ATP5A Antibody

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Western Blot: ATP5A Antibody [NBP2-15512] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:2000. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: ATP5A Antibody [NBP2-15512] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term diluted at 1:500. ...read more
Immunohistochemistry-Frozen: ATP5A Antibody [NBP2-15512] - Frozen sectioned adult mouse retina. Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term diluted at 1:250. Red: Protein kinase C alpha ...read more
Western Blot: ATP5A Antibody [NBP2-15512] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:1000.
Western Blot: ATP5A Antibody [NBP2-15512] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody diluted by 1:2000.
Western Blot: ATP5A Antibody [NBP2-15512] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG ...read more
Western Blot: ATP5A Antibody [NBP2-15512] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term ...read more
Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Colon carcinoma. ATP5A1 antibody [C2C3], C-term dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Mouse stomach. ATP5A1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: ATP5A Antibody [NBP2-15512] - Mouse lung. ATP5A1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, RM, ZeSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ATP5A Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human ATP5A1. The exact sequence is proprietary.
Localization
Mitochondrion inner membrane
Marker
Mitochondria Marker
Predicted Species
Porcine (96%), Chimpanzee (100%), Bovine (96%), Rhesus Macaque (98%), Zebrafish (90%), Chicken (90%), Canine (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP5F1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-15512 in the following applications:

Reactivity Notes

Xenopus laevis (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for ATP5A Antibody

  • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1
  • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform1, cardiac muscle
  • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform2, non-cardiac muscle-like 2
  • ATP sythase (F1-ATPase) alpha subunit
  • ATP5AL2
  • ATP5AMOM2
  • ATPMATP synthase subunit alpha, mitochondrial
  • cardiac muscle
  • EC 3.6.3
  • EC 3.6.3.14
  • hATP1
  • mitochondrial ATP synthetase, oligomycin-resistant
  • OMR
  • ORMATP synthase alpha chain, mitochondrial

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the same protein have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, RM, Ze
Applications: WB, ICC/IF, IHC

Publications for ATP5A Antibody (NBP2-15512) (0)

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Review for ATP5A Antibody (NBP2-15512) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-15512:
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Images Ratings Applications Species Date Details
Western Blot ATP5A NBP2-15512
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5
reviewed by:
Verified Customer
WB Human 07/15/2023
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Summary

ApplicationWestern Blot
Sample Testedglioblastoma cell lysates
SpeciesHuman
Lot41871

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Verified Customer
07/15/2023
Application: WB
Species: Human

Bioinformatics

Gene Symbol ATP5F1A
Uniprot