ATM Antibody


Western Blot: ATM Antibody [NB100-104] - Detection of ATM in HeLa nuclear extract using ATM antibody [NB100-104]. Theoretical molecular weight 351 kDa.
Immunohistochemistry-Paraffin: ATM Antibody [NB100-104] - Staining of human tonsil, germinal center and mantle zone with ATM Antibody [NB100-104].
Immunocytochemistry/ Immunofluorescence: ATM Antibody [NB100-104] - GM00637 cells with KU55933 pretreatment (a) were exposed to 50 uM spermidine or CCCP, followed by immunofluorescence analyses of total and p-ATM on more
Flow Cytometry: ATM Antibody [NB100-104] - An intracellular stain was performed on HeLa cells with Dylight 550-conjugated ATM Antibody [NB100-104R] (blue) and a matched isotype control (orange). Cells were fixed with 4% more
Western Blot: ATM Antibody [NB100-104] - Analysis of double strand break repair in MCF-7 monolayer and mammosphere populations. Expression of proteins involved in the NHEJ pathway of DSB repair in response to increasing more
Western Blot: ATM Antibody [NB100-104] - Detection of ATM in Raji whole cell lysate using [NB100-104]. Observed molecular weight ~300 kDa. Theoretical molecular weight 351 kDa.
Immunocytochemistry/ Immunofluorescence: ATM Antibody [NB100-104] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.05% Triton X-100. Then cells were more
Flow Cytometry: ATM Antibody [NB100-104] - Analysis of PE conjugate of ATM Antibody [NB100-104PE]. An intracellular stain was performed on HeLa cells with ATM antibody [NB100-104PE] (blue) and a matched isotype control more
Flow Cytometry: ATM Antibody [NB100-104] - An intracellular stain was performed on HepG2 cells with PE-conjugated ATM antibody [NB100-104PE] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA more

Product Details

Reactivity Hu, Mu, Rt, Ca, MaSpecies Glossary
Applications WB, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP
2.5 mg/ml

ATM Antibody Summary

ATM Antibody was made to a fragment of the human ATM protein corresponding to the C-terminus (within the last third of the protein sequence). [Uniprot: Q13315]
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:500-1:1000
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
In Western blot, it detects a band at ~350 kDa, representing ATM. Use in immunoblotting reported in scientific literature (PMID: 28512243).
Theoretical MW
351 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
NB100-104 in the following applications:

Read Publications using
NB100-104 in the following applications:

  • 3 publications
  • 2 publications
  • 1 publication
  • IB
    1 publication
  • 13 publications
  • IHC
    5 publications
  • IP
    7 publications
  • WB
    66 publications

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 31648115).

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS, 50% Glycerol
0.05% Sodium Azide
2.5 mg/ml
Immunogen affinity purified

Alternate Names for ATM Antibody

  • AT mutated
  • A-T mutated
  • AT1
  • ATA
  • ataxia telangiectasia mutated (includes complementation groups A, C and D)
  • ataxia telangiectasia mutated
  • ATC
  • ATD
  • ATDC
  • ATE
  • ATM serine/threonine kinase
  • ATM
  • DKFZp781A0353
  • EC
  • MGC74674
  • serine-protein kinase ATM
  • TEL1
  • TEL1, telomere maintenance 1, homolog
  • TELO1
  • TPLL


ATM (ataxia telangiectasia mutated kinase) is the master regulator of the DNA double-strand break (DSB) repair pathway. This ubiquitously expressed serine/threonine protein kinase belongs to the PI3K-like family of proteins and responds to DSBs caused by oxidative and other genotoxic stresses (1). In addition to regulating the DNA damage response, ATM participates in vesicle and protein transport, T-cell development, gonads/neurological function, pre-B cell allelic exclusion, cell cycle control, and acts as a tumor suppressor (2,3). Defects in ATM are associated with ataxia telangiectasia (AT), T-cell acute lymphoblastic leukemia (TALL), T-prolymphocytic leukemia (TPLL), and B-cell non-Hodgkin lymphomas (BNHL) including mantle cell lymphoma (MCL) and B-cell chronic lymphocytic leukemia (BCLL) (4).

The theoretical molecular weight of ATM is 350 kDa and it has 3 main domains: a FAT (focal adhesion targeting) domain (aa 1960-2566), a PI-3/PI-4 kinase catalytic domain (aa 2712-2962), and a C-terminal FAT domain (aa 3024-3056). ATM exists as a dimer or tetramer in its inactive state. Upon sensing DNA damage, the MRE11-RAD50-NBS1 (MRN) complex recruits ATM. The intricate process of ATM activation involves acetylation by KAT5/TIP60, autophosphorylation at Ser-1981, and dissociation into catalytically active monomers (5). Following activation, ATM phosphorylates multiple substrates such as p53/TP53 and Chk2 involved in DNA repair, checkpoint signaling, and the apoptosis pathway.


1. Paull TT. (2015) Mechanisms of ATM Activation. Annu Rev Biochem. 84:711-38. PMID: 25580527

2. Chaudhary MW and Al-Baradie RS. (2014) Ataxia-telangiectasia: future prospects. Appl Clin Genet. 7:159-167. PMID: 25258552

3. Stagni V, Cirotti C, and Barila D. (2018) Ataxia-Telangiectasia Mutated Kinase in the Control of Oxidative Stress, Mitochondria, and Autophagy in Cancer: A Maestro With a Large Orchestra. Front Oncol. 8:73. PMID: 29616191

4. Gumy-Pause F, Wacker P, and Sappino AP. (2004) ATM gene and lymphoid malignancies. Leukemia. 18(2):238-42. PMID: 14628072

5. Adamowicz M. (2018) Breaking up with ATM. J Immunol Sci. 2(1):26-31. PMID: 29652413


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATM Antibody (NB100-104)(90)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 8 applications: ELISA, FLOW, Flow, IB, ICC/IF, IHC, IP, WB.

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Showing Publications 1 - 10 of 90. Show All 90 Publications.
Publications using NB100-104 Applications Species
Chakravarti D, Hu B, Mao X et al. Telomere dysfunction activates YAP1 to drive tissue inflammation Nat Commun Sep 21 2020 [PMID: 32958778] (WB, Mouse) WB Mouse
Morita A, Aoshima K, Gulay KCM et al. High drug efflux pump capacity and low DNA damage response induce doxorubicin resistance in canine hemangiosarcoma cell lines Res. Vet. Sci. 2019 Oct 17 [PMID: 31648115] (WB, Canine, Human) WB Canine, Human
Pugliese GM, Salaris F, Palermo V et al. Inducible SMARCAL1 knockdown in iPSC reveals a link between replication stress and altered expression of master differentiation genes Dis Model Mech Sep 12 2019 [PMID: 31515241] (WB, Human) WB Human
Karki A, Putra J, Kim SS et al. MDM4 expression in fibrolamellar hepatocellular carcinoma Oncol. Rep. Jul 18 2019 [PMID: 31322272] (WB, IHC, Human) WB, IHC Human
Yin Y, Xu L, Chang Y et al. N-Myc promotes therapeutic resistance development of neuroendocrine prostate cancer by differentially regulating miR-421/ATM pathway. Mol. Cancer Jan 18 2019 [PMID: 30657058] (WB, IHC, Human) WB, IHC Human
Dworak N, Makosa D, Chatterjee M et al. A nuclear lamina-chromatin-Ran GTPase axis modulates nuclear import and DNA damage signaling Aging Cell Dec 19 2018 [PMID: 30565836] (ICC/IF, Human) ICC/IF Human
Xu L, Ma E et al. ATM deficiency promotes progression of CRPC by enhancing Warburg effect. Endocr Relat Cancer Jan 1 2019 [PMID: 30400006] (WB, Human) WB Human
Hashimoto T, Murata Y, Urushihara Y et al. Severe hypoxia increases expression of ATM and DNA-PKcs and it increases their activities through Src and AMPK signaling pathways. Biochem. Biophys. Res. Commun. Sep 15 2018 [PMID: 30224064] (WB, Human) WB Human
Gu X, Qi Y, Feng Z et al. Lead (Pb) induced ATM-dependent mitophagy via PINK1/Parkin pathway. Toxicol. Lett. Apr 13 2018 [PMID: 29660402] (WB) WB
Murata Y, Hashimoto T, Urushihara Y et al. Knockdown of AMPKa decreases ATM expression and increases radiosensitivity under hypoxia and nutrient starvation in an SV40-transformed human fibroblast cell line, LM217. Biochem. Biophys. Res. Commun. 2017 Dec 25 [PMID: 29284117] (WB, Human) WB Human
Show All 90 Publications.

Reviews for ATM Antibody (NB100-104) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.

Reviews using NB100-104:
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Images Ratings Applications Species Date Details
reviewed by:
Barbara Alcaraz Silva
IF Other 10/08/2014


Sample Testedcell


Fixation Details4% PFA, RT
Western Blot ATM NB100-104
reviewed by:
Christie Brown
WB Human 08/15/2013


ApplicationWestern Blot
Sample TestedLymphoblastoid (LCL) nuclear lysate
CommentsThis ATM antibody is very clean and specific. It does not show an ATM band in lymphoblastoid cell lines from A-T patients. It usually requires a 5-10 min exposure. I get better results from nuclear lysates than from whole cell lysates.


Blocking Details5% milk in PBSt

Primary Anitbody

Dilution Ratio1/1000, overnight, 5% milk in PBSt

Secondary Antibody

Secondary Description5% milk in PBSt
Secondary Manufacturer Cat#45-000-682
Secondary Concentration1/3000


Detection NotesAmersham ECL, 10min


CommentsThis ATM antibody is very clean and specific. It does not show an ATM band in lymphoblastoid cell lines from A-T patients. It usually requires a 5-10 min exposure. I get better results from nuclear lysates than from whole cell lysates.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATM Antibody (NB100-104). (Showing 1 - 4 of 4 FAQs).

  1. What is the theoretical molecular weight for your ATM antibodies?
    • The theoretical molecular weight for our ATM antibodies is 351 kDa.
  2. What is the expiration date of this product?
    • We guarantee the stability of this product for 1 year from the date of receipt.
  3. What is the isotype of this antibody?
    • The isotype for this ATM Antibody is IgG.
  4. Which applications can I use this antibody in?
    • This antibody has been cited in multiple publications for use in ELISA, flow cytometry, immunoblotting, ICC/IF, immunohistochemistry, immunoprecipitation, and western blot. This antibody has also been tested in IHC-Paraffin; therefore all of these applications fall under our 100% guarantee. If you would like to test this antibody in an application or species not listed, please check out our Innovator's Reward Program.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 NB100-104AF405
Alexa Fluor 488 NB100-104AF488
Alexa Fluor 647 NB100-104AF647
Alexa Fluor 700 NB100-104AF700
Biotin NB100-104B
DyLight 405 NB100-104V
DyLight 488 NB100-104G
DyLight 550 NB100-104R
DyLight 594 NB100-104DL594
DyLight 650 NB100-104C
FITC NB100-104F
HRP NB100-104H
PE NB100-104PE

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Blogs on ATM.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

ATM and DSB Repair in Cancer
Ataxia Telangiectasia Mutated (ATM) is a serine/threonine protein kinase that is the master regulator of the DNA double-strand break (DSB) repair pathway. ATM is a key part of the cell cycle machinery that activates checkpoint signaling in response to...  Read full blog post.

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Recent Reviews


Barbara Alcaraz Silva
Application: IF
Species: Other

Christie Brown
Application: WB
Species: Human


Gene Symbol ATM