BCKDHA Antibody (BLR060F)

Immunoprecipitation: BCKDHA Antibody [NBP3-14681] - Whole cell lysate (1 mg per IP reaction, 20% of IP loaded) from Hep-G2 cells treated with phosphatase (+) or mock treated (-).Rabbit anti-BCKDHA antibody used for IP at 6 ug per reaction. For blotting immunoprecipitated rabbit anti-Phospho BCKDHA recombinant monoclonal antibody was used at 1:1000 (upper panel) was used at 1 ug/ml (lower panel). HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 30 seconds.

Simple Western: BCKDHA Antibody [NBP3-14681] - Detection of mouse pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody [BLR060F] (NBP3-14681) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.

Simple Western: BCKDHA Antibody [NBP3-14681] - Detection of human pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody [BLR060F] (NBP3-14681) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, IP
• Clone: BLR060F
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

BCKDHA Antibody (BLR060F) Summary

• Description: Novus Biologicals Rabbit BCKDHA Antibody (BLR060F) (NBP3-14681) is a recombinant monoclonal antibody validated for use in WB, Simple Western and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal
• Immunogen: surrounding serine 292
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BCKDHA
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Simple Western 1:10 - 1:50
  • Western Blot
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Tested in 3T3 [0.4 mg/mL], separated by Size, antibody dilution of 1:10, 1:50, apparent MW was 52 kDa

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
• Preservative: 0.09% Sodium Azide
• Purity: >95%

Alternate Names for BCKDHA Antibody (BLR060F)

• 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial
• BCKDE1A
• BCKDH E1-alpha
• branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urinedisease)
• branched chain keto acid dehydrogenase E1, alpha polypeptide
• Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain
• EC 1.2.4.4,2-oxoisovalerate dehydrogenase (lipoamide)
• FLJ45695
• MSU
• MSUD1
• OVD1A

Background

BCKDHA - branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urine disease)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCKDHA