ATM Antibody


Western Blot: ATM Antibody [NB100-104] - Detection of ATM in HeLa nuclear extract using ATM antibody [NB100-104]. Theoretical molecular weight 351 kDa.
Immunohistochemistry-Paraffin: ATM Antibody [NB100-104] - Staining of human tonsil, germinal center and mantle zone with ATM Antibody [NB100-104].
Biological Strategies: Immunocytochemistry/ Immunofluorescence: ATM Antibody [NB100-104] - GM00637 cells with KU55933 pretreatment (a) were exposed to 50 uM spermidine or CCCP, followed by immunofluorescence more
Flow Cytometry: ATM Antibody [NB100-104] - An intracellular stain was performed on HeLa cells with Dylight 550-conjugated ATM Antibody [NB100-104R] (blue) and a matched isotype control (orange). Cells were fixed with 4% more
Biological Strategies: Western Blot: ATM Antibody [NB100-104] - Analysis of double strand break repair in MCF-7 monolayer and mammosphere populations. Expression of proteins involved in the NHEJ pathway of DSB more
Western Blot: ATM Antibody [NB100-104] - Detection of ATM in Raji whole cell lysate using [NB100-104]. Observed molecular weight ~300 kDa. Theoretical molecular weight 351 kDa.
Immunocytochemistry/ Immunofluorescence: ATM Antibody [NB100-104] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.05% Triton X-100. Then cells were more
Flow Cytometry: ATM Antibody [NB100-104] - Analysis of PE conjugate of ATM Antibody [NB100-104PE]. An intracellular stain was performed on HeLa cells with ATM antibody [NB100-104PE] (blue) and a matched isotype control more
Flow Cytometry: ATM Antibody [NB100-104] - An intracellular stain was performed on HepG2 cells with PE-conjugated ATM antibody [NB100-104PE] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA more
Depletion of SMARCAL1 induces DNA damage and checkpoint activation in iSML1 iPSCs. (C) Immunoblot detection of the indicated DDR proteins in iSML1 iPSCs after 14 days of continuous treatment with DOX. Lamin B1 was used more
Osteopontin (OPN) is weakly expressed in NHF, HUVSMC and HUVEC and is not induced in hypoxia. A, Levels of OPN mRNA in AVF patients (n = 12) compared to control veins (Ctl). ****, P < .0001 (Test Mann‐Whitney). B, more
TRF1HP1 alpha results in reduced TIFs induced by mutant hTR expression. Cells stably expressing TRF1HP1 alpha (WT or mutant variants of HP1 alpha ) were infected with lentivirus containing WT hTR, or mutant hTR (47A more

Product Details

Reactivity Hu, Mu, Rt, Ca, MaSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, WB
2.5 mg/ml
Validated by:

Biological Strategies


ATM Antibody Summary

ATM Antibody was made to a fragment of the human ATM protein corresponding to the C-terminus (within the last third of the protein sequence). [Uniprot: Q13315]
Immunogen affinity purified
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  • Flow Cytometry
  • Immunoblotting reported in scientific literature (PMID 28512243)
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500-1:1000
Application Notes
In Western blot, it detects a band at ~350 kDa, representing ATM.
Theoretical MW
351 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
NB100-104 in the following applications:

Read Publications using
NB100-104 in the following applications:

  • B/N
    1 publication
  • 3 publications
  • 3 publications
  • IB
    1 publication
  • 14 publications
  • 5 publications
  • IP
    6 publications
  • WB
    72 publications

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 31648115).

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS, 50% Glycerol
0.05% Sodium Azide
2.5 mg/ml
Immunogen affinity purified

Alternate Names for ATM Antibody

  • AT mutated
  • A-T mutated
  • AT1
  • ATA
  • ataxia telangiectasia mutated (includes complementation groups A, C and D)
  • ataxia telangiectasia mutated
  • ATC
  • ATD
  • ATDC
  • ATE
  • ATM serine/threonine kinase
  • ATM
  • DKFZp781A0353
  • EC
  • MGC74674
  • serine-protein kinase ATM
  • TEL1
  • TEL1, telomere maintenance 1, homolog
  • TELO1
  • TPLL


ATM (ataxia telangiectasia mutated kinase) is the master regulator of the DNA double-strand break (DSB) repair pathway. This ubiquitously expressed serine/threonine protein kinase belongs to the PI3K-like family of proteins and responds to DSBs caused by oxidative and other genotoxic stresses (1). In addition to regulating the DNA damage response, ATM participates in vesicle and protein transport, T-cell development, gonads/neurological function, pre-B cell allelic exclusion, cell cycle control, and acts as a tumor suppressor (2,3). Defects in ATM are associated with ataxia telangiectasia (AT), T-cell acute lymphoblastic leukemia (TALL), T-prolymphocytic leukemia (TPLL), and B-cell non-Hodgkin lymphomas (BNHL) including mantle cell lymphoma (MCL) and B-cell chronic lymphocytic leukemia (BCLL) (4).

The theoretical molecular weight of ATM is 350 kDa and it has 3 main domains: a FAT (focal adhesion targeting) domain (aa 1960-2566), a PI-3/PI-4 kinase catalytic domain (aa 2712-2962), and a C-terminal FAT domain (aa 3024-3056). ATM exists as a dimer or tetramer in its inactive state. Upon sensing DNA damage, the MRE11-RAD50-NBS1 (MRN) complex recruits ATM. The intricate process of ATM activation involves acetylation by KAT5/TIP60, autophosphorylation at Ser-1981, and dissociation into catalytically active monomers (5). Following activation, ATM phosphorylates multiple substrates such as p53/TP53 and Chk2 involved in DNA repair, checkpoint signaling, and the apoptosis pathway.


1. Paull TT. (2015) Mechanisms of ATM Activation. Annu Rev Biochem. 84:711-38. PMID: 25580527

2. Chaudhary MW and Al-Baradie RS. (2014) Ataxia-telangiectasia: future prospects. Appl Clin Genet. 7:159-167. PMID: 25258552

3. Stagni V, Cirotti C, and Barila D. (2018) Ataxia-Telangiectasia Mutated Kinase in the Control of Oxidative Stress, Mitochondria, and Autophagy in Cancer: A Maestro With a Large Orchestra. Front Oncol. 8:73. PMID: 29616191

4. Gumy-Pause F, Wacker P, and Sappino AP. (2004) ATM gene and lymphoid malignancies. Leukemia. 18(2):238-42. PMID: 14628072

5. Adamowicz M. (2018) Breaking up with ATM. J Immunol Sci. 2(1):26-31. PMID: 29652413


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATM Antibody (NB100-104)(99)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 8 applications: B/N, ELISA, FLOW, IB, ICC/IF, IF/IHC, IP, WB.

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Showing Publications 1 - 10 of 99. Show All 99 Publications.
Publications using NB100-104 Applications Species
Urushihara Y, Hashimoto T, Fujishima Y, Hosoi Y. AMPK/FOXO3a Pathway Increases Activity and/or Expression of ATM, DNA-PKcs, Src, EGFR, PDK1, and SOD2 and Induces Radioresistance under Nutrient Starvation International Journal of Molecular Sciences 2023-08-15 [PMID: 37629008] (WB) WB
Hashimoto T, Urushihara Y, Murata Y et al. AMPK increases expression of ATM through transcriptional factor Sp1 and induces radioresistance under severe hypoxia in glioblastoma cell lines Biochemical and biophysical research communications 2021-12-23 [PMID: 34973534] (WB, Human) WB Human
Roulston A, Zimmermann M, Papp R et al. RP-3500: A Novel, Potent, and Selective ATR Inhibitor that is Effective in Preclinical Models as a Monotherapy and in Combination with PARP Inhibitors Molecular Cancer Therapeutics 2022-02-01 [PMID: 34911817] (B/N, WB) B/N, WB
Sato H, Singer RH Cellular variability of nonsense-mediated mRNA decay Nature communications 2021-12-10 [PMID: 34893608] (ICC/IF, Human) ICC/IF Human
Nishiyama Y, Morita A, Tatsuta S Et al. Isorhamnetin Promotes 53BP1 Recruitment through the Enhancement of ATM Phosphorylation and Protects Mice from Radiation Gastrointestinal Syndrome Genes 2021-09-26 [PMID: 34680909] (WB, Human) WB Human
Chakraborty P, Hiom K DHX9-dependent recruitment of BRCA1 to RNA promotes DNA end resection in homologous recombination Nature communications 2021-07-05 [PMID: 34226554] (WB) WB
Gupta M, Liu X, Teraoka SN et al. Genes affecting ionizing radiation survival identified through combined exome sequencing and functional screening Human mutation 2021-06-21 [PMID: 34153142]
Zhang JQJ, Saravanabavan S, Chandra AN et al. Up-regulation of DNA Damage Response Signaling in Autosomal Dominant Polycystic Kidney Disease The American journal of pathology 2021-02-04 [PMID: 33549515]
Chakravarti D, Hu B, Mao X et al. Telomere dysfunction activates YAP1 to drive tissue inflammation Nat Commun 2020-09-21 [PMID: 32958778] (WB, Mouse) WB Mouse
Habib R, Kim R, Neitzel H et al. Telomere attrition and dysfunction: a potential trigger of the progeroid phenotype in nijmegen breakage syndrome Aging (Albany NY) 2020-06-22 [PMID: 32564008]
Show All 99 Publications.

Reviews for ATM Antibody (NB100-104) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.

Reviews using NB100-104:
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Images Ratings Applications Species Date Details
reviewed by:
Barbara Alcaraz Silva
IF Other 10/08/2014


Sample Testedcell
Western Blot ATM NB100-104
reviewed by:
Christie Brown
WB Human 08/15/2013


ApplicationWestern Blot
Sample TestedLymphoblastoid (LCL) nuclear lysate

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATM Antibody (NB100-104). (Showing 1 - 4 of 4 FAQs).

  1. What is the theoretical molecular weight for your ATM antibodies?
    • The theoretical molecular weight for our ATM antibodies is 351 kDa.
  2. What is the expiration date of this product?
    • We guarantee the stability of this product for 1 year from the date of receipt.
  3. What is the isotype of this antibody?
    • The isotype for this ATM Antibody is IgG.
  4. Which applications can I use this antibody in?
    • This antibody has been cited in multiple publications for use in ELISA, flow cytometry, immunoblotting, ICC/IF, immunohistochemistry, immunoprecipitation, and western blot. This antibody has also been tested in IHC-Paraffin; therefore all of these applications fall under our 100% guarantee. If you would like to test this antibody in an application or species not listed, please check out our Innovator's Reward Program.

Secondary Antibodies


Isotype Controls

Additional ATM Products

Research Areas for ATM Antibody (NB100-104)

Find related products by research area.

Blogs on ATM.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels ...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

ATM and DSB Repair in Cancer
Ataxia Telangiectasia Mutated (ATM) is a serine/threonine protein kinase that is the master regulator of the DNA double-strand break (DSB) repair pathway. ATM is a key part of the cell cycle machinery that activates checkpoint signaling in response to...  Read full blog post.

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Recent Reviews


Barbara Alcaraz Silva
Application: IF
Species: Other

Christie Brown
Application: WB
Species: Human


Gene Symbol ATM