CHD1 Antibody

Western Blot: CHD1 Antibody [NB100-60411] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and CHD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:2000 of Rabbit Anti-Human CHD1 Polyclonal Antibody (Catalog # NB100-60411) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CHD1 at approximately 260 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.

Western Blot: CHD1 Antibody [NB100-60411] - Detection of Human CHD1 on HeLa whole cell lysate using NB100-60411. CHD1 was also immunoprecipitated by rabbit anti-CHD1 antibody NB100-60410.

Chromatin Immunoprecipitation Sequencing: CHD1 Antibody [NB100-60411] - Localization of CHD1 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CHD1 antibody NB100-60411 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CHD1 binding within a 3.5 Mb region of the human X chromosome as detected using anti-CHD1 antibody NB100-60411. ChIP-seq validation performed by Diogenode, Denville, NJ.

Chromatin Immunoprecipitation: CHD1 Antibody [NB100-60411] - ChIP-chip scatter plot of anti-CHD1 (NB100-60411) enriched DNA binding sites versus input reference DNA. CHD1 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-CHD1 ChIP, normal rabbit IgG showed little enrichment.

Immunocytochemistry/ Immunofluorescence: CHD1 Antibody [NB100-60411] - Chromatin marks associated with transcriptional elongation are increased in the absence of Lsh. Western analysis for detection of Chd1 protein comparing whole cell extracts derived from Lsh-/-MEFs targeted by Chd1 siRNA or control siRNA. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0009163), licensed under a CC-BY license.

• Reactivity: Hu, Mu, Ch
• Applications: WB, IP, ChIP, KD, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CHD1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1660 and 1710 of human chromodomain helicase DNA binding protein 1 using the numbering given in entry NP_001261.2 (GeneID 1105).
• Predicted Species: Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1-5 ug/mg lysate
  • Chromatin Immunoprecipitation Sequencing
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1:2000-1:10000
• Application Notes: WB in human was reported in the following publication: PMID: 21880597

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 20161795).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CHD1 Antibody

• ATP-dependent helicase CHD1
• CHD1
• CHD-1
• chromodomain helicase DNA binding protein 1
• chromodomain-helicase-DNA-binding protein 1
• DKFZp686E2337
• EC 3.6.1
• EC 3.6.4.12

Background

The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHD1 Antibody (NB100-60411)(7)

Publications using NB100-60411

• Kareddula A, Medina DJ, Petrosky W et al. The role of chromodomain helicase DNA binding protein 1 (CHD1) in promoting an invasive prostate cancer phenotype Ther Adv Urol 2021-08-04 [PMID: 34408788] (Chemotaxis, Western Blot, Mouse)
• Shahin Varnoosfaderani F, Palau A, Dong W et al. A regulatory role for CHD2 in myelopoiesis Epigenetics 2020-01-10 [PMID: 31900031] (WB, Human)
• Cheng LC, Tan VM, Ganesan S, Drake JM. Integrating phosphoproteomics into the clinical management of prostate cancer. Clin Transl Med 2017-12-01 [PMID: 28197968] (IP, Human)
• Huang S, Gulzar ZG, Salari K et al. Recurrent deletion of CHD1 in prostate cancer with relevance to cell invasiveness. Oncogene 2012-09-13 [PMID: 22179824] (Human)
• Liu W, Lindberg J, Sui G et al. Identification of novel CHD1-associated collaborative alterations of genomic structure and functional assessment of CHD1 in prostate cancer. Oncogene 2012-08-01 [PMID: 22139082]
• Tao Y, Liu S, Briones V et al. Treatment of breast cancer cells with DNA demethylating agents leads to a release of Pol II stalling at genes with DNA-hypermethylated regions upstream of TSS. Nucleic Acids Res. 2011-12-01 [PMID: 21880597] (WB, Human)
• Tao Y, Xi S, Briones V et al. Lsh mediated RNA polymerase II stalling at HoxC6 and HoxC8 involves DNA methylation. PLoS One. 2010-02-11 [PMID: 20161795] (Chemotaxis, WB, Mouse)

Bioinformatics

• Gene Symbol: CHD1
• Entrez:
  • 1105(Human)
• Uniprot:
  • O14646(Human)