B7-1/CD80 Antibody (62N3G8)

Images

 
Western Blot: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Analysis in human Raji lymphoma cells.
Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Human thymus stained with CD80 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen. Note membrane and cytoplasmic staining.
Flow Cytometry: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Raji lymphoma cells. Image from verified customer review.
Western Blot: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Analysis of CD80 in A) a partial recombinant protein and B) Daudi lysate using this antibody at 0.1 and 1 g/ml, respectively. Goat anti-mouse Ig HRP secondary ...read more
Flow Cytometry: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25255. Staining of CD80 in human PBMC (monocyte depleted) using anti-CD80 antibody. Image from verified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
62N3G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

B7-1/CD80 Antibody (62N3G8) Summary

Immunogen
Amino acids 102-223 of human CD80 were used as the immunogen for the antibody.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD80
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Flow and WB data from customer review.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP2-25255 in the following applications:

Publications
Read Publication using NBP2-25255.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for B7-1/CD80 Antibody (62N3G8)

  • Activation B7-1 antigen
  • B7
  • B71
  • B7-1
  • BB1
  • CD28LG1
  • CD28LGB7-1 antigen)
  • CD80 antigen
  • CD80 molecule
  • CD80
  • costimulatory factor CD80
  • costimulatory molecule variant IgV-CD80
  • CTLA-4 counter-receptor B7.1
  • T-lymphocyte activation antigen CD80

Background

The CD80 antigen (B7-1, BB1) recognizes a 60-kD transmembrane glycoprotein that is a member of the immunoglobulin family. This molecule shares with CD86 the capability to be the ligand for two structurally similar molecules expressed on T lymphocytes, CD28 and CD152 (CTLA-4). CD80 antigen is expressed on in vitro activated B lymphocytes after 24h stimulation and at maximal level 48-72h post activation. It is not expressed on the majority of resting B cells from peripheral blood but identifies a subpopulation of B cells that has been previously activated. The antigen is also expressed by HTLV-1 transformed T cells activated monocytes, and constitutively on dendritic cells. CD80 binding provides costimulatory signals for T cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for B7-1/CD80 Antibody (NBP2-25255)(1)

Reviews for B7-1/CD80 Antibody (NBP2-25255) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP2-25255:
Filter by Applications
WB
(1)
Flow
(1)
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Western Blot B7-1/CD80 NBP2-25255
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reviewed by:
Anonymous
WB Human 06/28/2018
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Summary

ApplicationWestern Blot
Sample TestedRaji whole cell lysate
SpeciesHuman
LotAB021845613G801
Flow Cytometry B7-1/CD80 NBP2-25255
Enlarge
reviewed by:
Anonymous
Flow Human 06/28/2018
View

Summary

ApplicationFlow Cytometry
Sample TestedRaji lymphoma cells
SpeciesHuman
LotAB021845613G801

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FAQs for B7-1/CD80 Antibody (NBP2-25255) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-25255AF405
Alexa Fluor 488 Labeled NBP2-25255AF488
Alexa Fluor 647 Labeled NBP2-25255AF647
Alexa Fluor 700 Labeled NBP2-25255AF700
Allophycocyanin Labeled NBP2-25255APC
Biotin Labeled NBP2-25255B
DyLight 350 Labeled NBP2-25255UV
DyLight 405 Labeled NBP2-25255V
DyLight 488 Labeled NBP2-25255G
DyLight 550 Labeled NBP2-25255R
DyLight 650 Labeled NBP2-25255C
DyLight 680 Labeled NBP2-25255FR
DyLight 755 Labeled NBP2-25255IR
HRP Labeled NBP2-25255H
PE Labeled NBP2-25255PE

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Bioinformatics Tool for B7-1/CD80 Antibody (NBP2-25255)

Discover related pathways, diseases and genes to B7-1/CD80 Antibody (NBP2-25255). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for B7-1/CD80 Antibody (NBP2-25255)

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Pathways for B7-1/CD80 Antibody (NBP2-25255)

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PTMs for B7-1/CD80 Antibody (NBP2-25255)

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Research Areas for B7-1/CD80 Antibody (NBP2-25255)

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Blogs on B7-1/CD80.

CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
06/28/2018
Application: WB
Species: Human

Anonymous
06/28/2018
Application: Flow
Species: Human

Bioinformatics

Gene Symbol CD80
Uniprot