B7-1/CD80 Antibody (62N3G8) [Alexa Fluor® 700]

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Flow Cytometry: B7-1/CD80 Antibody (62N3G8) [Alexa Fluor (R) 700] [NBP2-25255AF700] - analysis of CD80 in human PBMC (monocyte depleted) using anti-CD80 antibody. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
62N3G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700

B7-1/CD80 Antibody (62N3G8) [Alexa Fluor® 700] Summary

Immunogen
Amino acids 102-223 of human CD80 were used as the immunogen for the antibody.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD80
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-25255AF700 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for B7-1/CD80 Antibody (62N3G8) [Alexa Fluor® 700]

  • Activation B7-1 antigen
  • B7
  • B71
  • B7-1
  • BB1
  • CD28LG1
  • CD28LGB7-1 antigen)
  • CD80 antigen
  • CD80 molecule
  • CD80
  • costimulatory factor CD80
  • costimulatory molecule variant IgV-CD80
  • CTLA-4 counter-receptor B7.1
  • T-lymphocyte activation antigen CD80

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for B7-1/CD80 Antibody (NBP2-25255AF700) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NBP2-25255AF700:
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Flow
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry B7-1/CD80 NBP2-25255AF700
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reviewed by:
Anon ymous
Flow Human 06/03/2014
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Summary

ApplicationFlow Cytometry
Sample Testedhuman PBMC (monocyte depleted)
SpeciesHuman

Primary Anitbody

Dilution Ratio1/100, 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS

Details

Detection Notesflow cytometry
Wash Description2% human serum, 0.5 mM EDTA in DPBS, washed 2x after antibody staining

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for B7-1/CD80 Antibody (NBP2-25255AF700) (0)

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Secondary Antibodies

 

Isotype Controls

Additional B7-1/CD80 Products

Bioinformatics Tool for B7-1/CD80 Antibody (NBP2-25255AF700)

Discover related pathways, diseases and genes to B7-1/CD80 Antibody (NBP2-25255AF700). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for B7-1/CD80 Antibody (NBP2-25255AF700)

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Pathways for B7-1/CD80 Antibody (NBP2-25255AF700)

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PTMs for B7-1/CD80 Antibody (NBP2-25255AF700)

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Research Areas for B7-1/CD80 Antibody (NBP2-25255AF700)

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Blogs on B7-1/CD80.

CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.

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Recent Reviews

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Anon ymous
06/03/2014
Application: Flow
Species: Human

Bioinformatics

Gene Symbol CD80
Entrez