alpha 1-Acid Glycoprotein Antibody (386131) [Unconjugated]

Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human a1-Acid Glycoprotein Monoclonal Antibody (Catalog # MAB3694) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for a1-Acid Glycoprotein at approximately 40-50 kDa (as indicated). This experiment was conducted under reducing and non-reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of human plasma. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human a1-Acid Glycoprotein Monoclonal Antibody (Catalog # MAB3694) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a1-Acid Glycoprotein at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

a1-Acid Glycoprotein was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human a1-Acid Glycoprotein Monoclonal Antibody (Catalog # MAB3694) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 386131
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

alpha 1-Acid Glycoprotein Antibody (386131) [Unconjugated] Summary

• Immunogen: Human plasma-derived alpha 1-Acid Glycoprotein
  Accession # P02763
• Specificity: Detects human alpha 1‑Acid Glycoprotein in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ORM1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha 1-Acid Glycoprotein Antibody (386131) [Unconjugated]

• AGP 1
• AGP
• AGP1
• AGP1alpha-1-acid glycoprotein 1
• AGPA
• AGP-A
• alpha 1Acid Glycoprotein
• alpha 1-Acid Glycoprotein
• OMD 1
• ORM
• ORM1
• orosomucoid 1
• Orosomucoid
• orosomucoid-1

Background

alpha 1-Acid Glycoprotein (AGP-1), also known as orosomucoid-1, is an acute phase protein secreted by the liver. Serum AGP-1 levels are elevated during inflammatory responses. AGP-1 binds a wide range of molecules in the circulation. Human and mouse AGP-1 share 43% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha 1-Acid Glycoprotein Antibody (MAB3694)(4)

Publications using MAB3694

• JH Park, J Reunert, M He, RG Mealer, M Noel, Y Wada, M Grüneberg, J Horváth, RD Cummings, O Schwartz, T Marquardt L-Fucose treatment of FUT8-CDG Molecular genetics and metabolism reports, 2020-12-05;25(0):100680. 2020-12-05 [PMID: 33312876] (Western Blot, Human)
• Si-Chang Qu, Ding Xu, Ting-Ting Li, Jing-Fa Zhang, Fang Liu iTRAQ-based proteomics analysis of aqueous humor in patients with dry age-related macular degeneration International Journal of Ophthalmology 2019-11-18 [PMID: 31741866]
• Wenjuan Wu, Qi Wang, Fuqiang Yin, Zhijun Yang, Wei Zhang, Hani Gabra et al. Identification of proteomic and metabolic signatures associated with chemoresistance of human epithelial ovarian cancer International Journal of Oncology 2016-10-01 [PMID: 27511453]
• Tao Fang, Meiling Cui, Ji Sun, Chao Ge, Fangyu Zhao, Lin Zhang et al. Orosomucoid 2 inhibits tumor metastasis and is upregulated by CCAAT/enhancer binding protein beta in hepatocellular carcinomas Oncotarget 2015-04-19 [PMID: 25965830]

Bioinformatics

• Gene Symbol: ORM1
• Uniprot:
  • P02763()