CHD1 Antibody

Images

 
Genetic Strategies: Knockdown Validated: CHD1 Antibody [NB100-60411] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and CHD1 knockout (KO) HEK293T cell line. PVDF membrane was ...read more
Independent Antibodies: Western Blot: CHD1 Antibody [NB100-60411] - Detection of Human CHD1 on HeLa whole cell lysate using NB100-60411. CHD1 was also immunoprecipitated by rabbit anti-CHD1 antibody NB100-60410.
Chromatin Immunoprecipitation Sequencing: CHD1 Antibody [NB100-60411] - Localization of CHD1 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CHD1 antibody NB100-60411 and ...read more
Chromatin Immunoprecipitation: CHD1 Antibody [NB100-60411] - ChIP-chip scatter plot of anti-CHD1 (NB100-60411) enriched DNA binding sites versus input reference DNA. CHD1 was used to immunoprecipitate chromatin from ...read more

Product Details

Summary
Reactivity Hu, Mu, ChSpecies Glossary
Applications WB, ChIP, IP, CHIP-SEQ, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

CHD1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1660 and 1710 of human chromodomain helicase DNA binding protein 1 using the numbering given in entry NP_001261.2 (GeneID 1105).
Predicted Species
Chicken (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation 1-5 ug/mg lysate
  • Immunoprecipitation 2-5 ug/mg lysate
  • Chromatin Immunoprecipitation Sequencing
  • Knockdown Validated
Application Notes
WB in human was reported in the following publication: PMID: 21880597
Control
CHD1 Knockout 293T Cell Lysate
Publications
Read Publications using
NB100-60411 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    3 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 20161795).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CHD1 Antibody

  • ATP-dependent helicase CHD1
  • CHD1
  • CHD-1
  • chromodomain helicase DNA binding protein 1
  • chromodomain-helicase-DNA-binding protein 1
  • DKFZp686E2337
  • EC 3.6.1
  • EC 3.6.4.12

Background

The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CHD1 Antibody (NB100-60411)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ChIP, IP, WB.


Filter By Application
ChIP
(1)
IP
(1)
WB
(3)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for CHD1 Antibody (NB100-60411) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol CHD1
Entrez
Uniprot