ATM Antibody

Images

 
Western Blot: ATM Antibody [NB100-104] - Detection of ATM in HeLa nuclear extract using NB100-104. (30 sec. exposure)
Immunocytochemistry/ Immunofluorescence: ATM Antibody [NB100-104] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.05% Triton-X100. Then cells were ...read more
Immunohistochemistry: ATM Antibody [NB100-104] - Staining of Testis, Seminiferous Tubule.
Flow Cytometry: ATM Antibody [NB100-104] - Analysis of PE conjugate of NB100-104. An intracellular stain was performed on HeLa cells with ATM antibody NB100-104PE (blue) and a matched isotype control NBP2-24893PE ...read more
Western Blot: ATM Antibody [NB100-104] - Detection of ATM in Raji whole cell lysate.
Immunocytochemistry/ Immunofluorescence: ATM Antibody [NB100-104]

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2.5 mg/ml

ATM Antibody Summary

Immunogen
A fragment of the human ATM protein corresponding to the C-terminus (within the last third of the protein sequence). [UniProt# Q13315]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This ATM antibody can be used for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, it detects a band at ~350 kDa, representing ATM. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in immunoblotting reported in scientific literature (PMID 28512243).
Theoretical MW
350 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-104 in the following applications:

Publications
Read Publications using
NB100-104 in the following applications:

  • 3 publications
  • 2 publications
  • 1 publication
  • IB
    1 publication
  • 12 publications
  • IHC
    3 publications
  • IP
    6 publications
  • WB
    56 publications

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
2.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATM Antibody

  • AT mutated
  • A-T mutated
  • AT1
  • ATA
  • ataxia telangiectasia mutated (includes complementation groups A, C and D)
  • ataxia telangiectasia mutatedATD
  • ATC
  • ATDC
  • ATE
  • ATM
  • DKFZp781A0353
  • EC 2.7.11.1
  • MGC74674
  • serine-protein kinase ATM
  • TEL1
  • TEL1, telomere maintenance 1, homolog
  • TELO1
  • TPLL

Background

ATM (ataxia telangiectasia mutated kinase), the master regulator of DNA double-strand breaks (DSBs) repair pathway, is a serine/threonine protein kinase that act as DNA damage sensor by activating checkpoint signaling upon DSBs, apoptosis and genotoxic stresses. ATM activation involves its recruitment to DSBs through interaction with the MRE11-RAD50-NBS1 or MRN complex, followed by KAT5/TIP60 mediated acetylation. ATM recognizes the substrate consensus sequence [ST]-Q and phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at DSBs, thereby regulating DNA damage response mechanism. ATM also implicates in vesicle and/or protein transport, T-cell development, gonads/neurological function, pre-B cell allelic exclusion, signal transduction, cell cycle control and act as a tumor suppressor. ATM exist as dimers or tetramers in inactive state and on DNA damage, autophosphorylation dissociates ATM into monomers rendering them catalytically active and binds/activates ABL1, SAPK, DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. ATM is an integral part of BASC complex (BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex) and interacts with DCLRE1C, KAT8, KAT5, NABP2, ATMIN, CEP164, AP2B1, AP3B2, TELO2, TTI1, DDX1 etc. NUAK1/ARK5 mediated ATM phosphorylation and ATM autophosphorylation at Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-regulated activation of the kinase. Defects in ATM are the cause of ataxia telangiectasia (AT), T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL), B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL), B-cell chronic lymphocytic leukemia (BCLL).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATM Antibody (NB100-104)(78)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: ELISA, FLOW, Flow, IB, ICC/IF, IHC, IP, WB.


Filter By Application
ELISA
(3)
FLOW
(2)
Flow
(1)
IB
(1)
ICC/IF
(12)
IHC
(3)
IP
(6)
WB
(56)
All Applications
Filter By Species
Human
(54)
Mouse
(9)
All Species
Showing Publications 1 - 10 of 78. Show All 78 Publications.
Publications using NB100-104 Applications Species
Wu PK, Wang JY, Chen CF et al. Early Passage Mesenchymal Stem Cells Display Decreased Radiosensitivity and Increased DNA Repair Activity. Stem Cells Transl Med Jun 1 2017 [PMID: 28544661] (WB, Human) WB Human
Li Z, Li J, Kong Y et al. Plk1 Phosphorylation of Mre11 Antagonizes the DNA Damage Response. Cancer Res. May 16 2017 [PMID: 28512243] (IB, Human) IB Human
Qi Y, Qiu Q, Gu X et al. ATM mediates spermidine-induced mitophagy via PINK1 and Parkin regulation in human fibroblasts. Sci Rep. 2016 Apr 19 [PMID: 27089984] (ICC/IF, Human) ICC/IF Human
Frum RA, Love IM, Damle PK et al. Constitutive Activation of DNA Damage Checkpoint Signaling Contributes to Mutant p53 Accumulation via Modulation of p53 Ubiquitination. Mol. Cancer Res. May 1 2016 [PMID: 26965143] (Flow, ICC/IF, Human) Flow, ICC/IF Human
Nagy Z, Kalousi A, Furst A et al. Tankyrases Promote Homologous Recombination and Check Point Activation in Response to DSBs. PLoS Genet 2016 Feb [PMID: 26845027] (WB) WB
Murata Y, Uehara Y, Hosoi Y. Activation of mTORC1 under nutrient starvation conditions increases cellular radiosensitivity in human liver cancer cell lines, HepG2 and HuH6. Biochem. Biophys. Res. Commun. 2015 Nov 13 [PMID: 26585486] (WB, Human) WB Human
Parplys AC, Zhao W, Sharma N et al. NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability. Nucleic Acids Res. 2015 Aug 31 [PMID: 26323318] (WB, Human) WB Human
Silva Barbara Alcaraz, Stambaugh Jessica R, Yokomori Kyoko et al. DNA damage to a single chromosome end delays anaphase onset. J Biol Chem. 2014 Aug 15 [PMID: 24982423]
Morita A, Tanimoto K, Murakami T et al. Mitochondria are required for ATM activation by extranuclear oxidative stress in cultured human hepatoblastoma cell line Hep G2 cells. Biochem Biophys Res Commun 2014 Jan 6 [PMID: 24406161] (WB, Human) WB Human
Yoon JY, Ishdorj G, Graham BA, Gibson SB. Valproic acid enhances fludarabine-induced apoptosis mediated by ROS and involving decreased AKT and ATM activation in B-cell-lymphoid neoplastic cells. Apoptosis 2014 Jan 1 [PMID: 24057147] (WB, Human) WB Human
Show All 78 Publications.

Reviews for ATM Antibody (NB100-104) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.
We have 2 reviews tested in 2 application: IF, WB.

Reviews using NB100-104:
Filter by Applications
IF
(1)
WB
(1)
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Filter by Species
Human
(1)
Other
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Barbara Alcaraz Silva
IF Other 10/08/2014
View

Summary

ApplicationImmunofluorescence
Sample Testedcell
SpeciesOther
LotN1

Details

Fixation Details4% PFA, RT
Western Blot ATM NB100-104
Enlarge
reviewed by:
Christie Brown
WB Human 08/15/2013
View

Summary

ApplicationWestern Blot
Sample TestedLymphoblastoid (LCL) nuclear lysate
SpeciesHuman
LotP-1
CommentsThis ATM antibody is very clean and specific. It does not show an ATM band in lymphoblastoid cell lines from A-T patients. It usually requires a 5-10 min exposure. I get better results from nuclear lysates than from whole cell lysates.

Blocking

Blocking Details5% milk in PBSt

Primary Anitbody

Dilution Ratio1/1000, overnight, 5% milk in PBSt

Secondary Antibody

Secondary Description5% milk in PBSt
Secondary Manufacturer Cat#45-000-682
Secondary Concentration1/3000

Details

Detection NotesAmersham ECL, 10min

Comments

CommentsThis ATM antibody is very clean and specific. It does not show an ATM band in lymphoblastoid cell lines from A-T patients. It usually requires a 5-10 min exposure. I get better results from nuclear lysates than from whole cell lysates.

Product General Protocols

View specific protocols for ATM Antibody (NB100-104): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATM Antibody (NB100-104). (Showing 1 - 1 of 1 FAQs).

  1. What is the expiration date of this product?
    • We guarantee the stability of this product for 1 year from the date of receipt.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-104AF405
Alexa Fluor 488 Labeled NB100-104AF488
Alexa Fluor 647 Labeled NB100-104AF647
Alexa Fluor 700 Labeled NB100-104AF700
Biotin Labeled NB100-104B
DyLight 405 Labeled NB100-104V
DyLight 488 Labeled NB100-104G
DyLight 550 Labeled NB100-104R
DyLight 650 Labeled NB100-104C
FITC Labeled NB100-104F
HRP Labeled NB100-104H
PE Labeled NB100-104PE

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Blogs on ATM.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

ATM and DSB Repair in Cancer
Ataxia Telangiectasia Mutated (ATM) is a serine/threonine protein kinase that is the master regulator of the DNA double-strand break (DSB) repair pathway. ATM is a key part of the cell cycle machinery that activates checkpoint signaling in response to...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Barbara Alcaraz Silva
10/08/2014
Application: IF
Species: Other

Christie Brown
08/15/2013
Application: WB
Species: Human

Bioinformatics

Gene Symbol ATM
Entrez
Uniprot