKDHA transfected lysate(48.95 KDa). Lane 2: Non-transfected lysate.")
![Immunoprecipitation:BCKDHA AntibodyNBP3-29423] -Detection of human BCKDHA by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BCKDHA antibody NBP3-29423 (lot NBP3-29423-1) used for IP at 6 µg/mg lysate. BCKDHA was also immunoprecipitated by rabbit anti-BCKDHA., which recognizes a downstream epitope. For blotting immunoprecipitated BCKDHA, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29423_rabbit-bckdha-pab-30122024152143.jpg "Immunoprecipitation:BCKDHA AntibodyNBP3-29423] -Detection of human BCKDHA by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BCKDHA antibody NBP3-29423 (lot NBP3-29423-1) used for IP at 6 µg/mg lysate. BCKDHA was also immunoprecipitated by rabbit anti-BCKDHA., which recognizes a downstream epitope. For blotting immunoprecipitated BCKDHA, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")