B7-1/CD80 Research Products: Novus Biologicals

Antibodies

B7-1/CD80 Antibody (37711) [U ...

MAB140

MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox" target="_blank"> Flow Cytometry B7-1/CD80 Antibody (37711) [Unconjugated]

Flow Cytometry B7-1/CD80 Antibody (37711) [Unconjugated]

140-B1) co-stimulates IL-2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL-2 Quantikine ELISA Kit (Catalog # D2050). IL-2 secretion elicited by Recombinant Human B7-1/CD80 Fc Chimera (1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog # MAB140). The ND₅₀ is typically 0.2-1 µg/mL." class="big_lightbox" target="_blank"> Neutralization B7-1/CD80 Antibody (37711) [Unconjugated]

Neutralization B7-1/CD80 Antibody (37711) [Unconjugated]

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank"> Immunohistochemistry B7-1/CD80 Antibody (37711) [Unconjugated]

Immunohistochemistry B7-1/CD80 Antibody (37711) [Unconjugated]

VC001) and DAB chromogen (lower image, brown). Tissues were counterstained with hematoxylin (blue)." class="big_lightbox" target="_blank"> Dual RNAscope ISH-IHC Compatible B7-1/CD80 Antibody (37711) [Unconjugated]

Dual RNAscope ISH-IHC Compatible B7-1/CD80 Antibody (37711) [Unconjugated]

140-B1) was serially diluted and captured by Mouse Anti-Human B7‑1/CD80 Monoclonal Antibody (Catalog # MAB140) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human B7‑1/CD80 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox" target="_blank"> ELISA B7-1/CD80 Antibody (37711) [Unconjugated]

ELISA B7-1/CD80 Antibody (37711) [Unconjugated]

(28 Publications)

Species: Hu
Applications: Flow, IHC, B/N, CyTOF-ready, Dual ISH-IHC, ELISA(Cap)
Host: Mouse Monoclonal

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB140U-100UG
Alexa Fluor 405	FAB140V-100UG
Alexa Fluor 488	FAB140G-100UG
Alexa Fluor 532	FAB140X
Alexa Fluor 594	FAB140T-100UG
Alexa Fluor 647	FAB140R-100UG
Alexa Fluor 700	FAB140N-100UG
Alexa Fluor 750	FAB140S-100UG
Alexa Fluor Plus 405	FAB140AFP405-100UG
Alexa Fluor Plus 488	FAB140AFP488-100UG
Alexa Fluor Plus 555	FAB140AFP555-100UG
Alexa Fluor Plus 594	FAB140AFP594-100UG
Alexa Fluor Plus 647	FAB140AFP647-100UG
Alexa Fluor Plus 680	FAB140AFP680-100UG
Allophycocyanin	FAB140A
Allophycocyanin/Cy7	FAB140APCCY7
Biotin	FAB140B
CoraFluor 1	FAB140CL1
DyLight 350	FAB140D
DyLight 405	FAB140E
DyLight 488	FAB140K
DyLight 550	FAB140L
DyLight 594	FAB140M
DyLight 650	FAB140W
DyLight 680	FAB140Y
DyLight 755	FAB140Z
Fluorescein	FAB140F-025
Fluorescein	FAB140F-100
HRP	FAB140H
Janelia Fluor 525	FAB140JF525
Janelia Fluor 549	FAB140I
Janelia Fluor 646	FAB140J
Janelia Fluor 669	FAB140JF669
PE/Atto594	FAB140PEATT594
PE/Cy5.5	FAB140PECY55
PE/Cy7	FAB140PECY7
PerCP	FAB140C
Phycoerythrin	FAB140P-025
Phycoerythrin	FAB140P-100
mFluor Violet 450 SE	FAB140MFV450
mFluor Violet 500 SE	FAB140MFV500
mFluor Violet 610 SE	FAB140MFV610

Formulation	Catalog #	Availability	Price
Carrier Free	MAB140-SP
Carrier Free	FAB140P-100
Carrier Free	FAB140P-025
Carrier Free	FAB140F-100
Carrier Free	FAB140F-025
Carrier Free	MAB140-100
Carrier Free	MAB140-500
Carrier Free	FAB140V-100UG
Carrier Free	FAB140U-100UG
Carrier Free	FAB140T-100UG
Carrier Free	FAB140S-100UG
Carrier Free	FAB140R-100UG
Carrier Free	FAB140N-100UG
Carrier Free	FAB140G-100UG

B7-1/CD80 Antibody (62N3G8) - ...

NBP2-25255

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox" target="_blank"> Multiplex Immunofluorescence B7-1/CD80 Antibody (62N3G8) - BSA Free

Multiplex Immunofluorescence B7-1/CD80 Antibody (62N3G8) - BSA Free

Western Blot B7-1/CD80 Antibody (62N3G8) - BSA Free

Immunohistochemistry-Paraffin B7-1/CD80 Antibody (62N3G8) - BSA Free

Flow Cytometry B7-1/CD80 Antibody (62N3G8) - BSA Free

(2 Reviews)

(4 Publications)

Species: Hu, Mu
Applications: WB, Flow, IHC, COMET, mIF
Host: Mouse Monoclonal

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25255AF350
Alexa Fluor 405	NBP2-25255AF405
Alexa Fluor 488	NBP2-25255AF488
Alexa Fluor 532	NBP2-25255AF532
Alexa Fluor 594	NBP2-25255AF594
Alexa Fluor 647	NBP2-25255AF647
Alexa Fluor 700	NBP2-25255AF700
Alexa Fluor 750	NBP2-25255AF750
Alexa Fluor Plus 405	NBP2-25255AFP405
Alexa Fluor Plus 488	NBP2-25255AFP488
Alexa Fluor Plus 555	NBP2-25255AFP555
Alexa Fluor Plus 594	NBP2-25255AFP594
Alexa Fluor Plus 647	NBP2-25255AFP647
Alexa Fluor Plus 680	NBP2-25255AFP680
Allophycocyanin	NBP2-25255APC
Biotin	NBP2-25255B
CoraFluor 1	NBP2-25255CL1
DyLight 350	NBP2-25255UV
DyLight 405	NBP2-25255V
DyLight 488	NBP2-25255G
DyLight 550	NBP2-25255R
DyLight 594	NBP2-25255DL594
DyLight 650	NBP2-25255C
DyLight 680	NBP2-25255FR
DyLight 755	NBP2-25255IR
FITC	NBP2-25255F
HRP	NBP2-25255H
Janelia Fluor 525	NBP2-25255JF525
Janelia Fluor 549	NBP2-25255JF549
Janelia Fluor 646	NBP2-25255JF646
Janelia Fluor 669	NBP2-25255JF669
PE	NBP2-25255PE
PerCP	NBP2-25255PCP
mFluor Violet 450 SE	NBP2-25255MFV450
mFluor Violet 500 SE	NBP2-25255MFV500
mFluor Violet 610 SE	NBP2-25255MFV610

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-25255SS
Azide and BSA Free	NBP2-80588

B7-1/CD80 Antibody [Unconjuga ...

AF740

HAF017). A specific band was detected for B7-1/CD80 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank"> Western Blot B7-1/CD80 Antibody [Unconjugated]

Western Blot B7-1/CD80 Antibody [Unconjugated]

F0107). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox" target="_blank"> Flow Cytometry B7-1/CD80 Antibody [Unconjugated]

Flow Cytometry B7-1/CD80 Antibody [Unconjugated]

AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107) and Rat Anti-Mouse B220/CD45R Alexa Fluor® 488-conjugated Monoclonal Antibody (FAB1217G). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox" target="_blank"> Flow Cytometry B7-1/CD80 Antibody [Unconjugated]

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox" target="_blank"> Immunocytochemistry B7-1/CD80 Antibody [Unconjugated]

Immunocytochemistry B7-1/CD80 Antibody [Unconjugated]

740-B1) co-stimulates IL-2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL-2 Quantikine ELISA Kit (Catalog # D2050). IL-2 secretion elicited by Recombinant Mouse B7-1/CD80 Fc Chimera (0.1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse B7-1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740). The ND₅₀ is typically 0.15-0.6 µg/mL." class="big_lightbox" target="_blank"> Neutralization B7-1/CD80 Antibody [Unconjugated]

Neutralization B7-1/CD80 Antibody [Unconjugated]

740-B1) was serially diluted and captured by Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank"> ELISA B7-1/CD80 Antibody [Unconjugated]

(11 Publications)

Species: Mu
Applications: WB, Flow, B/N, CyTOF-ready, ELISA(Cap), ICC/IF
Host: Goat Polyclonal

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF740U-100UG
Alexa Fluor 405	AF740V-100UG
Alexa Fluor 488	AF740G-100UG
Alexa Fluor 532	AF740X-100UG
Alexa Fluor 594	AF740T-100UG
Alexa Fluor 647	AF740R-100UG
Alexa Fluor 700	AF740N-100UG
Alexa Fluor 750	AF740S-100UG
Alexa Fluor Plus 405	AF740AFP405-100UG
Alexa Fluor Plus 488	AF740AFP488-100UG
Alexa Fluor Plus 555	AF740AFP555-100UG
Alexa Fluor Plus 594	AF740AFP594-100UG
Alexa Fluor Plus 647	AF740AFP647-100UG
Alexa Fluor Plus 680	AF740AFP680-100UG
Allophycocyanin	AF740A
Biotin	BAF740
CoraFluor 1	AF740CL1
DyLight 350	AF740D
DyLight 405	AF740E
DyLight 488	AF740K
DyLight 550	AF740L
DyLight 594	AF740M
DyLight 650	AF740W
DyLight 680	AF740Y
DyLight 755	AF740Z
FITC	AF740F
HRP	AF740H
Janelia Fluor 525	AF740JF525
Janelia Fluor 549	AF740I
Janelia Fluor 646	AF740J
Janelia Fluor 669	AF740JF669
PE	AF740P
PerCP	AF740C
mFluor Violet 450 SE	AF740MFV450
mFluor Violet 500 SE	AF740MFV500
mFluor Violet 610 SE	AF740MFV610

Formulation	Catalog #	Availability	Price
Carrier Free	AF740-SP

We have 54 B7-1/CD80 Antibodies

ELISA Kits

Mouse B7-1/CD80 DuoSet ELISA, ...

DY740

(1 Publication)

Species: Mu
Applications: ELISA

Human B7-1/CD80 DuoSet ELISA, ...

DY140

Species: Hu
Applications: ELISA

Mouse B7-1/CD80 ELISA Kit (Co ...

KA0991

(2 Publications)

Species: Mu
Applications: ELISA, NULL

We have 3 B7-1/CD80 ELISA Kits

Proteins

Recombinant Mouse B7-1/CD80 F ...

740-B1

(10 Publications)

Species: Mu
Applications: Bioactivity

Formulation	Catalog #	Availability	Price
Carrier Free	740-B1-100
Carrier Free	740-B1-01M

Recombinant Mouse B7-1/CD80 P ...

NBP2-26578

Bioactivity Recombinant Mouse B7-1/CD80 Protein

Species: Mu
Applications: PAGE, Cell Depl

Recombinant Human B7-1/CD80 F ...

10133-B1

AVI7268) was immobilized on a Biacore Sensor Chip CM5 via the Avi-tag biotin, and binding to Recombinant Human B7-1/CD80 Fc protein (Catalog # 10133-B1) was measured at a concentration range between 82 pM and 21 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.2511 nM." class="big_lightbox" target="_blank"> Surface Plasmon Resonance (SPR) B7-1/CD80 [Unconjugated]

Surface Plasmon Resonance (SPR) B7-1/CD80 [Unconjugated]

Species: Hu
Applications: Bioactivity

Formulation	Catalog #	Availability	Price
Carrier Free	10133-B1-100
Carrier Free	10133-B1-01M

We have 16 B7-1/CD80 Proteins

Description

The B-lymphocyte activation antigen B7-1 (CD80) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. After engagement of T-cell receptor with antigen in association with major histocompatibility complex class II, a second signal mediated through the binding of B7 to CD28 greatly upregulates the production of multiple lymphokines (1). The relatively small CTLA-4/B7-1 binding interface exhibits an unusually high degree of shape complementarity. CTLA-4 forms homodimers through a newly defined interface of highly conserved residues. This zipper-like oligomerization provides the structural basis for forming unusually stable signalling complexes at the T-cell surface, underscoring the importance of potent inhibitory signalling in human immune responses (2).

Bioinformatics

Entrez	12519 Mouse 25408 Rat 941 Human
Uniprot	AAH42665 Human NP_005182 Human NP_005182.1 Human O55202 Rat P33681 Human Q00609 Mouse
Product By Gene ID	941
Alternate Names	Activation B7-1 antigen B7 BB1 CD28LG1 CD28LGB7-1 antigen) CD80 antigen CD80 molecule costimulatory factor CD80 costimulatory molecule variant IgV-CD80 CTLA-4 counter-receptor B7.1 T-lymphocyte activation antigen CD80

Research Areas for B7-1/CD80

Find related products by research area and learn more about each of the different research areas below.

Adaptive Immunity
Cytokine Research
Immunology
Signal Transduction

Related B7-1/CD80 Blog Posts

Check out the latest blog posts on B7-1/CD80.

CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re... Read more.

B7-1/CD80 Products

Description

Bioinformatics

Research Areas for B7-1/CD80

Related B7-1/CD80 Blog Posts