B220/CD45R Antibody (IVA103) - BSA Free

Immunocytochemistry: B220/CD45R Antibody (IVA103) [NB500-492] - Staining of acetone/methanol fixed bovine peripheral blood (lymphocyte-enriched suspension) using anti-bovine CD45R (IVA103); secondary antibody conjugated to FITC (green), DNA stained with DAPI (blue).

Surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD45R (IVA103) purified antibody (concentration in sample 0,1 ug/ml) GAM APC.

• Reactivity: Bv
• Applications: Flow, ICC/IF, IP, CyTOF-ready
• Clone: IVA103
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

B220/CD45R Antibody (IVA103) - BSA Free Summary

• Immunogen: Bovine leukocytes.
• Specificity: The antibody IVA103 reacts with CD45R antigen (bovine), a single chain type I transmembrane glycoprotein (member of the protein tyrosine phosphatase family), a restricted form of the CD45 family. CD45R is an essential regulator of T and B lymphocytes antigen receptor signaling and it is specifically expressed in hematopoietic cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1-4 ug/ml
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 1:50
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for B220/CD45R Antibody (IVA103) - BSA Free

• B220
• CD45 antigen
• CD45R
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

CD45R is a member of the protein tyrosine phosphatase (PTP) family and a major cell surface glycoprotein. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R represents a restricted form of the CD45 family which primarily recognizes only cells of B lineage from proB cell through mature B lymphocytes and, prior to the availability of anti CD19 MAbs, was commonly used as a pan B cell marker. It also reacts with certain activated T cells, as well as non MHC restricted lytically active lymphokine activated killer (LAK) cells. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T and B cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. CD45R also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRC