CHD1 Antibody

Images

 
Genetic Strategies: Knockout Validated: CHD1 Antibody [NB100-60411] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and CHD1 knockout (KO) HEK293T cell line. PVDF membrane was ...read more
Western Blot: CHD1 Antibody [NB100-60411] - Detection of Human CHD1 on HeLa whole cell lysate using NB100-60411. CHD1 was also immunoprecipitated by rabbit anti-CHD1 antibody NB100-60410.
Chromatin Immunoprecipitation Sequencing: CHD1 Antibody [NB100-60411] - Localization of CHD1 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CHD1 antibody NB100-60411 and ...read more
Chromatin Immunoprecipitation: CHD1 Antibody [NB100-60411] - ChIP-chip scatter plot of anti-CHD1 (NB100-60411) enriched DNA binding sites versus input reference DNA. CHD1 was used to immunoprecipitate chromatin from ...read more
Genetic Strategies: Knockdown Validated: CHD1 Antibody [NB100-60411] - Chromatin marks associated with transcriptional elongation are increased in the absence of Lsh. Western analysis for detection of Chd1 ...read more
Chromatin marks associated with transcriptional elongation are increased in the absence of Lsh. (G) Western analysis for detection of Chd1 protein comparing whole cell extracts derived from Lsh-/-MEFs targeted by Chd1 ...read more

Product Details

Summary
Reactivity Hu, Mu, ChSpecies Glossary
Applications WB, IP, ChIP, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

CHD1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1660 and 1710 of human chromodomain helicase DNA binding protein 1 using the numbering given in entry NP_001261.2 (GeneID 1105).
Predicted Species
Chicken (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1-5 ug/mg lysate
  • Chromatin Immunoprecipitation Sequencing
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1:2000-1:10000
Application Notes
WB in human was reported in the following publication: PMID: 21880597
Publications
Read Publications using
NB100-60411 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 20161795).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CHD1 Antibody

  • ATP-dependent helicase CHD1
  • CHD1
  • CHD-1
  • chromodomain helicase DNA binding protein 1
  • chromodomain-helicase-DNA-binding protein 1
  • DKFZp686E2337
  • EC 3.6.1
  • EC 3.6.4.12

Background

The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHD1 Antibody (NB100-60411)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Chemotaxis, IP, WB.


Filter By Application
Chemotaxis
(1)
IP
(1)
WB
(4)
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Filter By Species
Human
(4)
Mouse
(1)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional CHD1 Products

Array NB100-60411

Research Areas for CHD1 Antibody (NB100-60411)

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Blogs on CHD1

There are no specific blogs for CHD1, but you can read our latest blog posts.
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Bioinformatics

Gene Symbol CHD1
Entrez
Uniprot